Vol.2 No.10 -1 : Effect of anti-sca-1+ on diabetic rat histological, immunohistochemical and biochemical evaluation.

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By : Bahgat El-Fiky , Saber A. Sakr , Sobhy Hassab El Nabi , Mona El Gawish , Asmaa Uonis and Ahmed EL Kattan.

Abstract

Diabetes mellitus is a disease of metabolic dysregulation most notable glucose metabolism accompanied by long term complications. The present study was carried out to investigate the potential effect of Anti-Sca-1+ stem cells on type 1 diabetes induced in albino rats. The study was carried on 7 male and 24 female Albino rats (150 ± 5g). The female albino rats were divided into three main groups: normal control, diabetic group and diabetic rats treated by Anti-Sca-1+. Induction of diabetic rats was carried out by single dose injection of fasting rats for 48 h with a diluted solution of 40 mg/kg of alloxan monohydrate (2%) in freshly prepared saline solution. Diabetic rats treated group was slaughtered into two phases; the first phase after 15 days of treatment, and the second phase after 30 days of treatment. Tissue samples were separated and kept in absolute 10% formalin solution for histological and immunohistochemical studies. SRY gene expression and biochemical study of glucose tolerance, c-peptide, insulin, glycated hemoglobin and glucose transporter 2 was evaluated. The results showed that, treatment of diabetic rats, with derivative bone marrow (Anti-Sca-1+) could greatly ameliorate glucose, insulin, c-peptide, HbA1c and glut2, Hafter 15 and 30 days of treatment, as compared to normal control group and these were confirmed by histological and immunological investigations, which indicate significant progress in the pancreas tissue, by marked significant increase of pancreatic islets size and improvement in the acinar cells after 30 days of treatment by Anti-Sca-1+.

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Vol.2 No.10 -2 : Oral supplementation of aqueous Ginkgo biloba extract inhibits oxidative stress and hippocampus injury associated with methotrexate injection.

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By : Ehab Tousson , Ahmed Masoud; Afaf M. Elatrsh and Tamer Mostafa

Abstract

Methotrexate is effective for the treatment of a number of cancers including breast, lung, leukemia an osteosarcoma. This study was conducted to examine the possible modifying effects of Ginkgo biloba against hippocampal and brain toxicity induced by methotrexate in male albino rats. This could be fulfilled through the histological, immunohistochemically and biochemical analysis of hippocampal brain tissues. The experiments were performed on 36 male albino rats divided into 6 groups; the first and second groups were the control and Gingko groups respectively while the 3rd group methotrexate rat group; the 4th and the 5th groups were co- and post treated Methotrexate rat groups with Gingko respectively and the 6th group was methotrexate self-treated rat group. In brain homogenates Malondialdehyde (MDA) levels in methotrexate group showed a significant increase when compared with control group, in contrast methotrexate-treated group also exhibited a significant decrease in brain antioxidants machinery represented by catalase, reduced glutathione (GSH) and total proteins. The histological changes consisted of destruction of oligodendrocytes, sometimes complete over large areas, and sometimes relatively slight. All the rats which survived long enough after treatment showed severe astrocytosis. Rats’ brains immunocytochemical results showed that GFAP-positive cells astrocytes increase after Exposure to (MTX) compared with the other groups.

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Vol.2 No.10 -3 : Effect of calcium ionophore A23187 on the sperm in intracytoplasmic sperm injection.

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By : Abd El-Wahab El-Ghareeb , Ibrahim Yahia Abd el-Kadr , Amr Tharwat Al Ahwany , Aziza Mansour Ali and Ola Ismail Hassan

Abstract

The present study evaluated the effect of calcium ionophore A23187 on sperm in intracytoplasmic sperm injection (ICSI) cycles using sperm from different sources. The 80 cycles evaluated were divided into four subgroups according to sperm quality and origin (normospermia- oligoathenoteratospermia–epidermal–testicular), subgroups were further split into experimental groups, depending on whether the spem treated or not treated with calcium ionophore. For each experimental subgroup, ICSI outcomes were compared between groups. In conclusion we found that using calcium ionophore increase the fertilization rate in case of sperm treatment with calcium ionophore before ICSI for oligoathenoteratospermia (OAT) & testicular subgroups, with enhancing embryos quality in testicular subgroup only .

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Vol.2 No.10 -5 : Modulation of boldenone induced hepatic and renal toxicity by Moringa oleiferaas in albino rats.

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Abstract

Boldenone is an anabolic androgenic steroid and synthetic derivative of testosterone that was originally developed for veterinary use. Its use is very spread on veterinary medicine because its ability to increase protein synthesis. The aim of this study is to show the toxic effect in liver and kidney caused after the intramuscular injection of boldenone and focus on the role of Moringa oleifera as co-trateated substance in improving hepatic and renal toxicity of boldenone. 40 adult rats were equally divided into four main groups. Group A injected intramuscularly with olive oil, group B treated only with Moringa oleifera 200 mg/Kg body weight, group C injected with boldenone undecylenate only once every three weeks, and co-treated group D which received both intramuscular boldenone undecylenate once every three weeks beside intragastrically dose of of Moringa leaf extract twic-=0lie/week. The results showed that all the animals in the control groups (A and B) appeared healthy till the end of the experiment. The groups treated with boldenone showed a significant elevation in the levels of serum aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), total protein, urea, and creatinine compared to the control group. While the oxidative stress in the groups treated with boldenone showed a significant increase in the level of Malondialdehyde (MDA), nitric oxide (NO), total protein, and total thiol and marked reduction in the level of Glutathione (GSH), Catalase activity (CAT), superoxide dismutase activity (SOD). On the other hand the groups treated with Moringa olifera showed a marked reduction in the level of ALT, AST, urea, creatinine, MDA, and NO. While the level of GSH, CAT, and SOD showed a significant increase comparing with the control group. These results explain the side effect of boldenone undecylenate on the liver and kidney which may cause hepatic and renal diseases and also the role of Moringa olifera in improving these results.

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Vol.2 No.10 -6 : Protective effect of garlic on methyl ethyl ketone-induced biochemical changes in male rabbits.

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By : Zuhair Y. A1-Sahhaf.

Abstract

The present study evaluated the effect of inhalation of methyl ethyl ketone (MEK) on biochemical parameters of rabbits and the possible protective role of garlic aqueous extract. Animals were divided into 4 groups. Group 1 served as controls, group2 given garlic aqueous extract (0.2mg/ml, for a week), group 3 were inhaled (MEK) (500 ppm for 3 minutes daily for 4 weeks), group 4 inhaled MEK for 4 weeks and given garlic for another week. The results showed that exposing animals to MEK induced significant decrease in RBCs count, hemoglobin, hematocrit percentage and blood platelets. On the other hand, the WBCs count was increased. Triglycerides, cholesterol and transaminases (ALT and AST) were increased in the sera of treated rabbits. Rabbits inhaled MEK for two weeks and given garlic aqueous extract for another week showed an improvement of the hematological and biochemical parameters. It is concluded from the present results that garlic aqueous extract modulated toxicity of MEK and this may be attributed to the antioxidant activity of garlic constituents

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