Vol.6 No.4 – 9: Differential expression of salt tolerance related genes in tomato in response to a low dose of γ irradiation

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By: Reem M. Abd El-Maksoud*1, Mohamed Abdelsattar2, Nouf F. Alsayied3, Hanan M. Mansour*4

  1. Department of Nucleic acid and Protein Structure, Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center (ARC), Giza, 12619, Egypt.
  2. Department of Plant Molecular Biology, Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center (ARC), Giza, 12619, Egypt.
  3. Department of Biology, Faculty of Applied Science, Umm Al-Qura University, Makka, Saudi Arabia
  4. Department of Natural Product Research Department, National Center for Radiation Research and Technology, Egyptian Atomic Energy Authority (EAEA), Cairo, Egypt.

Abstract

Using a low dose of gamma rays (30 Gy), the response of twelve salt tolerance-related genes (SlTAS14, SlNCED1, SlDREB2, SlAREB, SlGR, SlAPX1, SlDELLA, SlJAZ1, SlCU/ZnSOD (SlCSD2), SlFSD, SlTIR1 and SlNHX1) was examined at two concentrations of salt stress (50 and 200 mMNaCl). Real-time reverse transcription-PCR analyses of the examined genes showed different expression profiles in shoot and root tissues. In the case of irrigation by 50 mM NaCl, seven genes (SlAPX1, SlGR, SlTAS14, SlNCED1, SlDELLA, SlJAZ1, and SlCSD2) showed a significant increase in their expression in shoot tissues of the irradiated plants. On the other hand, two genes (SlNCED1 and SlDREB2) showed a significant increase in the root tissues at the same concentration. The potential effect of a low dose of gamma rays on enhancing the salinity response of tomato plants can be observed at 200 mM NaCl, where all genes showed a significant increase in shoot tissues of irradiated plants. Interestingly, nine genes (SlNCED1, SlDREB2, SlAREB, SlAPX1, SlDELLA, SlJAZ1, SlCSD2, SlFSD, and SlTIR1) showed a significant increase in the roots of the irradiated plants compared to non-irradiated plants.

Differential-expression-of-salt-tolerance-related-genes-in-tomato-in-response-to-low-dose-of-γ-irradiation-converted-2

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Vol.6 No.4 – 8:The Correlation between Single Nucleotide Polymorphism of MBOAT7 and PNPLA3 Genes to The Degree of Hepatic Fibrosis in HCV Patients: An Experience from Egypt

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DOI: 10.21608/jbaar.2020.135612

By: Samar Samir Youssef1*, Eman Abd El Razek Abbas1, Yasser Hamada Aly2, Sameh Seif3

  1. of Microbial Biotechnology, National Research Centre, Dokki, Egypt.
  2. Department of Endemic Medicine, Cairo University.
  3. National Hepatology and Tropical Medicine Research Institute, Cairo, Egypt

Abstract

Objectives: To detect the correlation between the single nucleotide polymorphisms (SNPs) in MBOAT7 and PNPLA3 genes and hepatic fibrosis in hepatitis C virus (HCV) Egyptian patients, and to highlight the additive effect, if any, of MBOAT7 on the correlation of PNPLA3 polymorphism with liver fibrosis in HCV patients from Egypt. Methods: In this cross-sectional study, we recruited treatment-naïve patients with chronic HCV. The rs738409 (PNPLA3) and rs641738 (MBOAT7) polymorphisms were assessed by Real-Time PCR. We utilized the METAVIR‐Score to classify the degree of hepatic fibrosis and necroinflammatory activity.

Results: A total of 93 patients (mean age 42.72 ± 10.46; males = 49.5%) were included. Our analysis showed that 10.8% of the patients had GG genotype of the PNPLA3 gene and 46.2% had TT genotype of the MBOAT7 gene.  Compared to combined CC and GC genotypes, carriers of GG genotype in the PNPLA3 gene were more likely to be males (p =0.041), have higher fibrosis grade (p =0.043), have higher serum creatinine (p=0.036), higher TSH (p =0.017) and higher viral load (p =0.045). Notably, we found a significant association between TT genotype in MBOAT7 and advanced fibrosis only (but not with necroinflammation (p >0.05). Our multivariate analysis showed that the GG genotype in the PNPLA3 gene and TT genotype in the MBOAT7 gene were independent predictors of advanced fibrosis. Conclusion: PNPLA3 GG genotype and MBOAT7 TT genotype are independent predictors for hepatic fibrosis, and thus might be linked to faster disease progression.

The Correlation between Single Nucleotide Polymorphism of MBOAT7 and PNPLA3 Genes to The Degree of Hepatic Fibrosis in HCV Patients-converted

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Vol.6 No.5 – 4: Estimation of the potency of the genetic action and the heritability of a set of traits of bread wheat (Triticum aestivum . L)

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By: Omar Abd Ahmed and Jasim Mohamed Aziz Aljobory

Department of Field Crops, Faculty of Agriculture, Tikrit University, Iraq

Abstract

10 Genotypes of bread wheat (Triticum aestivum L.) were planted arranged in sequence (1) Abu Ghraib (2) Cowes (3) Osis (4) Sit Mall (5) Florca (6) Clack (7) Milan (8) Hedab (9) Abaa 99 and (10) Sham. In addition to their interchanged mid-crosses, their number (45), Obtained from crosses between parents without reverse crossbreeds, These structures were planted to study (parents + first-generation hybrids) in Diyala Governorate – Baldruz District, for the agricultural season (2017-2018) and using the design of (RCBD) and with three replications and traits (number of days to expel spikes and Plant height is cm, leaf area (cm2), number of grains per spike, number of spikes/plant -1, the weight of 1000 grains (g), individual plant yield (g), biological plant yield (g), it was found that the parents and their hybrids differed significantly for all traits. The study studied and the hybrid (7 × 9) showed a desirable hybrid strength for most of the studied traits from the average parents and the best parents, while the hybrid (2 × 6) showed a desired hybrid strength in four traits, including the grain yield as a deviation from the best parents. Sovereign variation, which was reflected in the degree of sovereignty, as it reached more than one correct one for all traits, which confirms the superior sovereignty that controls its inheritance, and that the proportion of the dominant and recessive alleles is less than 0.25%, meaning that it does not show the regular distribution in the sites that show dominance and that the degree of heritability in the narrow sense is less than 20%, which confirms its lack of importance Additional variability of the genetic sites that control the inheritance of these traits.

تقدير قوة الفعل الجيني والتوريث لمجموعة من الصفات لحنطة الخبز (1)

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Vol.6 No.5 – 3: Determining radon concentrations naturally emitted from water samples, Sediments, Organisms, and plants of chosen areas from the south of Iraq

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By: Master A. Abdul Ali*, Ali Abdul Hussein

Department of Environment, Basra Oil Company, Ministry of Oil, Iraq

Department of Science, Basic education college, University of Misan

Abstract

The study gathers 56 samples of waters, Sediments, bones of some Living being as well as some plants available in local markets (vegetables and fruits) taken from different areas of the south Iraq governorates to determine radon concentration  222Rn emitted from the chosen samples and the generating radioactive radium 226Ra resulted from the dissolution of the period  Jan – March 2019. It manipulates the active measure method of the electronic speeder through the use of the Rational Application Developer RAD7 and the passive method, the long-term method for radiation of alpha particles, utilizing Solid State Nuclear Track Detectors (SSNTDs) technique and Two types of detectors were used; CR-39 and LR-115-typeII. The results show that the greatest concentrations of radon in waters 16234±1014Bq/m3 in Shatt- El-Arab waters in Basra while the least concentration 2153±134Bq/m3in Al-Turbia station in Thiqar, whereas the highest radon concentration 20570±1285Bq/m3 is of a sample taken from the Sediments if Al- Msab_el_aamin the north of Basra. As for the concentration in living beings, the highest is of a sheep bone 4869±304 Bq/m3 from Basra (Northern Rumaila); the least is of a chicken bone 132±8 Bq/m3   taken from local markets in Misan. The planet samples show that the highest radon concentration 128± 8Bq/m3 is fenugreek from Basra markets and the least is of an apple exported in Misan markets. The annual estimated dose affecting water is1.60mSvy-1 Which is greater than the worldly permitted dose, which is 1mSvy-1. Generally, it is found that radon concentration of the chosen water samples from the South of Iraq is greater than the permitted ratio, 11Bq/L and its concentration in Sediments also records a noted increase which may cause danger for population health living in these areas.

تحديد تراكيز غاز الرادون المنبعث طبيعيا من عينات مياه ورواسب وكائنات حية ونباتات في مناطق منتخبة من جنوب العراق (1)

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Vol.6 No.4 – 7: Biochemical screening of low energy Infrared laser beam irradiation effect on the liver and kidney function in male albino mice

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DOI: 10.21608/jbaar.2020.134935

By: Moustafa Hussein Moustafa

Biophysics Department, Medical research institute, Alexandria University Egypt

Abstract

Herein we conduct biochemical screening of the low energy Infrared laser beam effect (frequency: 3 and 7 kHz) on the liver and kidney of male albino mice as complementary investigation effect therapeutic ultrasound. The histopathological examination of organ tissues revealed a homogenous normal structure. Hematological investigation of complete blood count (CBC) was carried out and platelet count showed a significant (p<0.05) decline in irradiated sample tissues relative to control. Tissue-specific blood enzymes (aspartate aminotransferase (SGOT), serum creatinine, and blood urea) were still around normal levels. The rate of oxidative stress in blood as glutathione reductase (GR), and total antioxidant capacity (TCA) were estimated. Malondialdehyde (MDA) as a lipid peroxidation indicator was determined as well.  Conclusively, we may conclude that low energy I.R. laser has no aberrant harmful effect on the liver and kidney of male albino mice under the current experimental conditions.

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” title=”Biochemical screening of low energy Infrared laser beam irradiation effect on the liver and kidney function in male albino mice-converted”]

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Vol.6 No.4 – 6: Impact of JAK2V617F Mutational on Haematologic Features in Sudanese Patients with Essential Thrombocythemia and Thrombotic Risk Assessment

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By: Sahar G. Elbager1,2*, Magdi A. Bayoumi3, Eusni Rahayu Mohd Tohit2, Amar A. Dowd1,4, Mei I Lai2

1Department of Hematology, Faculty of Medical Laboratory Sciences, University of Medical Sciences and Technology (UMST), Khartoum, Sudan.

2Department of Pathology, Faculty of Medicine and Health Sciences, University Putra Malaysia (UPM), Selangor, Malaysia

3Faculty of Postgraduate Studies, Scientific Research, and Publishing, Ibn Sina University (ISU), Khartoum, Sudan

4Department of Pathology, Faculty of Medical Laboratory Sciences, University of Medical Sciences and Technology (UMST), Khartoum, Sudan

Abstract

Objective: We correlated selected hematological parameters in Sudanese essential thrombocythemia (ET) patients based on their homozygous/heterozygous JAK2V617F genotype, as well as the application of thrombotic risk assessment using different thrombotic risk scoring models. Methods: In this single-center study, we evaluated 60 patients with ET at the time of the diagnosis without any prior treatment. Amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) technique was used to determine JAK2V617F mutation status. Complete blood count was evaluated using the Sysmex analyzer.  Furthermore, the thrombotic risk assessment of ET patients using different thrombotic risk scoring models was applied.

Results: The JAK2V617F mutation was detected in 29/60 patients (48.3%), of whom 23 (38.3% of total) were heterozygous and 6 (10.0%) were homozygous.  Compeered to JAK2 wild-type or JAK2 heterozygous patients, JAK2 homozygous patients for JAK2V617F mutation were associated with older age(p<0.05), significantly higher mean leukocytes count (P =0.001), significantly lower Hb concentration (p<0.05), and splenomegaly (p<0.05), while the mean of the platelet counts was slightly higher, although not reached a significant level. We also found two patients who developed thrombotic events throughout follow-up and were initially classified as a low-risk category in the traditional classification. One of them with age < 60 years, hypertension, and JAK2 homozygosity but without thrombosis history, was allocated in a high-risk category by IPSET-t and r- IPSET-t scores. The second patient was stratified in a low-risk category by all scoring models with age < 60 years, hypertension, leukocytosis, unmutated JAK2, and without a history of thrombosis.

Conclusions: The JAK2 V617F homozygosity correlated with older age, higher leukocyte count, lower Hb concentration, and a higher risk of thrombosis in Sudanese ET patients. Evaluation of hypertension and identification of JAK2 V617F homozygosity at diagnosis of ET might give the clinician more meaningful prognostic information and so improve the therapeutic management.

Impact of JAK2V617F Mutational on Haematologic Features in Sudanese Patients with Essential Thrombocythemia and Thrombotic Risk Assessment-converted

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Vol.6 No.4 – 5: An abundance of soil invertebrates in young and mature palm oil plot in relations to soil physical properties: A preliminary study

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By: Nurul Hazirah Hamzah1*, Abrar Maqtan1, Hishamuddin Omar1, and Hafiz Ibrahim1

1Department of Biology, Faculty of Science, Universiti Putra Malaysia, UPM, Serdang, Selangor 43400, Malaysia

Abstract

The soil biota is considered the biological engine of the earth and is associated with most of the critical functions of the soil in terms of ecosystem services. The knowledge on the impact of land management and agricultural activities on the abundance and diversity of soil invertebrates is still understudied especially in Malaysia. This study was conducted to investigate the relation of the abundance and diversity of soil invertebrates with the soil physical parameters from two different palm oil plots. Sampling methods of soil invertebrates include pitfall trap and Berlese-Tullgren funnel. Invertebrate’s abundance and diversity were measured and invertebrates were identified according to their morphological characteristics under the dissecting microscope concerning taxonomic keys. A total of 621 individuals were documented from both sites with 408 collected in mature palm oil farms whereas 213 individuals were documented in young palm oil farms. Hymenoptera (67.31 %) is the most dominant invertebrate followed by Orthoptera (12.40 %) and Arachnida (10.14 %). Morphospecies diversity, evenness, and richness are highest in mature palm oil plot with Shannon; 1.54, Evenness; 0.32, and Menhinick; 0.67 respectively. Well-developed canopy, plenty of shelters, and reduced agricultural activities in mature palm oil mainly contributed to ideal soil physical parameters (bulk density; 1.23 (g/cm3) ± 0.02, porosity; 55.03 % ± 2.48, temperature; 35.65°C ± 0.30 and moisture; 34.05 % ± 0.60) produces a comparatively more balanced habitat for a diverse community of soil invertebrates. Because of the roles they play in soil biological processes, soil invertebrates may be used as bioindicators of soil health. This study can be used as a baseline for more extensive research that may be applied in future planning on land management in achieving the nation’s agricultural sustainability.

An abundance of soil invertebrates in young and mature palm oil plot in relations to soil physical properties A preliminary study-converted

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Vol.6 No.5 – 2:Effect of seawater and salicylic acid on the activity of antioxidant enzymes in the embryonic callus tissue of the date palm plant (Phoenix dactilylfera L.) cultivar Barhi cultivated ex vivo

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By: Abdalamir Rahim Ebed1 and Khayon Ali Mohsin2

1. Faculty of Agriculture, University of Basrah, Iraq

2. Palm Research center, University of Basrah, Iraq

Abstract

This study was conducted in the laboratories of the Palm Research Center of the University of Basrah. The study studied the effect of different concentrations of seawater (0, 10, 20, 30, and 40%) and different concentrations of Salicylic acid (0, 50, and 100 mg / L) (MS) with a concentration of 4.6 g / l to add some amino acids and some vitamins and use of NAA at a concentration of 10 mg / L and  2,4.D at a concentration of 20 mg / L. The results of the study showed that increased seawater in the medium increased In the concentration of amino acid proline and phenols and significantly reduced In the total carbohydrate either increased the concentration of salicylic acid has led to a significant increase in total carbohydrates and phenols and significantly reduced in the amino acid proline. As for the effect of seawater on the actual antioxidant enzymes, the results of the present study showed that its increased concentration in the medium resulted in a significant increase in the effectiveness of SOD, CAT, and APX enzymes and significantly reduced the effect of enzymes (PO and GR) salicylic acid increased in the medium to a significant increase in all enzymes studied.  

تاثير ماء البحر وحامض السالسليك في فعالية الانزيمات المضادة للاكسدة-converted

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Vol.6 No.5 – 1: Economic analysis to factors affecting the production of fish farms – mud ponds in the province of Diyala for the season of 2018

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By: Abbas Abid Ahmed Al Tamimi and Ali Yasin Khudair

ABSTRACT 

     The study examined the production function of the fish farms in the Diyala Governorate for the season 2018 through a questionnaire prepared for this purpose, questionnaire (31) fish products were selected from (61) products while (27) farms were not working out of the total, Multivariate analysis was carried out by taking the natural logarithm of production as a dependent factor and the natural logarithm of the productive resources as independent factors. The analysis revealed the importance of conservation and irrigation costs, fish feed and prevention costs and the cost of purchasing and transferring the fingerlings. These resources were positive elasticities, while the analysis proved the significance of the labour resource of the elasticity was negative. The analysis of the regression of both capital and land and labour using the model (Cup – Dogalas) to determine the overall elasticity of production and the analysis proved the significance of these resources were positive elasticities, which means that these resources gave the optimal combination in the model (Cup – Douglas). The descriptive analysis also revealed the lack of government support for these farms and the lack of hatching stations. It was recommended that government support for fish producers such as providing hatching stations and activating agricultural extension to raise awareness of farmers at the prices of production inputs and applying the results of scientific research to farm management. Production to diversify farmers’ income and make use of available resources.

تحليل إقتصادي وقياسي للعوامل المؤثرة على انتاج مزارع الاسماك-converted

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Vol.6 No.4 – 4: Bio-Remediation of Pb-contaminated Soil Cultivated With Faba Bean Via Application of Rhizobacteria Bacillus amyloliquefaciens subsp. plantarum UCMB 5033

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By: Rania Mohammed Sabri Sultan1, Raad Homod Mohammed Al-Hazmi2

1Department of Biological Sciences, Faculty of Science, King Abdulaziz University Jeddah, 21589, Saudi Arabia2Ministry of Education, Western Region, Jeddah, Saudi Arabia

Abstract

Bio-remediation of Faba bean (Vicia faba cv. luz deotono) plants growing under lead (Pb) contaminated soil using the bacteria strain Bacillus amyloliquefaciens subsp. plantarum UCMB 5033 was tested under three Pb concentrations (2000mg/L 0.5%, 2500mg/L 1.0%, 3000mg/L 5%). Growth parameters (plant height, plant fresh weight, and plant dry weight of the Pb-treated bacteria inoculated Faba bean plants showed significant increases compared to the Pb-treated non-inoculated Faba bean plants under Pb concentration of 0.5% and 1.0% giving 5.76%, 50.43% increases in height, 42.68%, 253.36% increase in fresh wt., and 237.69%, 325.97% increase in dry wt. Regarding the plant nutrient, NPK contents the non-inoculated Pb–treated plants gave significantly the highest N content under all Pb concentration levels and the highest PK contents under Pb concentrations 0.5 and 1.0% compared to the bio-remediated plants. But the bio-remediated plants dominated the non-inoculated Faba bean plants in absorbing the highest PK contents under a Pb concentration of 5.0%. Regarding Pb absorption, the bio-remediated plants dominated the non-inoculated Faba bean plants and absorbed the highest Pb especially under 1.0% Pb concentration. Bacillus amyloliquefaciens subsp. plantarum UCMB 5033 may be considered a bio-remediator for Faba bean plants grown under Pb contaminated soils.

Bio-Remediation-of-Pb-contaminated-Soil-Cultivated-With-Faba-Bean-Via-Application-of-Rhizobacteria-Bacillus-amyloliquefaciens-subsp.-plantarum-UCMB-5033-converted

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