Vol.9 No.2-3:COVID-19 Laboratory diagnosis methods

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By: Ahmed Abdelhalim Yameny

Society of Pathological Biochemistry and Hematology, Egypt

Abstract

Coronavirus-19 is caused by “Severe acute respiratory syndrome coronavirus-2” (SARS-CoV-2), COVID-19 pandemic, has led to millions of confirmed cases and deaths worldwide. Efficient diagnostic tools are in high demand, as rapid and specific testing plays an important role in patient management and decelerating disease spread. the current technologies used to detect COVID-19 in clinical laboratories will be classified into main three categories, I– The polymerase chain reaction (PCR) method, II– Immunological or serological methods (Antigen and Antibody Detection), III– Biochemical and hematological methods which include 1- Inflammatory biomarkers (Elevated and decreased group), 2.Multi-organ damage/failure (Cardiac, Hepatic, and Renal).

COVID-19-Lab-Diagnosis-methods

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Vol.9 No.2-2:Urtica pilulifera leaves exacerbate the cisplatin effect in Ehrlich ascites carcinoma-bearing mice

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By: Karim Samy El-Said*, Asmaa Reda Abdo Mahmoud Mohamed, Amro El-Sherbeni Mohamed

Biochemistry Division, Chemistry Department, Faculty of Science, Tanta University, Egypt

Abstract:

Conventional chemotherapy is an effective approach to cancer treatment. However, several side effects limited its uses. Natural products have been reported for their anticancer potential. This study evaluated the anticancer efficacy of Urtica pilulifera leaves extract (UPLE) alone or in combination with the Cis chemotherapeutic drug Cis in Ehrlich ascites carcinoma (EAC)-bearing mice. Phytochemical constituents were determined in UPLE by quantitative methods. Seventy mice were divided into seven groups (n = 10) as follows: Gp1 was used as a negative control, from Gp2 to Gp7 were inoculated with 1 × 106 EAC-cells/mouse, then Gp2 left as a positive control, Gp3 was injected with Cis (2 mg/kg), Gp4 was injected with UPLE (100 mg/kg), Gp5 was co-treated with Cis as Gp3 and UPLE as Gp4. Gp6 was injected with a low dose of Cis (0.5 mg/kg), and Gp7 was co-treated with a low dose of Cis as Gp6 and UPLE as Gp4. The body weight change percentages (b.wt%) were calculated. On day 14, all groups were sacrificed, the ascitic fluids were harvested, and the total tumor volume, count, and live and dead tumor cells were measured. Sera samples were collected for biochemical parameters assessment. Liver tissues were collected for the determination of oxidants/antioxidants biomarkers. The results showed that combinatorial treatment of the high or low doses of Cis with UPLE led to a synergistic effect on the reduction in the % b.wt changes due to the decrease in the ascitic tumor fluid. Cotreatment with Cis and UPLE exacerbates the antitumor efficacy of Cis with a significant decrease in liver dysfunctions induced by Cis and enhances the hepatic antioxidant status.

Urtica-pilulifera-leaves-exacerbates-cisplatin-effect-in-Ehrlich-ascites-carcinoma-bearing-mice

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Vol.9 No.2-1:Assessment of cbiL gene expression and vitamin B12 levels in acne vulgaris: involvement of both in-vivo and in-vitro studies

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By:Aly Atef Shoun1*, Ahmed Abd El-Naser Mohamed2* ,Gina Salah Fouad3, Abd-El Aziz El Rifaie4, Naglaa Abd-El Moniem Radi3

1  Microbiology and Immunology Department, Faculty of Pharmacy, Sinai University, El Arish 45518, Egypt

2 Biochemistry Department, Faculty of Pharmacy, New Mansoura University, Egypt

3  Medical Microbiology and Immunology Department, Faculty of Medicine, Beni-Suef University, Egypt

Dermatology Department, Faculty of Medicine, Beni-Suef University, Egypt

Abstract:

Acne vulgaris (AV) is a chronic inflammatory disease of the pilosebaceous follicles that affects nearly 90% of teenagers; half of them continue to experience symptoms as adults. There have been several reports of monomorphic acneiform eruptions in patients treated with intramuscular cobalamin injections. The eruptions resolved after the cessation of the therapy. Cyanocobalamin, pyridoxine (B6), and riboflavin (B2) have been reported to exacerbate existing acne. Moreover, it was reported that in acne patients, the serum level of vitamin B12 was significantly decreased after treatment, but no explanation was given. We propose a vitamin B12-mediated bacterial mechanism for acne pathogenesis on the evidence mentioned above. One hundred twenty AV patients were recruited.

   Our results proved that vitamin B12 was involved in the pathogenesis of AV; in vivo, it proved that AV patients had significantly higher serum levels of the vitamin than controls and showed a positive correlation to the disease duration and severity. In vitro, it proved that vitamin B12 supplementation to p. acnes cultures significantly raised the porphyrins produced in these cultures when compared to the non-supplemented ones. It also proved that this shift in the metabolism is secondary to inhibition of the cbiL gene of the vitamin B12 synthesis in the bacteria; as porphyrins and vitamin B12 share the same precursors, vitamin B12 does has a role in either elicitation or aggravation of AV.

Assessment-of-cbiL-gene-expression-and-vitamin-B12-levels-in-acne-vulgaris-involvement-of-both-in-vivo-and-in-vitro-studies-1

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