Vol.2 No.10 -3 : Effect of calcium ionophore A23187 on the sperm in intracytoplasmic sperm injection.

By : Abd El-Wahab El-Ghareeb , Ibrahim Yahia Abd el-Kadr , Amr Tharwat Al Ahwany , Aziza Mansour Ali and Ola Ismail Hassan

Abstract

The present study evaluated the effect of calcium ionophore A23187 on sperm in intracytoplasmic sperm injection (ICSI) cycles using sperm from different sources. The 80 cycles evaluated were divided into four subgroups according to sperm quality and origin (normospermia- oligoathenoteratospermia–epidermal–testicular), subgroups were further split into experimental groups, depending on whether the spem treated or not treated with calcium ionophore. For each experimental subgroup, ICSI outcomes were compared between groups. In conclusion we found that using calcium ionophore increase the fertilization rate in case of sperm treatment with calcium ionophore before ICSI for oligoathenoteratospermia (OAT) & testicular subgroups, with enhancing embryos quality in testicular subgroup only .

3_Effect_of_calcium_ionophore_A23187_on_the_sperm_in_intracytoplasmic_sperm_injection

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