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Vol.10 No.1 – 7: A Prospective study: Correlation between Serum Estradiol levels and Fertilization Rates in Assisted Reproductive Technology Outcomes

Namir I. A. Haddad 1, Ekhlas Ali Hussein2, Israa Talib Hassan 3

  1. Professor. Department of Chemistry, College of Science, University of Baghdad, Baghdad, Iraq
  2. FICOG. Professor, Department of Gynecology and Obstetrics, College of Medicine, Al-Iraqia University, IRAQ
  3. FICOG. Professor, Department of Gynecology and Obstetrics, College of Medicine, Al-Iraqia University, IRAQ.

ABSTRACT

Background and Objective: Advancements in reproductive procedures require an understanding of factors influencing in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) outcomes. The study objective is to evaluate the impact of serum estradiol (E2) levels and fertilization rates on the success of IVF/ICSI in infertile women.

Materials and Methods: The study comprised one hundred and twenty infertile women undergoing IVF/ICSI treatment.  Hormone levels were measured using enzyme-linked immunosorbent assays (ELISA). The fertilization rate (FR) was estimated by dividing the fertilized oocytes by the total number of microinjection oocytes. Based on the IVF/ICSI outcomes, participants were categorized into pregnant and non-pregnant groups.

Results: The pregnant group had a significantly higher mean E2 level (1480.84 ± 564.29) than the non-pregnant group (1147.84 ± 440.56) (p < 0.001). There were significant correlations between baseline E2 levels and IVF/ICSI outcomes (r = -0.317, p < 0.001). Furthermore, the pregnant group had a significantly higher mean of FR (72.22 ± 9.56) than the non-pregnant group (44.88 ± 37.17) (p < 0.001). Additionally, significant correlations were found between FR and IVF/ICSI outcomes (r = -0.420, p < 0.001).

Conclusion: Higher E2 levels and higher FR significantly affect the probability of success in IVF/ICSI treatments. More research is needed to substantiate these findings and clarify the underlying processes at work.

A Prospective study Correlation between Serum Estradiol levels and Fertilization Rates in Assisted Reproductive Technology Outcomes

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Vol.2 No.10 -3 : Effect of calcium ionophore A23187 on the sperm in intracytoplasmic sperm injection.

By : Abd El-Wahab El-Ghareeb , Ibrahim Yahia Abd el-Kadr , Amr Tharwat Al Ahwany , Aziza Mansour Ali and Ola Ismail Hassan

Abstract

The present study evaluated the effect of calcium ionophore A23187 on sperm in intracytoplasmic sperm injection (ICSI) cycles using sperm from different sources. The 80 cycles evaluated were divided into four subgroups according to sperm quality and origin (normospermia- oligoathenoteratospermia–epidermal–testicular), subgroups were further split into experimental groups, depending on whether the spem treated or not treated with calcium ionophore. For each experimental subgroup, ICSI outcomes were compared between groups. In conclusion we found that using calcium ionophore increase the fertilization rate in case of sperm treatment with calcium ionophore before ICSI for oligoathenoteratospermia (OAT) & testicular subgroups, with enhancing embryos quality in testicular subgroup only .


3_Effect_of_calcium_ionophore_A23187_on_the_sperm_in_intracytoplasmic_sperm_injection
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