Vol.6 No.4 – 8:The Correlation between Single Nucleotide Polymorphism of MBOAT7 and PNPLA3 Genes to The Degree of Hepatic Fibrosis in HCV Patients: An Experience from Egypt

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DOI: 10.21608/jbaar.2020.135612

By: Samar Samir Youssef1*, Eman Abd El Razek Abbas1, Yasser Hamada Aly2, Sameh Seif3

  1. of Microbial Biotechnology, National Research Centre, Dokki, Egypt.
  2. Department of Endemic Medicine, Cairo University.
  3. National Hepatology and Tropical Medicine Research Institute, Cairo, Egypt

Abstract

Objectives: To detect the correlation between the single nucleotide polymorphisms (SNPs) in MBOAT7 and PNPLA3 genes and hepatic fibrosis in hepatitis C virus (HCV) Egyptian patients, and to highlight the additive effect, if any, of MBOAT7 on the correlation of PNPLA3 polymorphism with liver fibrosis in HCV patients from Egypt. Methods: In this cross-sectional study, we recruited treatment-naïve patients with chronic HCV. The rs738409 (PNPLA3) and rs641738 (MBOAT7) polymorphisms were assessed by Real-Time PCR. We utilized the METAVIR‐Score to classify the degree of hepatic fibrosis and necroinflammatory activity.

Results: A total of 93 patients (mean age 42.72 ± 10.46; males = 49.5%) were included. Our analysis showed that 10.8% of the patients had GG genotype of the PNPLA3 gene and 46.2% had TT genotype of the MBOAT7 gene.  Compared to combined CC and GC genotypes, carriers of GG genotype in the PNPLA3 gene were more likely to be males (p =0.041), have higher fibrosis grade (p =0.043), have higher serum creatinine (p=0.036), higher TSH (p =0.017) and higher viral load (p =0.045). Notably, we found a significant association between TT genotype in MBOAT7 and advanced fibrosis only (but not with necroinflammation (p >0.05). Our multivariate analysis showed that the GG genotype in the PNPLA3 gene and TT genotype in the MBOAT7 gene were independent predictors of advanced fibrosis. Conclusion: PNPLA3 GG genotype and MBOAT7 TT genotype are independent predictors for hepatic fibrosis, and thus might be linked to faster disease progression.

The Correlation between Single Nucleotide Polymorphism of MBOAT7 and PNPLA3 Genes to The Degree of Hepatic Fibrosis in HCV Patients-converted

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Vol.6 No.5 – 4: Estimation of the potency of the genetic action and the heritability of a set of traits of bread wheat (Triticum aestivum . L)

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By: Omar Abd Ahmed and Jasim Mohamed Aziz Aljobory

Department of Field Crops, Faculty of Agriculture, Tikrit University, Iraq

Abstract

10 Genotypes of bread wheat (Triticum aestivum L.) were planted arranged in sequence (1) Abu Ghraib (2) Cowes (3) Osis (4) Sit Mall (5) Florca (6) Clack (7) Milan (8) Hedab (9) Abaa 99 and (10) Sham. In addition to their interchanged mid-crosses, their number (45), Obtained from crosses between parents without reverse crossbreeds, These structures were planted to study (parents + first-generation hybrids) in Diyala Governorate – Baldruz District, for the agricultural season (2017-2018) and using the design of (RCBD) and with three replications and traits (number of days to expel spikes and Plant height is cm, leaf area (cm2), number of grains per spike, number of spikes/plant -1, the weight of 1000 grains (g), individual plant yield (g), biological plant yield (g), it was found that the parents and their hybrids differed significantly for all traits. The study studied and the hybrid (7 × 9) showed a desirable hybrid strength for most of the studied traits from the average parents and the best parents, while the hybrid (2 × 6) showed a desired hybrid strength in four traits, including the grain yield as a deviation from the best parents. Sovereign variation, which was reflected in the degree of sovereignty, as it reached more than one correct one for all traits, which confirms the superior sovereignty that controls its inheritance, and that the proportion of the dominant and recessive alleles is less than 0.25%, meaning that it does not show the regular distribution in the sites that show dominance and that the degree of heritability in the narrow sense is less than 20%, which confirms its lack of importance Additional variability of the genetic sites that control the inheritance of these traits.

تقدير قوة الفعل الجيني والتوريث لمجموعة من الصفات لحنطة الخبز (1)

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Vol.6 No.5 – 3: Determining radon concentrations naturally emitted from water samples, Sediments, Organisms, and plants of chosen areas from the south of Iraq

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By: Master A. Abdul Ali*, Ali Abdul Hussein

Department of Environment, Basra Oil Company, Ministry of Oil, Iraq

Department of Science, Basic education college, University of Misan

Abstract

The study gathers 56 samples of waters, Sediments, bones of some Living being as well as some plants available in local markets (vegetables and fruits) taken from different areas of the south Iraq governorates to determine radon concentration  222Rn emitted from the chosen samples and the generating radioactive radium 226Ra resulted from the dissolution of the period  Jan – March 2019. It manipulates the active measure method of the electronic speeder through the use of the Rational Application Developer RAD7 and the passive method, the long-term method for radiation of alpha particles, utilizing Solid State Nuclear Track Detectors (SSNTDs) technique and Two types of detectors were used; CR-39 and LR-115-typeII. The results show that the greatest concentrations of radon in waters 16234±1014Bq/m3 in Shatt- El-Arab waters in Basra while the least concentration 2153±134Bq/m3in Al-Turbia station in Thiqar, whereas the highest radon concentration 20570±1285Bq/m3 is of a sample taken from the Sediments if Al- Msab_el_aamin the north of Basra. As for the concentration in living beings, the highest is of a sheep bone 4869±304 Bq/m3 from Basra (Northern Rumaila); the least is of a chicken bone 132±8 Bq/m3   taken from local markets in Misan. The planet samples show that the highest radon concentration 128± 8Bq/m3 is fenugreek from Basra markets and the least is of an apple exported in Misan markets. The annual estimated dose affecting water is1.60mSvy-1 Which is greater than the worldly permitted dose, which is 1mSvy-1. Generally, it is found that radon concentration of the chosen water samples from the South of Iraq is greater than the permitted ratio, 11Bq/L and its concentration in Sediments also records a noted increase which may cause danger for population health living in these areas.

تحديد تراكيز غاز الرادون المنبعث طبيعيا من عينات مياه ورواسب وكائنات حية ونباتات في مناطق منتخبة من جنوب العراق (1)

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