By: Bassim K. Kuoti Al-Rekabi *; Mohammed A. Al-Diwan ** ; Alaa A. Sawad ***

* Department of Animal Production, College of Agriculture, University of Sumer, Thi-Qar, Iraq.

** Department of Physiology, Pharmacology and Chemistry, College of Veterinary Medicine, University of Basrah, Basrah, Iraq.

*** Department of Anatomy, Histology and Embryology, College of Veterinary Medicine, University of Basrah, Basrah, Iraq.

Abstract

This study was designed to evaluate the protective role of exogenous CoQ10 and DHEA and their combination on CCl4 induced hepatotoxicity in adult male rats. Thirty adult male rats 225-250 grams, 12-14 weeks old were used in this study and randomly divided into five equal groups, 6 animals each as in the following: Control group (G1): 6 male rats received orally DMSO 0.5ml/animal/day, First treated group (T1): 6 male rats received daily CCl4 1ml/kg (1:1 olive oil, IP), Second treated group (T2): 6 male rats received CCl4 1ml/kg and after 1hour injected daily with CoQ10 200 mg/kg IP, Third treated group (T3): 6 male rats received CCl4 1ml/kg and after 1hour injected daily with DHEA  25 mg/kg IP, Fourth treated group (T4): 6 male rats received CCl4 1ml/kg and after 1hour injected daily with a combination of CoQ10 200 mg/kg + DHEA 25 mg/kg IP. The experiment lasted for 28 successive days. The obtained results illustrated that male rats received CCl4 (1ml/kg) caused a significant increased in hepatic function enzymes AST, ALT and ALP, as well as MDA levels, and caused significant decrease in antioxidant enzyme activity GPx, SOD and CAT levels. In addition, CCl4 also caused various degree of liver damage such as dilation and congestion of central vein with hemorrhage, clear fatty degeneration and infiltration of inflammatory cells compared to the  control group. Whereas, the group that treated with CoQ10 200 mg/kg and DHEA 25 mg/kg showed a significant decreased (P< 0.05) in serum AST, ALT and ALP as well as MDA value, and significant increased in GPx, SOD with declined in CAT levels compared to group treated with CCl4 intoxication. It is also observed from the results that combination of CoQ10 and DHEA it caused a highly significant (P < 0.05) declined in AST, ALT and ALP as well as MDA levels, and significant elevated in GPx, SOD and declined in CAT, and almost return to normal level compared to control. As well as, the histopathological examination on liver revealed that rats treated with CoQ10 and DHEA and their combination had normal central vein and hepatocytes compared to groups treated with CCl4 due to anti-oxidant, anti-inflammatory and anti-apoptotic properties. It has been concluded that CoQ10 and DHEA have an evident protective effect against liver damage induced by CCl4 through improving antioxidant enzyme activity in CCl4 treated group leading to a declined MDA level and reduced lipid peroxidation.

The Protective Role of CoQ10 and DHEA and Their combination on CCl4 Induced Liver Injury In Adult Male Rats (Rattus norvegicus)-converted

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