Vol.5 No.3 – 9 : Comparing the level of some stress biomarkers among smoking and non-smoking healthy adults in Egypt

By: Asmaa Fathi Galal1*, Mai Sabry Saleh2, Nagat Mohamed Amer2, Amal Saad-Hussein2

1Narcotics, Ergogenics and Poisons department, Medical Research Division, National Research Centre, Dokki, Giza, Egypt

2Environmental and Occupational Medicine Department, Environmental Research Division, National Research Centre, Dokki, Giza, Egypt

Abstract

Objective: The causal effect relation between smoking and stress is a subject that invites continuous research.  Hypothetically, investigation of stress biomarkers that are reported to be affected by tobacco intake may give us some explanation of the association between stress and smoking as a habit.  Consequently, the aim of the present study was to assess serum level of some stress biomarkers and compare them among smokers and non-smokers in a sample of Egyptian male health volunteers. Methods: Fifty-nine subjects were enrolled in the study (29 smokers and 30 non-smokers of matched age and gender). We measured serum levels of cortisol and interleukin-6 (IL-6) using ELISA technique, and serum levels of α-amylase, triglycerides (TG), and total cholesterol (TC) using colorimetric methods. Results: Serum cortisol levels were decreased in smokers, and IL-6, TG and TC were significantly higher in smokers than non-smokers, whereas, serum α-amylase did not show significant difference. Serum cortisol showed to be negatively correlated with serum IL-6 in smokers. Conclusions: The present studyassumes that smokers suffer from a state of chronic stress as evidenced by the observed decrease in serum cortisol due to negative feedback effect and increase in levels of serum IL-6, TG and TC. This in turn enhances craving to smoke to face stressors and leads to a vicious circuit that smokers fail to quit smoking.

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Vol.5 No.3 – 8 : Improving Intravenous Medication Administration and Reducing Medication Errors Among Critical Care Nurses at Jordan University Hospital

By: Mahmoud Abdul Hameed Shahin

Al-Ghad International Colleges for Applied Medical Sciences, KSA

Abstract

Background: Medication errors represent a serious problem in the hospital setting and remain a challenge to navigate among hospitalized patients in all departments. Mistakes in medication administration are considered a significant issue that threatens a patient’s safety and may increase their hospital stay, treatment costs, and mortality rate. Medication errors commonly committed by nurses may include medication preparation or administration errors, which are associated with the highest risk areas in nursing practice.Methodology: A pretest-posttest, quasi-experimental, observational design was used. Convenience sampling was employed to include all intravenous medication errors committed by nurses in three ICUs of Jordan University Hospital (pretest: 236 errors and post-test: 68 errors, respectively). A designed incident report was used for data collection. Data collection was carried out simultaneously in the three ICUs during nurses’ preparation and administration of intravenous medications over two months for pretest and posttest data (May and June 2018). A tailored evidenced-based educational program designed using Phillips’s Manual of I. V. Therapeutics: Evidence-based Practice for Infusion Therapy was furnished to all registered nurses utilizing structured classroom lectures and on-the-job training; moreover, educational medals of common medications and illustration posters were used as additional reminders.Results and Conclusion: More than half of nurses were females and held bachelor’s degrees. Half of the observed medication errors were identified in the surgical ICU. Intravenous medication errors observed during the day shift were significantly higher in number than those in the night shift. A significant reduction in the number of medication errors was noted after the implementation of a bundle of interventions (i.e., there was a reduction from 236 errors to 68 errors). Giving (1) an omeprazole push and then (2) administering vancomycin rapidly thereafter, followed by (3) administering omeprazole at the wrong time, were the three most observed medication errors in the ICUs. Most medication errors were not reported officially using incident reports. Based on the category of the intravenous medication error, ‘wrong medication rate’ followed by ‘wrong medication time’, and then ‘mixing the medication with another drug’ were the most prominent errors noticed. The rate of reported medication errors was significantly higher after program implementation. An ongoing surveillance system is required to monitor intravenous medication errors and to know the causes so as to find a solution to further decrease them and their consequences. Also, all nurses should receive an intensive specialized evidence-based educational program about medication handling, utilizing clinical training and frequent reminding.

Improving Intravenous Medication Administration and Reducing Medication Errors Among Critical Care Nurses at Jordan University Hospital-converted (1)

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Vol.5 No.3 – 7 : Downregulation of miR-23a and miR-24 in human hepatocellular carcinoma cells by Sorafenib via transforming growth factor beta 1 in a SMAD dependent manner

By: Eman G. Ayad1, Mohga S. Abdulla*1, Hayat M. Sharada1, Abdel Hady A. Abdel Wahab2, and Abeer M. Ashmawy2.

1Department of chemistry, Faculty of Science, Helwan University, Egypt.

2 Departments of Cancer Biology, National Cancer Institute, Cairo University, Egypt.

Abstract

MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression through post-transcriptional interactions with mRNA. MiRNAs have recently considered as key regulators of various cancers including liver cancer. Sorafenib is one of antitumor drug for treatment of advanced hepatocellular carcinoma. It acts as a multikinase inhibitor suppressing cell proliferation and angiogenesis. This study try to investigate a potential microRNA-based mechanism of action of the drug,by studying the effect of sorafenib on miR-23a and miR-24 levels in HCC cell lines HepG2 /Huh7 and revealing the possible drug mechanism against these oncogenic mi-RNAS,in this study cell viability of cultured HepG2 /Huh7 after treatment with sorafenib were evaluated using Sulphorhodamine-B (SRB) assay, cell cycle and apoptosis estimated by flowcytometry assay. Caspase-3 level was determined using ELISA assay. Moreover, MiR-23a and miR-24 expressions levels analyzed by qPCR. Finally, TGF-β levels and phosorylated smad2, 3 were examined after treatment with sorafenib using ELISA and western blotting. Our data confirmed the Sorafenib inhibition of cell growth in both cell lines which was accompanied by significantly increased in cell apoptosis and cell cycle arrest. Cells treated with sorafenib showed a significant decrease in miR-23a and miR-24 levels in both cell lines. Interestingly,   the change in these oncogenic miRNA was accompanying with a significant decrease of (TGF-β1) and phosorylated smad2, 3 proteins levels. Our study suggested that inhibition of tgf beta pathway in smad dependent manner could be the way characteristic of sorafenib to inhibit the oncogenic miR-23a and miR-24 levels in HCC.  

Downregulation of miR-23a and miR-24 in human hepatocellular carcinoma cells by Sorafenib via transforming growth factor beta 1 in a SMAD dependent manner

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