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Vol.2 No.10 -2 : Oral supplementation of aqueous Ginkgo biloba extract inhibits oxidative stress and hippocampus injury associated with methotrexate injection.

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By : Ehab Tousson , Ahmed Masoud; Afaf M. Elatrsh and Tamer Mostafa

Abstract

Methotrexate is effective for the treatment of a number of cancers including breast, lung, leukemia an osteosarcoma. This study was conducted to examine the possible modifying effects of Ginkgo biloba against hippocampal and brain toxicity induced by methotrexate in male albino rats. This could be fulfilled through the histological, immunohistochemically and biochemical analysis of hippocampal brain tissues. The experiments were performed on 36 male albino rats divided into 6 groups; the first and second groups were the control and Gingko groups respectively while the 3rd group methotrexate rat group; the 4th and the 5th groups were co- and post treated Methotrexate rat groups with Gingko respectively and the 6th group was methotrexate self-treated rat group. In brain homogenates Malondialdehyde (MDA) levels in methotrexate group showed a significant increase when compared with control group, in contrast methotrexate-treated group also exhibited a significant decrease in brain antioxidants machinery represented by catalase, reduced glutathione (GSH) and total proteins. The histological changes consisted of destruction of oligodendrocytes, sometimes complete over large areas, and sometimes relatively slight. All the rats which survived long enough after treatment showed severe astrocytosis. Rats’ brains immunocytochemical results showed that GFAP-positive cells astrocytes increase after Exposure to (MTX) compared with the other groups.

2_Oral_supplementation_of_aqueous_Ginkgo_biloba_extract_inhibits_oxidative_stress_and_hippocampus_injury_associated_with_methotrexate_injection

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Vol.2 No.1 -4 : Ameliorating effect of propolis and moringa extract against equigan induced neurotoxicity and oxidative stress on rat hippocampus.

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By : Ehab Tousson, Ezar Hafez, Ahmed Massoud and Ahmed A. Elfeky

Abstract

In most countries worldwide, Equigan is anabolic steroid that is forbidden for human uses or for meat production. The present study was designed to investigate the ameliorating role of Propolis and moringa leaf extract (MLE) against Equigan induced neurotoxicity and oxidative stress on rat hippocampus. A total of 48 healthy male albino rats weighing 170-200 g and of 10-12 weeks age were divided into 6 groups (8 animals each). 1st Control group includes animals that injected intramuscularly with olive oil for 12 weeks. 2nd and 3rd groups were rats received Propolis and MLE respectively. 4th experimental group include animals that receive intramuscular injections of Equigan for 12 weeks; 5th and 6th groups where rats intramuscular injected with Equigan and treated with Propolis and MLE at the same time respectively. The obtained results indicate that hippocampal nitric oxide (NO), malondialdehyde (MDA), acetylcholine esterase (AChE) and total protein were significantly increased in Equigan group when compared with control group. In contrast hippocampal catalase, SOD and total thiol levels were significantly decreased in Equigan group when compared with control group. On the other hand hippocampal NO, MDA, ACE and total protein in Equigan group were significantly increased when compared with co-treated Equigan with MLE or Propolis groups. In contrast; hippocampal catalase, SOD and total thiol levels were significantly decreased in Equigan group when compared with co-treated Equigan with MLE or propolis groups. We can concluded that; MLE and propolis extract ameliorate the neurotoxicity and oxidative stress on rat hippocampus induces by Equigan.

4. Ameliorating effect of propolis and moringa extract against equigan induced neurotoxicity and oxidative stress on rat hippocampus.

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