Posts

Vol.4 No.4 – 14 : Molecular pathological detection of S. typhimurium by fluorescence in situ hybridization (FISH) in lambs tissue sections

/0 Comments/in , , /by

By : Basim M. jwad  and  Bushra I. AL-Kaisei

Department of pathology and poultry diseases, Collages of Veterinary Medicine, University of Baghdad, Baghdad, Iraq

Abstract

Despite  many different methods used to diagnose Salmonella typhimurium, especially that depended on isolation and  biochemical identification, but use technique, Fluorescence in Situ Hybridization (FISH), as a one method from molecular fashion probe, has been developed and applied for the direct diagnosis of S. typhimurium  in bacterial cell smears of pure cultures, or in formalin-fixed sections, and in paraffin embedded tissue. Therefore, this study was designed to determine  the single bacterial cells in the infected intestine, liver and gallbladder by used (Sal-3 prop), through orally administered for lambs via stomach tube, with a volume of 0.5 ml contain  (1×108 cfu/ml S.typhimurium), after bacterial identification by cultures media mainly Salmonella chromogenic agar, and biochemical diagnosis by [Iraq-CDC/central public health laboratory (CPHL) in the Baghdad province]. So results was concluded that Sal3-prop of FISH technique, was a good instrument for fortuity S. typhimurium in the in histopathological tissue sections.

Molecular pathological detection of S. typhimurium-converted

Download PDF

Vol.3 No.4 – 1: Ecophysiological and Histopathological Impacts of Organic Pollution on Two Freshwater Fish Species, Mansoura City, Egypt.

/0 Comments/in , , /by

By : Sherif H. Abdeen, Abeer E. Abdrabouh, Mohamed I. Mashaly, Ahmed E. Hagras, Ali A. Al-halany

Abstract

Organic pollution is one of environmental hazards, especially in aquatic ecosystems. This study was focusing on levels of total organic carbon (TOC%) in sediment of two freshwater habitats different in quality, as well as tissues including; muscles and gonads of two fish species inhabiting both sites during four seasons of one year of study. Ammar drain as a polluted site showed significantly higher levels of TOC% in both sediment and fish tissues comparing to River Nile as a reference site. Data also illustrated that, fish from polluted site showed remarkable decrease in blood indices; RBCs count, Hb content and Hct%, while WBCs count was mostly elevated, especially during autumn. Accompanying to these results, antioxidant enzymes as superoxide dismutase (SOD) and catalase (CAT) were generally decreased during most seasons in fish samples from Ammar drain. Also, histopathological changes in muscles and gonads were observed, where sever deteriorations in these tissues were more discriminating in fish species from Ammar drain compared to River Nile locality. From the obtained results, polluted water drains affect general health and structure of their inhabiting fish species.

Dr.Abeer Dec 2017

Download PDF