Vol.4 No.4 – 14 : Molecular pathological detection of S. typhimurium by fluorescence in situ hybridization (FISH) in lambs tissue sections

By : Basim M. jwad  and  Bushra I. AL-Kaisei

Department of pathology and poultry diseases, Collages of Veterinary Medicine, University of Baghdad, Baghdad, Iraq

Abstract

Despite  many different methods used to diagnose Salmonella typhimurium, especially that depended on isolation and  biochemical identification, but use technique, Fluorescence in Situ Hybridization (FISH), as a one method from molecular fashion probe, has been developed and applied for the direct diagnosis of S. typhimurium  in bacterial cell smears of pure cultures, or in formalin-fixed sections, and in paraffin embedded tissue. Therefore, this study was designed to determine  the single bacterial cells in the infected intestine, liver and gallbladder by used (Sal-3 prop), through orally administered for lambs via stomach tube, with a volume of 0.5 ml contain  (1×10⁸ cfu/ml S.typhimurium), after bacterial identification by cultures media mainly Salmonella chromogenic agar, and biochemical diagnosis by [Iraq-CDC/central public health laboratory (CPHL) in the Baghdad province]. So results was concluded that Sal3-prop of FISH technique, was a good instrument for fortuity S. typhimurium in the in histopathological tissue sections.

Molecular pathological detection of S. typhimurium-converted

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