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Vol.5 No.4 – 9: Characterization and anti-inflammatory activity effect of exopolysaccharide from Bacillus axarquiensison paw rats carrageenan model

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By: Ghada S. Ibrahim*1,2, Manal G. Mahmoud1, Mohamed E. EL Awady1 Mahgoub M Ahmed3and Samah A Fathy3

1Microbial Biotechnology Department, Genetic Engineering and Biotechnology Research Division, National Research Centre, 33 Bohouth St., Dokki, Giza, 12622, Egypt.

2Department of Biochemistry, College of Science, University of Jeddah, Jeddah, Saudi Arabia

3Molecular Drug Evaluation Department, National Organization for Drug Control and Research (NODCAR), Giza, Egypt

Abstract

The exopolysaccharide (EPS) was produced by a halophilic bacterial strain isolated from marine sediments, Porto-Elsokhna Beach, Egypt. This isolate has been defined morphologically and physiologically and has been recognized by 16S rDNA as Bacillus axarquiensis NRC G6. This strain was deposited in the Gene Bank (http://www.ncbi.nlm.nih. gov) under the accession number of KU945823. The EPS produced was fractionated by precipitation and listed the primary fraction as EPSBa3. Through FT-IR and HPLC, further work attempted to elucidate the structural function of EPSBa3. The findings showed that EPSBa3 was an acidic EPS composed of glucuronic: mannose: galacturonic: glucose and xylose, respectively, in a molar ratio of 1:1:3:3:3.  The proportion of uronic acid and sulfate was 13.9% and 7% respectively. The estimated median molecular weight (Mw) and molecular number (Mn) were 1.499 X106 and 1.29 X 106g / mol. In rats, the anti-inflammatory activity of exopolysaccharide (EPSBa3) was also explored using paw edema induced by carrageenan. EPSBa3 caused important anti-inflammatory effects on the carrageenan model by 50 mg/kg orally. In catalase (CAT) and superoxide dismutase (SOD) enzymes and GSH content, the EPSBa3 enhanced declines. In addition, the content of nitric oxide (NO), lipid peroxidation (LPO) and reactive oxygen species (ROS) arising from carrageenan-induced edema were considerably reduced following therapy with EPSBa3. In addition, the findings showed that interleukin-6 (IL-6) protein expression was decreased after EPSBa3 treatment leading to alleviate paw edema. These findings stated that to avoid inflammatory diseases, EPSBa3 could be utilized as a source of natural products.

Characterization and anti-inflammatory activity effect of exopolysaccharide from Bacillus axarquiensison paw rats carrageenan model-converted

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Vol.4 No.1 – 3 : The possible anti-inflammatory role of the blue green algae ,Aphanizomenon flos-aquae on skin of adult male rats

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By :Hemmat Mansour Abdelhafez and Rasha Mohammed Ibrahim

Abstract

Aphanizomenon flose-aquae (AFA) is a fresh water unicellular blue green microalgae like Spirulina, but most AFA is harvested from the wild in volcanic regions leading to high levels of trace minerals. It has been traditionally used for over 25 years for its health-enhancing properties.
Aphanizomenon flos-aquae is an important source of the blue photosynthetic pigment phycocyanin (PC), which has been described as a strong antioxidant and antiinflammatory agent. Aim of the study: this study aimed to examine the possible anti-inflammatory effect of AFA against the inflammation induced by carrageenan injection on skin of adult male rats using histpathological and histochemical studies.Matrerial and methods: the current experiment was carried out on 48 adult male albino rats (Rattus rattus). Rats were randomly and equally categorized into four groups: 1) Control group (C): rats
were left without treatment; 2) Carr group: rats were injected with carrageenan and left for 21 days ; 3) AFA group: rats were orally administrated Aphanizomenon flos- aquae (AFA) extract (94.5 mg/kg body weight /day) for 21 days and 4) AFA+ Carr Group: rats were injected with carrageenan and treated with 94.5 mg/kg body weight AFA extract daily after six hours of carrageenan injection for 21 days. The experimental rats were sacrificed after 5 and 21 days post– treatment. Results: Examination of skin tissue of rats five and twenty one day’s post-carrageenan injection revealed many histopathological and
histochemical changes such as marked destructed epidermal and dermal layers. The epidermal layer showed undetectable cellular structure, thickened keratin layer. Signs of fibrosis and absence of hair follicles were detected in some areas, in addition to the presence of debris of degenerated cells in the dermal layer. Hair follicles were distorted with numerous fibroblasts in the dermal layer, some of them were hypertrophied, in addition to the presence of large granulomatous area in the dermal layer, discontinuous and faintly stained skeletal muscle fibres were noticed. Most of them showed decreased staining affinity of nuclei of mycocytes (karyolysis) with signs of fatty degeneration. Highly increased collagen fibres and fibrotic areas were detected in the epidermal and dermal layers.
Skin tissues examined five and twenty one days following AFA administration showed normal appearance of the epidermal and dermal layers, highly increased and well developed hair follicles with their sebaceous glands were detected with normal distribution to some extent, of collagen fibres.
Skin tissues of rats administrated with AFA for twenty one days post-carrageenan injection and examined after five and twenty one days showed striking recovery as compared to the skin of carrageenan group only, but increased collagen fibres in the dermal layer were detected after five days while normal distribution of collagen fibres were demonstrated after twenty one days. The quantitative histochemical measurements recorded a significant increase in PAS+ve materials , total protein and amyloid β -protein in the carrageenan injected group while supplementation with AFA alone or AFA post
carrageenan injection showed a trend toward lowering incidence of skin histochemical changes induced by carrageenan injection. Skin tissues of carrageenan group showed a significant increase in mast cells count in the dermal layer after five and twenty one days post-treatment. AFA treated group exhibited non-significant increase of mast cells in the dermal layer all over the experimental periods, while rats administrated AFA post-carrageenan injection exhibited a significant increase in count of mast
cells after five days and non-significant increase after twenty one days Conclusion: using Aphanizomenon flos- aquae as a natural agent exerted a marked antiinflammatory role against the histopathological and histochemical lesions induced by carrageenan injection.

The possible anti-inflammatory role of the blue green

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Vol.2 No.6 -9 : The possible anti-inflammatory role of the blue green algae ,Aphanizomenon flos-aquae on adult male rats.

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By : Samir Atea Mohammed1, Hemmat Mansour Abdelhafez1, Fatma Ahmed Eid1 Ola Mohammed Abdel-Raouf2, Rasha Mohammed Ibrahim1

Abstract

Aphanizomenon flose-aquae (AFA) is a fresh water unicellular blue green microalgae like Spirulina, but most AFA is harvested from the wild in volcanic regions leading to high levels of trace minerals. Aphanizomenon flos- aquae have been traditionally used for over 25 years for its health-enhancing properties. To investigate the possible anti-inflammatory role of Aphanizomenon flose-aquae, rats were injected with carrageenan. Results obtained in the present study showed that injection of rats with carrageenan led to significant decreases in red blood cells (RBCs) count, hemoglobin concentration (Hb) and hematocrit value (Hct) as compared to the control group. In contrast, the levels of white blood cells (WBCs) count, alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) activities were significantly increased; this was accompanied by histopathological and histochemical changes. Meanwhile, treatment of rats with AFA for 5 and twenty one days did not exhibit a toxic effect on the liver. Also, treatment of rats with AFA postcarrageenan injection exhibited anti-inflammatory activity and improved hematological parameters and liver function profile. In conclusion, using Aphanizomenon flos- aquae as a natural agent showed a strong anti-inflammatory role.

The possible anti-inflammatory role of the blue green algae ,Aphanizomenon flos-aquae on liver
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