Vol.9 No.4-6: Detection of Candidemia in a Sample of Iraqi Neonates Admitted to the Neonates Intensive Care Unit (NICU) by Molecular Methods.

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BY: Mihad Shakir Nasif 1*, Azhar Abdul Fattah Al-Attraqchi 2, Areej Abdul Abass3

1Department of Physiology, Al-Iraqia University/ College of Medicine -Baghdad, Iraq.

2PhD in Department Microbiology Medical College/Al Nahrain University, Iraq.

3MbchB/CABP professor in pediatric medicine/pediatric department/Medical College/Al Nahrain University, Iraq.

Abstract:

Candidemia is a leading cause of sickness and mortality in neonatal care. Although current diagnostic methods are beneficial, a better knowledge of molecular pathways is necessary for enhancing detection. This study used molecular techniques to assess the incidence of candidemia in infants being treated in Iraqi NICUs. Using a cross-sectional experimental design, blood samples from newborns exposed to different risk factors were analyzed. The use of polymerase chain reaction (PCR) that targeted the ITS1 and ITS2 regions facilitated the identification of fungi. Several Cladosporium species, such as Cladosporium macrocarpum, Cladosporium allicinum, Cladosporium limoniforme, Cladosporioides, and Cladosporium tenuissimum, were found, which was unexpected. A phylogenetic study indicated the widespread distribution of these strains throughout Asia and North America. Cladosporium’s unexpected appearance necessitates a broadening of infection control measures and diagnostic perspectives in healthcare facilities. The findings of this research stress the need for constant vigilance and an all-encompassing approach to infection and diagnosis management in NICUs.

Detection-of-Candidemia-in-a-Sample-of-Iraqi-Neonates-Admitted-to-the-Neonates-Intensive-Care-Unit-NICU-by-Molecular-Methods.

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Vol.9 No.4-5:Chemical composition and antifungal properties of Oregano essential oils against the Moroccan soil-born pathogen Fusarium culmorum

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By: Jamila Bouarda1,2, Mustapha Labhilili2, Ilyass Maafa3,4, Aicha El Aissami3, MohammedBenchachoˡ and Fatiha Bentata2*

ˡ Laboratory of Botanic, Biotechnology and Plant Protection, Department of Biology, Faculty of Science University Ibn Tofail, Kenitra, Morocco

2 Research Unit of Plant Breeding and Conservation of Plant Genetic Resources, National Institute for Agricultural Research, Rabat, Morocco

3Research Center in Plant and Microbial Biotechnologies, Biodiversity and Environment, Faculty of Sciences, Mohammed V University of Rabat, Morocco

4International Center for Agricultural Research in the Dry Areas (ICARDA), Rabat 10080, Morocco

Abstract:

In our study, the application of Oregano was investigated in vitro against ten isolates of Fusarium culmorum the causative agent of wheat root rot in Morocco. The chemical composition of essential oils extracted by hydrodistillation from the aerial parts of Origanum onites and Origanum compactum was analyzed by GC. The major components of O. onites Eo were Carvacrol (29,7%) and p-cymene (22,48%) while Thymol (26,25%) was the predominant constituent in O. compactum EO. The results of the antifungal activity revealed that both oils have an inhibitory effect against all tested isolates. O. onites and O.compactum Eos inhibited the growth of all isolates tested by 100% at the concentration of 1 µL.mL−1 and 1.2 µL.mL−1 respectively. They have the potential to be used as antifungal agents for the control of wheat Rot root.

Chemical-composition-and-antifungal-properties-of-Oregano-essential-oils-against-the-Moroccan-soil-born-pathogen-Fusarium-culmorum

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Vol.9 No.4-4: MiRNA-122 association with TNF-α in some liver diseases of Egyptian patients

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By: Ahmed Abdelhalim Yameny1, Sabah Farouk Alabd1, and Magda Ahmed M. Mansor2

1Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of      Sadat City, Egypt

2Department of Histology, Faculty of Medicine, Menoufia University, Egypt

Abstract:

Background:  Due to the high frequency of HCC, ongoing research is needed to find precise, non-invasive biomarkers for early identification and follow-up that will improve prognostic results. Patients and methods: this study was conducted on 90 patients with liver diseases and 25 healthy control G1, patients divided into 4 groups, (G2) 25 patients with HCV infection, (G3) 25 HCC+HCV infection, (G4) 25 patients with HBV infection, (G5) 15 patients with HCC + HBV. Results: Serum miR-122 and TNF-α levels were increased in HCV and HBV infection significantly with p-value >0.001*compared to the control group, and their levels decreased when developed into HCC but still higher than the healthy subjects significantly with p-value >0.001. For discriminating HCV from HCV+HCC the cut-off for miR-122 was >7.1 at sensitivity 100%, specificity 100%, and the AUC was 1.0 (Excellent) P-value <0.001, also the sensitivity and specificity for TNF-α 72%, and 60% respectively with cut off >12.1 and AUC of 0.745 (Good) p-value 0.003. For discriminating HBV from HBV+HCC the cut-off for miR-122 was ≤6.4 at a sensitivity of 86.67% and specificity of 96%, and the AUC of miR-122 was 0.99 (Excellent) P-value <0.001, also the sensitivity and specificity for TNF-α 93.33%, and 48.0% respectively with cut-off ≤15.73, TNF-α has AUC of 0.527 (fair) it was not significant p-value 0.780.

MiRNA-122-association-with-TNF-α-in-some-liver-diseases-of-Egyptian-patients

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