Vol.9 No.4-3:Green Synthesis of Copper Nanoparticles Using Coffee Arabica: Larvicidal and Biochemical Study

/0 Comments/in , , /by

By: Yousef Abdulwahab1,3 , Ahyar Ahmad1,*, Isra Wahid2, Paulina Taba1

1Department of Chemistry, Faculty of Mathematics and Natural Sciences, Hasanuddin University,  Makassar, Indonesia.

2Department of Parasitology, Faculty of Medicine, Hasanuddin University,  Makassar, Indonesia.

3Department of Biology, Faculty of Applied Sciences, Taiz University, Taiz, Yemen.

Abstract

Aedes aegypti is a major problem in transmitting dangerous diseases to humans, including dengue fever. Therefore, this study aimed to synthesize copper nanoparticles from Coffee arabica and use them in the control of this dangerous insect. To confirm and characterize the formation of copper nanoparticles ultraviolet spectrophotometer, Fourier transform infrared spectrometry, and X-ray diffraction methods were used. To study the toxicity of copper nanoparticles on some biochemical parameters in the body of the insect, the amount of glucose, total protein, and metabolic enzymes such as aspartate transferase (AST), alkaline phosphatase (ALP), and lactate dehydrogenase (LDH) were analyzed. The results indicated that the highest mortality rate in Aedes aegypti larva was 96.3 % at a concentration of 100 ppm, and LC50 and LC90 were 5.7 and 36. 5%, respectively. It also showed that the lowest rate of emergence of pupae is 3.75 % at a concentration of 100 ppm, and the lowest rate of emergence of an adult insect is 2.5 % at a concentration of 50 ppm. Biochemical analyses indicate a significant decrease in the amount of total protein, alkaline phosphatase, and lactate dehydrogenase enzyme, but a significant increase in the amount of glucose and aspartate transferase enzyme was observed. Based on the obtained results, copper nanoparticles are considered a strong and promising candidate for controlling Aedes aegypti mosquito larvae and inhibiting their developmental stages.

Green-Synthesis-of-Copper-Nanoparticles-Using-Coffee-Arabica-Larvicidal-and-Biochemical-Study-1

Download PDF

Vol.9 No.4-2: Interconnection between oxidative stress and type 2 diabetes mellitus

/0 Comments/in , , /by

By: Vaishali S. Pawar1*, Ajit Sontakke2, and Satyajeet K. Pawar3

  1. MD Biochemistry, Assistant Professor, Department of Biochemistry, KVV(DU), KIMS, Karad, Maharashtra, India. 
  2. MD Biochemistry, Professor & HOD, Department of Biochemistry, KVV(DU), KIMS, Karad, Maharashtra, India.
  3. MD Microbiology, Associate Professor, Department of Microbiology, KVV(DU), KIMS, Karad, Maharashtra, India

Abstract

Total antioxidant capacity (TAC) and malondialdehyde (MDA) are two important biomarkers used in the context of diabetes mellitus (DM) to assess oxidative stress and damage. This study aimed to compare TAC and MDA levels in diabetic and non-diabetic individuals and find out the correlation between them. Estimation of TAC and MDA levels was done in a total of 200 individuals (100 non-diabetic and 100 diabetic individuals) by using standard spectrophotometric methods. This case-control study was done from May 2022 to Dec 2022 in a tertiary care hospital. For statistical analysis, version 20 of SPSS software was used. MDA and fasting plasma glucose (FPG) levels were significantly higher (P=0.000) and TAC levels were significantly lower (P=0.000) in diabetic than non-diabetic individuals. A significant negative correlation was observed between MDA and TAC in both groups. No significant correlation was found between MDA, TAC, and FPG levels. With the rise in the duration of diabetes significant increase was found in MDA and FPG levels. Also, there was a significant decrease in TAC levels. The combination of increased MDA levels, elevated FPG levels, and decreased TAC with increasing duration of diabetes indicates a state of heightened oxidative stress in DM patients.

Interconnection-between-oxidative-stress-and-type-2-diabetes-mellitus

Download PDF