Vol.9 No.4-15: Synergistic infection of Helicobacter pylori with IL-Iβ Gene Polymorphism Among the Liver Transplant Recipients

By: Mohamed Y. Nasr¹, Ghada Hassan¹_,Mohamed Ahmed Saleh², Ehsan Hassan³, Khalid Bassiouny¹, Amal Abdel-Aziz¹, Asmaa Ibrahim ^{1, 4}, Mofida A. Keshk⁵

1 Department of Molecular Biology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City (GEBRI, USC), El Sadat City, Egypt.

2 Assistant consultant internal medicine, National Hepatology and Tropical Medicine Research Institute (NHTMRI), Cairo, Egypt.

3 Pathology Department, National Hepatology and Tropical Medicine Research Institute (NHTMRI), Cairo, Egypt.

4 Departments of Medical Parasitology, Faculty of Medicine, Cairo University, (Laboratory of Molecular Medical Parasitology, LMMP).

5 Department of Molecular Diagnostics & Therapeutics, Genetic, Genetic Engineering and Biotechnology Research Institute, University of Sadat City (GEBRI, USC), El Sadat City, Egypt.

Abstract

Background and Objective: Patients receiving liver transplantation are more likely to develop a wide range of infectious complications such as Helicobacter pylori (H. pylori).This study aims to assess the prevalence of H. pylori in individuals receiving liver transplants through the lymph nodes. Moreover, to investigate the relationship between the polymorphisms in the IL-1βgene and the H. pylori infection.

Methods: A total of 43 liver-transplanted patients were selected. They performed a history interview and physical and biochemical examination. The UreA gene and the virulent gene CagA were molecularly screened from paraffin-embedded tissue, additionally, the polymorphism of IL-1β was performed by Polymerase Chain Reaction-Restriction length polymorphism (PCR-RFLP).

Results: all 23(53.5%) patients showed H. pylori infection with UreA gene detection. Out of them 8 (34.8%) were positive for the CagA virulence gene. The infected group with H.pylori showed a significant decrease in albumin (P<0.05). In the case of CagA-positive patients showed a significant decrease in albumin, urine creatinine, HB, platelets, total calcium, and AT III and an increase in urea, creatinine, uric acid, iron, INR, K, TSH, LDH, triglyceride, lipase, and amylase. Analysis of IL-1β (C+3954T), the TT genotype is considered a protective factor, while the CT genotype might be a risk factor for infection. Concerning IL-1β (T-31C) in the case of CagA positive C allele might be protective from infection with CagA-positive strain.

Conclusion: Based on these results, H. pylori appears to have a significant impact on the progression of liver transplanted patients with an interference role of IL-1β polymorphism.

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