Vol.9 No.4-10: Whole Genome Sequence and Comparative Genomics Analysis of Citrobacter Freundii Isolate from Burn Skin

BY: Namariq Al-Saadi¹, Ohood A. Radhi², Alabbas Alaa³_, Ali Adnan Hashim⁴

^1* Science Department, College of Basic Science, Misan University, Misan, Iraq.

¹Al-Manara, College for Medical Sciences, Misan, Iraq

²Department of Basic Science, College of Nursing, University of Kufa, Iraq.

³Department of Microbiology, College of Medicine, University of Karbala, Kerbala, Iraq.

⁴Al-Manara, College for Medical Sciences, Misan, Iraq

Abstract:

Background: The emergence of resistance to numerous novel antibiotics, which serve as the ultimate resort for treating infections caused by Gram-negative bacteria that are resistant to multiple drugs, poses a significant risk to public health. The Citrobacter freundii strain obtained from the burned skin of an adult exhibited resistance to a broad range of antibiotics, including colistin. The genome features that underlie the antibiotic-resistance phenotype of this isolate was characterized using whole genome sequencing (WGS). Methods: Genome sequencing on the Illumina platform was performed on the provided DNA samples (reference order number HN00194138; Psomagen/USA). The assembled genome was assembled and annotated by PATRICS. Results: The average G+C content was 55.75 percent throughout the 113 contigs in the genome. There are 9,825 protein-coding regions (CDS) in this genome, along with 149 transfer RNA (tRNA) genes and 8 rRNA genes. There were 1,584 putative proteins annotated and 8,241 proteins with known functions. PATRICS can identify 1592 hypothetical proteins. Additionally, 2132 proteins were displayed together with 8233 protein assignments under functional proteins.

Conclusion: The Iraqi multidrug-resistant C. freundii strain has not been reported before. These genetic findings help explain C. freundii’s pathogenicity. Genomic analysis has helped us comprehend the probable resistance mechanism and the isolates’ phylogenetic link with public-domain draft genomes.

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