Vol.5 No.2 – 2 : Using starchy waste as a promising raw material for bioethanol production with consequence purification using chitosan / sodium alginate polymeric membrane
By: Tarek H. Taha1*, M. A. Abu-Saied2*, Elsayed M. Elnaggar3, Ranya A. Amer4, Ahmed E. Mansy4, Gamal M. Elkady3
1Environmental Biotechnology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab, 21934, Alexandria, Egypt.
2Polymer Materials Research Department, Advanced Technology and New Materials Research Institute, City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab, 21934, Alexandria, Egypt.
3Chemistry Department, Faculty of Science, Al-Azhar University, Cairo, Egypt, 11884.
4Environment and Natural Materials Research Institute (ENMRI), City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab, 21934, Alexandria, Egypt.
Abstract
Current study is concerned by using environmental wastes as raw materials for bio-ethanol production. Alpha amylase enzyme has been used to convert the starch molecules of kitchen waste into simple units of glucose which subsequently fermented into bioethanol. The obtained results showed that 40% substrate and 100 µl of α-amylase were the optimum concentration to produce the highest glucose units at 417.9 and 482.5 milligram/deciliter (mg/dl), respectively. The highest bioethanol production of 423.5 mg/dl was obtained after anaerobic fermentation of free yeast cells at 30oC without shaking. Both of bio-ethanol and 25% ethanol were separated by using amicon cell ultra-filtration system integrated with chitosan or sodium alginate membranes under nitrogen pressure. Each membrane was characterized by Scanning Electron Microscope (SEM) and Ion Exchange Capacity (IEC); while, the hydrophilicity/hydrophobicity was investigated using contact angle. The whole system succeeded to elevate the ethanol concentration in a range of 47-50%, which could be increased with more polymeric modifications.
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