Vol.3 No.4 – 10 :Morphological and molecular characterization of some olive (Olea europaea) cultivars in El-Arish, Egypt

BY: Amina Abdel Hamid Mohamed 1*, Mohamed A. Nagaty 2,  Manal M. S. El-Baghdady¹  , Khaled H. Radwan 1, 3

1 Agricultural Genetic Engineering Research Institute, Agricultural Research Center, Giza -12619, Egypt.

2 Plant Production Department, Faculty of Environmental Agricultural Sciences, Arish University, El-Arish, North Sinai, Egypt.

³ National Biotechnology Network for Expertise- Egypt.

Abstract

Morphological characters, along with three different random PCR based markers, (ISSR, SCoT and RAMP) were used to characterize and to assess the genetic diversity among the main nine olive cultivars in El-Arish, Egypt. Analyses of 16 morphological characters revealed the existence of a recorded genetic variability among the studied cultivars. Moreover, 12 ISSR primers, 13 SCoT primers and 11 RAMP primer combinations (PCs) produced 197, 242 and 172 of total loc with 163, 236 and 140 of them being polymorphic respectively. Average polymorphism information content (PIC value) of 0.26, 0.31 and 0.26 detected for ISSR, SCoT and RAMP markers respectively.  Based on morphological and the selected molecular markers, the nine olive cultivars were grouped in two distinct clusters. The highest genetic similarity observed was that between Teffahi and Ageezi, while the least similarity was that recorded between Ageezi Shami and Koroneiki.

The tested marker systems would serve as a complementary tool to provide a more complete understanding of the diversity available in olive populations in Egypt.

Download PDF