Vol.2 No.09 -1 : Distribution of bacteria in Lake Qarun, AL Fayoum, Egypt (2014 -2015) in relation to its physical and hydrochemical characterization.

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By : Mohamed Tawfiek Shaaban1, Hassan A.H. Ibrahim2, Amer Ahmed Mohammed Hanafi3

Abstract

The bacteriological monitoring of Lake Qarun water and sediment (aerobic heterotrophs, Staphylococcus sp., Vibrio sp. Aeromonas sp., S. feacalis, E. coli, and total coliform sp.) through the period of study (2014-2015) was carried out. Six common bacterial isolates were fully identified as; Bacillus firmus, Bacillus stratosphericus, Exiguobacterium mexicanum, Stenotrophomonas rhizophila, Halomonas stevensii, and Halomonas korlensis based on partial sequencing of 16Sr DNA. In addition, physical and chemical analyses of Lake Qarun water and sediment (pH, temperature, salinity, dissolved oxygen, BOD, COD, and nutrients) were estimated. The pH varied between 7.08 at sit I and 8.73 at sit V. The temperature varied between 15.6oC at sit VIII and 32.2oC at sit I. The salinity ranged between 3.18% at sit I and 51.72% at sit III. The dissolved oxygen fluctuated between 3.89 md/l at sit VI and 8.96 md/l at sit IV. The BOD ranged between 1.08 mg/l at sit VIII and 6.47 mg/l at sit IV. The COD varied between 11.36 mg/l at sit I and 53.64 mg/l at sit VIII. The phosphate in lake water varied between 1.453.4 μg/l at sit VIII and 498.4 μg/l at sit I. The ammonia ranged between 14.73 μg/l at sit II and 1485 μg/l at sit I. The nitrite varied between 4.10 μg/l at sit IV and 646.5 μg/l at sit I. The nitrate varied between 14.79 μg/l at sit III and 2138 μg/l at sit I. The silicate fluctuated between 894.6 μg/l at sit VI and 4682 μg/l at sit VII.

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Vol.2 No.09 -2 : Effect of noise on blood pressure and heart rate for some workers in Missan governorate, Iraq.

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By : Abbas,Ch. Mraissl1, Haitham, H. Abd2, Nadhm , A. Kalf2

Abstract

The noise has become a very important (stress factor) for the human community especially in industrial cities. The aim of this study was to investigate the relationship between occupational noise level with arterial blood pressure (systolic and diastolic), heart rate , pulse pressure, mean arterial pressure for some workers from industrial region (Black smiths, Lath machine), Generators and Grinders. The data were collected between (The first of November -2015 to the Thirty of January -2016).The study group included (60) workers in various professions and exposed to the industrial noise levels with mean (97.25dB), with mean age (30.35) years and mean weight (72.46) Kg and mean height (168.86) cm. The control group consist of (30) subjects with mean age (31.45) years and mean weight (66.37) Kg and mean height(162.93)cm. taken from students and staff members of Missan university with some donors who never lived or worked in noisy environment. The results of this study showed significant changes in (P<0.05) for systolic and diastolic blood pressure, heart rate, pulse pressure , mean arterial pressure in the workers for all occupations . It is concluded from this study that the industrial noise could be causal factor for cardiovascular disturbances and increase the blood pressure and heart rate in the workers during exposure to the industrial noise.

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Vol.2 No.09 -3 : Anti-oxidant and Anti-inflammatory activity of Tabebuia rosea (Flowers).

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By : S.Solomon 1 M.M.Senthamilselvi2 N.Muruganantham3

Abstract

The aim of this study is to investigate the anti-inflammatory and anti-oxidant activities of the sample isolated from the ethyl acetate fraction of flowers of Tabebuia rosea. Anti-inflammatory activity of the sample was determined by HRBC membrane stabilization and Albumin denaturation methods. Anti-oxidant activity of the sample was determined by DPPH assay and ABTS method. The results of the study suggest that the sample isolated from the ethyl acetate fraction possesses anti-oxidant and anti-inflammatory activity.

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Vol.2 No.09 -4 : Ability of anti-SCa-1+ and CD105mesenchymal stem cells to restore fertility in induced azoospermic mice.

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By : El-Fiky, B.A.

Abstract

Impairment of spermatogenesis has been shown in patients treated with chemotherapy against different malignancies resulting in azoospermia. The present study was designed to assess the ability of anti-SCa-1and CD105 mesenchymal stem cells to restore fertility in induced azoospermic mice following cyclophosphamide administration. Forty male mice were divided randomly into four groups, each group included 10 male albino mice which has an average body weight 22-25 g; group (1): normal control, group (2): male azoospermic mice induced with cyclophosphamide, group (3): male azoospermic mice received anti-SCa-1+ stem cells into caudal vein, group (4): male azoospermic mice received CD105 MSCs into caudal vein. Androgen hormonal profile and histological assessment of testicular tissues were evaluated pre- and post-treatment with anti-Sca-1+ and mesenchymal stem cell transplantation. Results showed that male azoospermic mice treated with anti-SCa-1+ and CD105 MSCs stem cells after 12 weeks of treatment are able to improve androgen hormonal profile levels and resuming spermatogenesis as verified by histological assessment of testicular tissues and hormonal profile as compared to control groups. Also data obtained from the study showed no significant differences between anti-SCa-1+ and CD105 MSCs treated groups. In conclusion, both of anti-SCa-1 and CD105 MSCs have the potentiality to differentiate into germ cells and sperms in vivo in testicular microenvironment, and also able to restore testicular functions.

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Vol.2 No.09 -5 : Biochemical and histological studies on the possible protective impact of the herb basil (Ocimum basilicum) on adriamycin induced toxicity in rats. I. Influence on the liver.

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By : Bayomy* M.F.F., Sakr, S.A., Gendia S.E. M.

Abstract

The purpose of this work was to evaluate the effect of basil (Ocimum basilicum) against hepatotoxicity induced in albino rats by the anticancer drug adriamycin (ADR). The biochemical results showed that adriamycin caused significant elevation in serum ALT (Alanine aminotransferase) and AST (Aspartate aminotransferase) enzymes after 4, 6 and 8 weeks of treatment. It also caused an increase in malondialdehyde (lipid peroxidation marker) and decrease in activities of the antioxidant enzymes, superoxide dismutase and catalase. This drug has resulted in various histological changes in the liver. These changes include impairment of the normal structural organization of the hepatic lobules, congestion and dilatation of blood vessels, cytoplasmic vacuolization of the hepatocytes, leucocytic infiltrations and fatty infiltration. Treating animals with ADR and basil (Ocimum basilicum) led to an improvement in both biochemical and histological changes induced by ADR. There are significant decreases in ALT and AST activity. Moreover, Ocimum basilicum reduced the level of malondialdehyde and increased the activity of superoxide dismutase and catalase. In conclusion the results of the present work indicated that Ocimum basilicum had a protective effect against liver damage induced by adriamycin and this is due to antioxidant activities of some substances found in water extract of Ocimum basilicum.

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