Vol.2 No.3 -6 : Teratogenicity of sodium fluoride on newly born rats.

By : Abdelalim A. Gadallah1,2

Abstract

Fluoride (F) is widely used to sterile drinking water against bacterial infection as well as for normal cleaning of teeth. Although intake of low doses of fluoride is required to prevent dental caries, increased uptake for long time injured bone and soft tissues causes fluorosis (Susheela, 1999). The present study aims to illustrate the teratogenicity and histopathological alterations of fluoride in maternal liver, kidney and thyroid glands. Twenty virgin female and male albino rats of Wistar strain at ratio of 2 female/ 1male were kept under good ventilation with controlled conditions and excess food and water were supplied ad libitum. Pregnant rats were arranged into two groups (n= 6) including, control and fluoride-intoxicated group. Body weight, size and crown rump length of newly born rats were determined. The offspring 1-day old were sacrificed by light anesthesia with diethyl ether and immediately fixed in 10% formal saline. Alizarin red S preparation of both control, and experimental groups were made and the incidences of deformed bones were recorded. Histological preparations of maternal liver, kidney and thyroid glands were made and examined under bright field light microscopy. Experimental group exhibited disruption of the normal integrity of hepatic lobules with prominent centrilobular necrosis and dilatation of blood sinusoids. Perivascular leukocytic cell infiltration was remarked with bile duct obliteration. Also, peritubular inflammatory cellular infiltration associated with degeneration of renal tubular lining epithelial cells and reduction of their tubular lumina were also detected. Degeneration of the thyroid follicles with marked reduction and vacuolation of colloid. Few numbers of the thyroid follicles exhibited exfoliation of their lining cells within their follicular lumina. Inter-follicular hemorrhage and congested blood vessels were remarked. Fluoride-intoxication showed abortion of one /6 mothers. There were numerical decreases of offspring of fluoride-intoxicated mother . Increase average of congenital malformations was observed.

6. Teratogenicity of sodium fluoride on newly born rats.
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