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Vol.10 No.1 – 2: Physiological responses (Hsp70, Mt), Oxidative stress, toxicity impacts, and risk assessment of the biomarker (Enochrus tenuicosta) to heavy metals contamination along the Red Sea coasts- Egypt.

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Eman H. Hassan1*, Eman H. Radwan2, Gaber A. Saad3, Nessrin Kheirallah3

1Biological and Geological Science Department, Faculty of Education, Alexandria University, Alexandria, Egypt

2Zoology Department, Faculty of Science, Damanhour University, Damanhour, Egypt

3Department of Zoology, Faculty of Science, Alexandria University, Alexandria, Egypt

ABSTRACT

This study aimed to investigate the effect of heavy metals accumulation in midgut tissues of Enochrus tenuicosta beetles in the Red Sea also in water and sediments of the selected locations. Also to analyze the biochemical response and HSP70 expression. Our results demonstrated that the heavy metals and oxidative stress (MDA) concentrations in the polluted location were higher than the reference one. The response of the antioxidant defense system is significantly higher in the beetles of the reference ones. MT expression and HSP70 were much higher in the polluted beetles than in the reference ones.

Physiological responses (HSP70, Mt), Oxidative stress, toxicity impacts, and risk assessment of the biomarker (Enochrus tenuicosta) to heavy metals contamination along the Re

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Vol.4 No.3 – 3 : Nephrotoxicity associated with Orlistat in normal and obese female rats

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By : Ehab Tousson, Ahmed Massoud, Attyat Salem, Shahenda A. Fatoh

Department of Zoology, Faculty of Science, Tanta University, Tanta, Egypt.

 

Abstract

Obesity is a global health concern associated with high morbidity and mortality. Therapeutic strategies include synthetic drugs and surgery, which may entail high costs and serious complications. Orlistat is a pancreatic lipase inhibitor licensed for the treatment of obesity. The current study was carried out to elucidate the modulating effect of Orlistat against obesity induced kidney toxicity in female rats. A total of 50 female rats were divided into five groups (G1, Control; G2, Orlistat; G3, Obesity; G4, Co- treated Orlistat with Obesity; G5, Post- treated Obesity with Orlistat rat group). The current study revealed that a significant increase in serum urea, creatinine, while  a significant decrease in the levels of sodium, potassium, calcium and chloride ions levels in treated rats with Orlistat while a significant increase in serum urea, creatinine, sodium, potassium and chloride ions levels in obesity group when compared with control group. In contrast; a significant decrease in serum urea, Creatinine, sodium, potassium and chloride ions levels in treated obese rats with Orlistat when compared with obesity group.  So; Orlistat induced renal toxicity when used for treatment of obesity and self-recovered obese rats is safe and better than the use of Orlistat in treatment of obesity.

Nephrotoxicity associated with Orlistat in normal and obese female rats

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Vol.4 No.2 – 1 : Protective Effects of α-lipoic acid on Biological changes Induced by α-cypermethrin in Testis Rats

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By : A. Sedky and A. Ali

 

Abstract

α-cypermethrin is one of the most potent insecticide used worldwide.This study was planned to evaluate the possible role of α-lipoic acid in α-cypermethrin induced toxicity in rats. The treated groups were;the control, α-cypermethrin, α-lipoic acid and α-cypermethrin and α-lipoic acid groups. Our results showed that administration of α-cypermethrin caused significant decrease in RBC count, PCV and Hb content and an increase of WBC count. Also, α- cypermethrin caused significant increase in the levels of cholesterol, TGs, LDL-Cand VLDL-C, while the HDL-C was decreased.In addition, α-cypermethrin caused reduction in serum testosterone, FSH, and LH levels in intoxicated rats. Furthermore, the co-administration of α-lipoic acid mitigated the toxicity of α-cypermethrin by partially normalizing these biochemical parameters. Our results were supported by histopathological observations of testis. Our data suggest that α-lipoic acid may have a protective role against α-cypermethrin induced toxicity in rats.

cyp testis 3 corrected

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Vol3 No.1 -4 : Silymarin extract modulates toxicity, injury, oxidative stress and PCNA alternations induced by tramadol in rat liver

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By : Nadhom Abd Khalaf

Abstract

Tramadol is a synthetic opioid analgesic commonly prescribed for moderate to severe pain. This study was designed to evaluate the effects of silymarin supplementation on Tramadol induce injury, oxidative stress and PCNA expression alterations on liver in rats. For this purpose 40 male albino rats were divided into four groups and treated for 4 weeks (group 1 was control, group 2 was silymarin, group 3 was Tramadol and group 4 was Tramadol plus silymarin). The obtained results revealed that; serum GPT, GOT, ALP, GGT activities and MDA levels in liver tissues were significantly increase in rats treated with Tramadol as compared to control group while, total protein, albumin, globulin levels in serum, GSH, SOD and catalase levels in liver tissues levels were significantly decrease in Tramadol group when compared with control rats. Liver sections in rats treated with Tramadol exhibited mild positive reactions were detected for PCNA-ir, marked dilation or congestion in central veins, marked cellular infiltrations, atrophied and vacuolated hepatocytes. Treated rats with Tramadol plus silymarin succeeded to modulate these observed abnormalities resulting from Tramadol as indicated by the reduction of enzymes activity and the pronounced improvement of the investigated biochemical, antioxidant parameters, oxidative stress, hepatic injury and PCNA alterations. Further studies are needed to investigate the impacts of tramadol on human health.

2017 JBSAR

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Vol.2 No.2 -7 : Assessment of the hazard effect of an environmental pollutant, (2-OH-BDE 123) using zebrafish embryos.

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By : Nessrin A. Kheirallah1# and Tamer El-Sayed. Ali2 #

Abstract

In the recent decades, many environmental pollutants have received significant attention due to their potential ability to mimic the actions of endogenous estrogens. These pollutants are referred as environmental estrogens and are suspected of causing health effects in both humans and wildlife through disruption of the endocrine system and causing male reproductive dysfunction, thus they are classified as endocrine disrupting compounds (EDCs) such as phenolic metabolites. To monitor general toxicity of this class of EDCs, embryos of zebrafish were exposed to gradual concentrations of 2-OH-BDE 123 (hydroxy-brominated diphenyl ethers). Exposures were done by immersion of 1 hour post fertilization (hpf) zebrafish eggs to 72 hpf, nominal concentration range of 0.03 : 2.5 μM. Embryos/ larvae were assessed daily for death and structural defects. Results revealed that concentrations from 0.3 μM of such metabolites were toxic to the developing zebrafish causing serious morphological alterations and internal deformations. Both toxicity incidence and potency were correlated with the concentration applied. In conclusion, these compounds induced several teratogenic effects. More studies are required for a proper risk assessment and more attention should be given to this class of chemicals in the aquatic environment.

7. Assessment of the hazard effect of an environmental pollutant, (2-OH-BDE 123) using zebrafish embryos.

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