Posts

Vol.6 No.3 – 3: Effect of Moringa oleifera leaves extract against electromagnetic field impairments on hemoglobin and testes of rat

/0 Comments/in , , /by

By: Aida, A.A *; Aziza A. Elsaeid * Shoman, H.M.** and Ola Mohamed Awad *

* Biophysics Department, Faculty of Science, Al-Azhar University, Cairo, Egypt

** Zoology Department, Faculty of Science, Al-Azhar University, Cairo, Egypt

Abstract

The present study has revealed the effect of Moringa oleifera leaves extract against electromagnetic field induced impairments. Forty-two adult white Albino male rats, weighting 150-180 g were involved in this study and divided into six equal groups. The first group was the control while the second group was daily supplemented with 200 mg/kg Moringa oleifera leaves extract orally for six days. The third group was exposed to an electromagnetic field of 1.5 mT, 50 Hz for 9 hours. The other three groups were exposed to an electromagnetic field of 1.5 mT, 50 Hz for 9 hours, and received the Moringa oleifera leaves extract in different supplemented ways. Blood samples were collected for the absorption spectrum of hemoglobin molecules analysis and serum total testosterone was studied. Samples of testes were taken for histopathological observations. The results showed that exposure of the animals to the electromagnetic field resulted in changing the absorption spectrum of hemoglobin, a highly significant reduction in testosterone level, and degenerative changes in testes. All these induced changes were decreased in groups treated by Moringa oleifera leaves extract in all ways. So through this study, one can conclude that damages induced by exposure to the electromagnetic field can be decreased by Moringa oleifera leaves extract.

Effect of Moringa oleifera leaves extract against electromagnetic field impairments on hemoglobin and testes of rat-converted (1)

Download PDF

Vol.2 No.07 -9 : Molecular and cytochemical comparative assessment between the two food additives,sunset yellow and curcumin-induce testicular toxicity in mice.

/0 Comments/in , , /by

By : Mohamed A. Ismail

Abstract

The present work was planned to study the effects of E110 (sunset yellow) as a common synthetic in Egypt and E110 (curcumin) as a natural food-drug colorants on the testis of the male mouse. The plan of work was designed to cover six parameters: histopathological, cytochemical (involving DNA and total proteins), testis weight, sperm parameters (i.e., sperm abnormalities and sperm motility), and measuring testosterone levels in blood sera. The mice were divided into three groups, ten per each. The first group remained as controls, whilst the second orally given sunset yellow-E110 (30 mg/kg b.wt/day) as SY-group and the third one E100 ‘CU-group’ also gavage 37 mg/kg b.wt., both fed on their acceptable daily intake (ADI) dosages for 60 days. The results detected that SY revealed distinct alterations in the desired parameters, particularly histological changes in structure of seminiferous tubules such as vacuolation, necrosis and multinucleate cells. Whilst, the cytochemical DNA and proteinic profiles of the SY-treatment mice exhibited severe damage in the DNA and total protein configurations. However, such deteriorations in the spermatogenic epithelia were also approved with changes in the other criteria after administration with E110. From such alterations, the E110 recorded a highly significant increase (P< 0.0001) in the abnormalities of sperm morphology and motility. Moreover, the testosterone levels in sera of male mice indicated the significant differences among groups. The molecular protocol manifested SY (E110) - induced DNA polymorphic changes in confrontation with control by primer OPC07, whilst CU (E100) kept on the control pattern. In conclusion, the present study explored the possibility of using the applied six parameters to assessment and differentiate between the two food flavours indicating that E100 (CU) is more biosafe than the synthetic additive E110 (SY).

9_Molecular_and_cytochemical_comparative_assessment_between_the_two_food_additives_sunset_yellow_and_curcumin-induce_testicular_toxicity_in_mice

Download Issue