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Vol.2 No.12 -4 : Effects of propolis on histopathological and immunohistochemical alterations in testes of thyroidectomized rat.

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By : Ezar Hafez

Abstract

The thyroid hormones, triiodothyronine (T3) and its prohormone, thyroxine, are secreted by the thyroid gland which are responsible for the regulation of metabolism in all body tissues. This work aimed to study the histopathological, immunohistochemical and biochemical changes in the adult rat testes after thyroidectomy, in addition to the role of propolis in reducing these changes. Twenty five male Wistar rats were assigned randomly into equal (5) groups; control, sham operated, shamed propolis, thyroidectomized, thyroidectomy treated with propolis. Levels in compare to control the thyroidectomized rats showed significantly decrease in serum T3 and increase in TSH levels. Degeneration and increase in the seminiferous tubules lumen also noticed. Immunohistochemical results showed a remarkable reduction of proliferating cell nuclear antigen (PCNA) and Ki-67 in spermatogonia of thyroidectomized rat testes as compared with control group. The results revealed that, propolis has an ameroliating role on thyroidectomized rats testes by decreasing damage.

4_Effects_of_propolis_on_histopathological_and_immunohistochemical_alterations_in_testes_of_thyroidectomized_rat

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Vol.2 No.6 -10 : The radioprotective role of Aphanizomenon flos-aquae (AFA) on testis of adult male albino rats

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By : Fatma Ahmed Eid , Hemmat Mansour Abdelhafez ,Samir Attia Zahkouk1 and Heba Ahmed

Mohamed Kandeal

Abstract

This work aimed to study the biochemical, histopathological and histochemical changes in the testes of
male albino rats post exposure to 4 Gy of gamma radiation and the possible radioprotective role of Aphanizomenon flos-aquae (AFA). Aphanizomenon flos-aquae (AFA) is a blue-green microalgal species which has antioxidant properties. The current experiment was carried out on 48 adult male albino rats (Rattus rattus). Rats were randomly and equally categorized into four groups: 1) Group C: control rats left without treatment; 2) Group R: rats were exposed to 4Gy of gamma-radiation as a single dose; 3)
Group AFA: rats were terated orally with 94.5mg/kg body weight/ day AFA for 3 weeks and 4) Group AFA+R: rats were administrated AFA for a period of one week before and three weeks after irradiation. Results obtained in the present study showed that exposed rats showed a significant increase in MDA in the testes, but decreased testosterone level was detected versus the control. Many histopathological lesions were observed in the testes tissue such as disturbed spermatogenic layers with vacuolated spermatogenic cells, presence of polynucleated cells, absence of mature sperms, oedema in the interstitial spaces, congested testicular arteries with thickened, dilated and corrugated walls of them, they also contained hemolysed blood cells with highly reduced, atrophied and distorted Leydig cells in the interstitial spaces. Irradiated groups showed highly increased collagen fibres under the testicular capsule, basement membranes, Leydig cells and around the blood vessels with signs of fibrosis in the capsule and some seminiferous tubules In addition, irradiated group induced a significant increase in amyloid - protein, while a significant decrease in PAS+ve materials, total protein and total DNA content was detected. AFA administration ameliorated the damaging effects of testes of radiation exposed rats. Conclusion: according to the results obtained in the current study using Aphanizomenon flos- aquae as a natural agent showed a strong radioprotective role. Key words. Gamma rays, ionizing radiation, Aphanizomenon flos-aquae (AFA), testes, rats.

Vol.2-No.6-10-The-radioprotective-role-of-Aphanizomenon-flos-aquae-AFA-on-testis-of-adult-male-albino-rats

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Vol.2 No.2 -3 : Ameliorating effect of propolis extract against equigan induced.

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By : Ahmed Massouda, Ehab Toussona, Nahla Zedanb and Ali E. Abd elwahaba

Abstract

Equigan is an androgenic steroid that improves the growth and food conversion in meat producing animals. The present study was performed to determine the ameliorating effect of propolis in the toxicity of the rat testes induced with Equigan. Fourty male albino rat were divided into four groups (10 animals each); the control group includes animals that injected intramuscularly with olive oil. The second group includes rats received propolis. The third group is the experimental group included animals that received intramuscular injections of Equigan. The last group was co-administrated group where rats received Equigan along with propolis. The results suggest that misuse of growth promoter Equigan may contribute to continuous damage of the testicular function and structure that shown a significant increase in nitric oxide (NO), malondialdehyde (MDA), testosterone, follicle stimulating hormone (FSH) and luteinizing hormone (LH) in Equigan group when compared to the control group, these results decrease in co-administrated Equigan with propolis. On the other hand a significant decrease in superoxide dismutase (SOD), catalase, Glutathione (GSH) and total thiol in Equigan group when compared with control group, and a significant increase in co-administrated Equigan with propolis. It was concluded that; propolis has ameliorating role on the biochemical alterations in Equigan induced testicular toxicity in male rat.

3. Ameliorating effect of propolis extract against equigan induced.

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Vol.1 No.5 -5 : Apoptotic Marker Alternations in the Spleen of Experimentally Hyperthyroid and Hypothyroid Rat.

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By : Ezar Hafez; Ahmed Masoud; Magdy Barnous; Ehab Tousson

Abstract

Apoptosis plays a critical role in the development and homeostasis of multicellular organisms, especially those with high cell turnover such as the lymphoid system. The current study aimed to examined the effects of changes in thyroid hormones on apoptosis of spleen in male rats. 30 rats were equally divided into three groups (10 animals each). G1, control group in which animals did not received any treatment; G2, Hypothyroid group in which rats received 0.05% 6-n-propyl-2-thiouracil (PTU) in drinking water for 6 weeks; G3, Hyperthyroid group in which rats received 100 μg/Kg L-Thyroxin sodium administration in drinking water for 6 weeks. In the present study; serum T3 and T4 concentrations were depressed and serum TSH concentration was significantly elevated in rats receiving PTU-induced hypothyroidism. On the other hand; serum T3 and T4 concentrations were significantly elevated and serum TSH concentration was depressed in rats receiving L-Thyroxin sodium-induced hyperthyroidism. In the current study; spleen in both hypothyroid and hyperthyroid rats revealed many of abnormalities as marked disruption of spleen structure, loss in distinction between the white and red pulps, degeneration and vacuolation with an increased in the lymphocyte population. Also, a significant increase in p53 and Caspase3 apoptotic cells and a significant decrease in Bcl-2 antiapoptotic cells in the spleen tissues revealed the possibility of the apoptosis occurrence after PTU or Thyroxin administration in the case of hypothyroidism and hyperthyroidism.

5. Apoptotic Marker Alternations in the Spleen of Experimentally Hyperthyroid and Hypothyroid Rat.

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