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Vol.7 No.4 – 3:Molecular and Biochemical Studies on Some Natural Compounds and Their Effect on the Streptozotocin-induced Diabetic Rats and Their Role in Treatment

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By: Adel Abaskharon Guirgis1, Dawoud Fakhry Habib2, Hanan Hanna3 and Ramy Abosaif Mahmoud1

1Molecular Biology Department, Genetic Engineering &Biotechnology Research Institute University of Sadat City, Egypt

2 Clinical Biochemistry Department, National Research Centre (NRC), Egypt

3 Chemical Pathology Department, Faculty of Physical Therapy, Pharos University in Alexandria, Egypt

Abstract

Background: The number of individuals with diabetes mellitus (DM) worldwide has more than doubled over the past three decades, and it has been predicted that the number of diabetic patients would increase to 439 million by 2030, so many efforts are being made to find a new and effective treatment for diabetes mellitus.

Objective:   This work aims to study the biochemical and molecular effect of the Moringa oleifera MO and Ficus sycomorus FLE in streptozotocin-induced diabetic rats and compare them with the effect of metformin, by estimation of the expression of β-actin and glucose transporter GLUT2, GLUT4, and Insulin Receptors genes in studied groups, Determination of fasting blood glucose and insulin levels before and after induction of (STZ), Quantitative estimation serum cholesterol, TG levels, HDL, LDL, Some antioxidant enzyme activity Glutathione peroxidase, catalase and lipid peroxidation in plasma. Results: MO and FLEshowed promising anti-diabetic potential in diabetic-bearing albino mice which can be attributed to its anti-inflammatory effect. This could serve as a stepping stone towards the discovery of newer safe and effective anti-diabetic treatment.

Molecular and Biochemical Studies on Some Natural Compounds and Their Effect on the Streptozotocin induced Diabetic Rats and Their Role in Treatment-converted

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Vol.2 No.11 -2 : Efficacy of saffron extract and fenugreek seeds supplementation on liver of streptozotocin induced diabetic rats

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By : Doaa A. Ali , Hanaa Serag , Ahmed Abdeen and Radwa Refaat

Abstract

The present study aimed to investigate the possible hypoglycemic and hepatoprotective effects of saffron extract (100mg/kg b.wt) and fenugreek supplementation (1.4 g/day/kg b.wt) in STZ- induced diabetic rats at biochemical, histological, histochemical and ultrastructural levels. The results indicated that a single dose of STZ (50 mg /kg b wt, i.p) caused a significant increase in serum and liver total lipids, total cholesterol and triglyceride as well as serum low density and a very low density lipoprotein, accompanied by an increase in serum glucose level and oxidative stress marker, malondialdehyde and hydrogen peroxide level as well as serum aspartate and alanine aminotransferase activities. The results also reported a significant decrease in the high density lipoprotein, serum and liver total protein, serum albumin and globulin contents and A/G ratio, as well as a significant decrease in serum insulin level and the liver enzyme activities and the hepatic glutathione content. Histologically, STZ treated group showed degenerative changes in the pancreas and alteration in the structural integrity of the hepatocytes and their intracellular organelles. Histochemically, liver sections of STZ treated animals displayed marked decrease in the glycogen and total protein contents. On the other hand, the intake of saffron or fenugreek minimize the disturbance observed in the most tested parameters resulted from STZ administration and improve the liver structure and functions. Finally, it can be concluded that the intake of natural product such as saffron or fenugreek may be effective in reducing the diabetic liver injury induced by streptozotocin and the fenugreek supplementation showed more amelioration than saffron extract.

2-Efficacy-of-saffron-extract-and-fenugreek-seeds-supplementation-on-liver-of-streptozotocin-induced-diabetic-rats
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