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Vol.9 No.3-4:Relationship between LSP1 polymorphisms and the susceptibility to chronic kidney disease with hypertensive

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By: Ahmed MudherAL khaykanee1,Thulfeqar Ahmed Hamza2, Soura Alaa Hussein3

1 General Directorate of Education of Babylon Governorate, Iraq

2 Chemistry Department- College of Science for Women – University of Babylon, Iraq

3Al-Rafidain University College, Iraq

Abstract

Background: LSP1 gene polymorphisms have been tied with some diseases as well as some types of cancer.      Aims: aimed to Evaluate the link of LSP1gen  rs569550 andrs592373 linked with CKD with hypertension. Methods: 100 patients with CKD without hypertension, 100 patients with CKD with hypertension, and 100 controls were genotyped for LSP1gen  rs569550 andrs592373 using allele-specific Real-Time PCR analysis.

Results: genotype TT and GT of the LSP1 rs569550 were associated with a significantly lower risk of CKD with hypertension in patients with CKD without hypertensive  [OR (95 % CI) = 0.2 (0.08 – 0.4), P < 0.001and[OR (95 % CI) = 0.38 (0.28 – 1.23), P < 0.001*]. Patients less likely to be affected were carriers of the allele T . CKD with hypertensive than those they have the G allele [OR (95 % CI) = 0.43 (0.29 – 0.67), P < 0.001*].

Related LSP1rs592373  the variant genotypes, TC and CC were significantly associated with increased risk of CKD with hypertensive [OR (95 % CI) =4.01 (1.98 – 6.5), P < 0.001 and OR (95 % CI) =8.7 (3.01 –25.65), p <0.001 respectively]. Similar trends were observed at the allele levels, carriers of the C allele were at a higher risk for developing CKD with hypertensive [OR (95 % CI) = 3.02 (2.05 – 3.98), p< 0.001].

Conclusions: LSP1 rs569550 and rs592373 genes were associated with CKD with hypertensive sensitivity suggesting its inclusion in CKD with hypertensive in CKD without hypertensive patients.

Relationship-between-LSP1-polymorphisms-and-the-susceptibility-to-chronic-kidney-disease-with-hypertensive

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Vol.6 No.1 – 1: A pilot study of the paraoxonase-1 (Q192 R) gene polymorphism association with prostate cancer in the Egyptian population

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By: Hany Abd Al Hamid1, Mohamed Y. Nasr1, Asmaa Ibrahim1,3, Khalid Bassiouny1,  Manal Mohamed Abd Al Aziz2

1. Genetic Engineering and Biotechnology Research Institute, University of Sadat City (GEBRI, USC), Egypt

2. Clinical Pathology Department, Faculty of Medicine, Ain shams University, Cairo, Egypt

3.Diagnostic and Research Unit of Parasitic Diseases (DRUP), Department of Medical Parasitology, Kasr Al-Ainy Faculty of Medicine, Cairo University, Cairo, Egypt

Abstract

Background and Objectives: Prostate cancer (PCa) is one of the most common cancer types in men and recognized as the fifth cause of death globally. Human paraoxanase-1 (PON1) is an enzyme synthesized in the liver and linked with high-density lipoprotein (HDL). The current study aimed to evaluate the association between PCa and PON1 enzyme polymorphism in Egyptian patients.

Subjects and methods: A total of 100 persons. Fifty patients diagnosed as PCa patients (group I) along with fifty non-PCa (group II) of matchable age were enrolled in this study. Patients with another type of cancer or any chronic disease were excluded. Prostate-specific antigen (PSA) was assayed in all subject’s study by using ELISA Kit Protocol (Cat. No.: EK-310-19); in addition to CBC, hematological parameters (hemoglobin, total leucocyte count and platelets count) were estimated by using automated hematology analyzer (Pentra 80) according to manufacturer’s instructions. PON1 Q192R gene polymorphism was performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. Results: PON1 (Q192R) gene polymorphism showed QR genotype in PCa patients (group I) ]32(64%)[ compared with none-PCa (group II)] 4(8%)[. The association between PON1 (Q192R) gene polymorphism with prostate cancer group and PSA were statistically significant (P value= 0.000). Hemoglobin showed significant association with PCa patients (group I) and PSA (P = 0.000), while total leucocyte count and platelets count showed non-significant when compared with the control groupConclusion: Such findings showed that PON1 (Q192R) gene polymorphism could be associated with prostate cancer and disease growth. Recommendation, furthermore comprehensive stud­ies are required to confirm the role of PON1 gene polymorphism in the etiology of PCa.

A pilot study of the paraoxonase-1 (Q192 R) gene polymorphism association with prostate cancer in Egyptian population-converted

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Vol.4 No.4 – 10 : Breast cancer risk associated with genotype polymorphism of COMT gene in young women

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By : Abbas Ch. Mraissl

Pathology /Biology Department ,Education College , Missan University, Iraq

 

Abstract

O- methelation mediated by COMT enzyme  is an important mechanism for in activating Catechole Estrone (CE) which including 2- HE and 4- HE and transform it to 2-ME and 4-ME which act as anti- tumor genesis . Aim of this study was investigation  the association between the polymorphism in COMT genotype and the breast cancer risk in (40) young  patient’s women aged between (20-39) years were diagnosed and confirmed with breast carcinoma at AL- Sadder hospital in Missan, and (40) healthy control women aged between (18-39) years in period  between     September -2009 to April -2010 Odds ratios(OR) and confidence interval (CI) were calculated in level significant   P < 0.05. The statistical analysis showed no association between the breast cancer risk in young women and homozygous wild (Met/Met) genotype with an OR of 0.63(95%CI= 0.248 – 0.552 ), also with heterozygous  (Val/Met) genotype with an OR of 0.93 (95%CI= 0.155 – 0.44  ), and when a combination (Met/Met + Met/Val) genotype with an OR of 0.78(95%CI= 0.559-0.841), compared to homozygous mutant  (Val/Val) genotype. No significant differences in frequency of low activity alleles between cases and controls, indicating the polymorphism as a single factor may not contribute to breast carcinogenesis in young women.

Breast cancer risk associated with genotype polymorphism of COMT gene in young women-converted

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