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Vol.2 No.12 -4 : Effects of propolis on histopathological and immunohistochemical alterations in testes of thyroidectomized rat.

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By : Ezar Hafez

Abstract

The thyroid hormones, triiodothyronine (T3) and its prohormone, thyroxine, are secreted by the thyroid gland which are responsible for the regulation of metabolism in all body tissues. This work aimed to study the histopathological, immunohistochemical and biochemical changes in the adult rat testes after thyroidectomy, in addition to the role of propolis in reducing these changes. Twenty five male Wistar rats were assigned randomly into equal (5) groups; control, sham operated, shamed propolis, thyroidectomized, thyroidectomy treated with propolis. Levels in compare to control the thyroidectomized rats showed significantly decrease in serum T3 and increase in TSH levels. Degeneration and increase in the seminiferous tubules lumen also noticed. Immunohistochemical results showed a remarkable reduction of proliferating cell nuclear antigen (PCNA) and Ki-67 in spermatogonia of thyroidectomized rat testes as compared with control group. The results revealed that, propolis has an ameroliating role on thyroidectomized rats testes by decreasing damage.

4_Effects_of_propolis_on_histopathological_and_immunohistochemical_alterations_in_testes_of_thyroidectomized_rat
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Vol.2 No.5 -7 : Folic acid ameliorates L-thyroxin induced hepatotoxicity and oxidative stress in albino rats.

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By : Somya Y. Shalaby¹, Saber A. Sakr¹, Ehab Tousson², Mohamed Rabea¹

Abstract

Thyroid hormones have been known to regulate the energy metabolism of most tissues including liver. Alterations in their normal levels cause some biochemical and clinical abnormalities such as hypothyroidism and hyperthyroidism. The present study evaluated the effect of thyroid hormone, L-thyroxin on liver of albino rats. Additionally the ameliorating role of folic acid supplementation was investigated. Fifty male albino rats were randomly divided into five groups (group I, control; group II, folic acid; group III, L-thyroxin sodium administration (100 μg/kg / body weight); group IV, L-thyroxin and folic acid group and V, recovery group). The results showed that there were a significant increase in ALT, AST, MDA and nitric oxide in L-thyroxin treated rats as compared to control group. On the other hand, a significant decrease in glutathione (GSH) in L-thyroxin treated rats as compared to control group. Histological results showed that liver sections of L-thyroxin group showed histopathological lesions such as leucocytic infiltrations, congestion of central and portal veins and cytoplasmic vacuolation of hepatocytes with the presence of pyknotic nuclei, in addition to fatty infiltration. Immunohistochemical results revealed that strong positive expression of PCNA, P53, and Bcl-2 were detected in the liver section in L-thyroxin treated rats and recovered rats as compared to control and folic acid groups. However; mild to moderate positive expressions of PCNA, P53, and Bcl-2 were observed in rats treated with L-thyroxin and folic acid in liver section. This reflects oxidative stress associated with hyperthyroid state.

7. Folic acid ameliorates L-thyroxin induced hepatotoxicity and oxidative stress in albino rats.
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