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Vol.2 No.1 -4 : Ameliorating effect of propolis and moringa extract against equigan induced neurotoxicity and oxidative stress on rat hippocampus.

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By : Ehab Tousson, Ezar Hafez, Ahmed Massoud and Ahmed A. Elfeky

Abstract

In most countries worldwide, Equigan is anabolic steroid that is forbidden for human uses or for meat production. The present study was designed to investigate the ameliorating role of Propolis and moringa leaf extract (MLE) against Equigan induced neurotoxicity and oxidative stress on rat hippocampus. A total of 48 healthy male albino rats weighing 170-200 g and of 10-12 weeks age were divided into 6 groups (8 animals each). 1st Control group includes animals that injected intramuscularly with olive oil for 12 weeks. 2nd and 3rd groups were rats received Propolis and MLE respectively. 4th experimental group include animals that receive intramuscular injections of Equigan for 12 weeks; 5th and 6th groups where rats intramuscular injected with Equigan and treated with Propolis and MLE at the same time respectively. The obtained results indicate that hippocampal nitric oxide (NO), malondialdehyde (MDA), acetylcholine esterase (AChE) and total protein were significantly increased in Equigan group when compared with control group. In contrast hippocampal catalase, SOD and total thiol levels were significantly decreased in Equigan group when compared with control group. On the other hand hippocampal NO, MDA, ACE and total protein in Equigan group were significantly increased when compared with co-treated Equigan with MLE or Propolis groups. In contrast; hippocampal catalase, SOD and total thiol levels were significantly decreased in Equigan group when compared with co-treated Equigan with MLE or propolis groups. We can concluded that; MLE and propolis extract ameliorate the neurotoxicity and oxidative stress on rat hippocampus induces by Equigan.

4. Ameliorating effect of propolis and moringa extract against equigan induced neurotoxicity and oxidative stress on rat hippocampus.
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Vol.1 No.6 -4 : Effects of L-carnitine and Ginkgo bilobaon cerebral cortex in experimentally-induced epileptic seizures disease in rat

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By : EhabTousson1;Mohamed F.F Bayomy2and BasiounyFouad El-sendiony٭2

Abstract

Pentylenetetrazole (PTZ) kindling is an acknowledged model for epilepsy and refers to a phenomenon in which repeated injection of this drug causes gradual seizure. The current study aimed to determine the possible protective and ameliorative effects of L-carnitine and Ginkgo bilobaleaf extracts (GLE) against PTZinduced epileptic seizures disease in rats. A total of 80 male albino rats were equally divided into eight groups; 1st group was the control; 2nd and 3rd wereGLE and L-carnitine groups, respectivel; while the 4th group was pentylenetetrazolerat groupand the 5th and 6th groups were pre GLE and L-carnitine groups respectively; the 7th and 8th groups were post GLE and Lcarnitine, respectively. PTZ leads to an increase in malondialdehyde (MDA), glutamate (Glu) and to a decrease in catalase, super oxide dismutase (SOD), glutathione peroxidase (GPx) activity, glutathione reductase(GSH), gamma amino butyric acid (GABA) and acetylcholine esterase (AChE) in the cerebral cortex of rats when compared to control group. Pre- and post treatment with GLE and L-carnitine were improved the biochemicalalterations in cerebral cortex that treated with PTZ.

4. . Effects of L-carnitine and Ginkgo bilobaon cerebral cortex in experimentally-induced epileptic seizures disease in rat.
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Vol.1 No.1 -1 : Potentiation of antifibrotic activity of Atacand using grape seed extract (Gervital) in male albino rats

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By : Faten R. Abdel- Ghaffar1, Ibrahim A. El Elaimy1, Azza M. Mohamed2 and Mona EL. Ghreeb

Abstract

Carbon tetrachloride (CCl4) is a known potent hepatotoxic agent. The present investigation clarified the ameliorative effects of Atacand and Gervital on CCl hepatotoxicity. 24 male albino rats were divided into 4 groups, 6 rats each. Group I, served as normal control. Group II, animals were injected subcutaneously with CCl (1ml/kg b.w.) twice a week for 90 days. Group III, rats were injected with CCl4, as Group II, then treated orally with Atacand (8mg/kg b.w.), daily, for 30 days. rats were injected with the same dose of CCl then treated with Atacand (8mg/kg b.w.) along with Gervital (100mg/kg b.w.), orally, daily for 30 days. treatment showed a marked deterioration of liver function as a high increase in the levels of serum AST and ALT (153.8% and 157.8%, respectively). Also a significant decrease in liver antioxidant machinery was represented by reduced glutathione (GSH, 68.1%), glutathione transferase (GST, 69.9%) and glutathione reductase (GR, 65.65%). On the other side, the levels of liver protein carbonyl groups (PCO) and lipid peroxidation (LPO) were elevated 439.6% and 258.7%, respectively, compared to their control groups. These results indicated th damage as a result of CCl4-induced liver fibrosis. Both treatments with Atacand or Atacand + Gervital showed significant ameliorative effect for both liver and serum CCl4-induced alterations. The current study recorded the treatment with Gervital along with Atacand antifibrotic effectiveness than that of Atacand Gervital acted as a potentiator for antifibrotic activity of Atacand.

1. Potentiation of antifibrotic activity of Atacand using grape seed extract (Gervital) in male albino rats
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