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Vol.1 No.6 -8 : Acrylamide induced histochemical and immunohistochemical alterations in rat kidney cortex

By : Safia Mohamed Hassan1 and Eman Ahmed Youssef 2

Abstract

Acrylamide represents an industrial chemical and has become one of the main public health concerns since it was detected in extensively consumed food items. The present study was planned to investigate the effects of two doses of acrylamide on some enzyme activities and immunoreactivity of some immunohistochemical parameters in kidney cortex of male rats. Rats were randomly divided equally into three groups. Group (I) was control, group (II) was given acrylamide for 6 months orally in a dose of 0.05 mg/ /Kg dissolved in water for 3 doses per week and group (III) was administered acrylamide in a dose of 0.5 mg by the same way as in group II. At the end of the experiment all animals were sacrificed under anesthesia, kidney was immediately removed and processed for histochemical and immunohistochemical studies. The results revealed that the activities of lactic dehydrogenase (LDH) and xanthine oxidoreductase (XOR) were significantly increased, while succinic dehydrogenase (SDH) activity was decreased significantly compared to control group. On the other hand, immunohistochemical results showed that acrylamide significantly reduced the immunoreactions of endothelial nitric oxide synthase (e- NOS) in addition to significant increase in immunoreactivity of inducible nitric oxide synthase (i- NOS) and insignificant increase in alpha smooth muscle actin (α-SMA) with respect to control group. Over all, these results suggested that the deleterious effect in kidney tissue resulted from oral administration of acrylamide, most probably due to oxidative stress and lipid peroxidation.


8. Acrylamide induced histochemical and immunohistochemical alterations in rat kidney cortex
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Vol.1 No.5 -2 : Efficacy of a novel water soluble curcumin derivative versus Tadalafile in mediating erectile function.

By : Zaahkouk,SA.M.1, Atta H.M2., Fouad Hanan. 2, Hesham G.A1. Yehia M. Hussein.1

Abstract

The present study was conducted to assess the efficacy of a novel curcumin derivative (NCD) versus Tadalafile in erectile signaling. The study was conducted on 15 control male rats and 75 diabetic male rats divided into the following groups:, diabetic, curcumin, NCD, Tadalafile and NCD combined with Tadalafile . Cavernous tissue gene expression levels of heme oxygenase-1 (HO-1), Nrf2, NFК-β, and p38, enzyme activities of heme oxygenase (HO) and nitric oxide synthase (NOS), cGMP and intracavernosal pressure (ICP) were assessed. Results showed that 12 weeks after induction of diabetes, erectile dysfunction (ED) was confirmed by the significant decrease in ICP, a significant decrease in cGMP, NOS, HO enzyme activities, a significant decrease in HO-1 gene and a significant elevation of NFК-β, p38 genes. Administration of all therapeutic interventions led to a significant elevation in ICP, cGMP levels, a significant increase in HO-1 and NOS enzymes, a significant increase in HO-1, and Nrf2 gene expression, and a significant decrease in NFК-β, p38 gene expression. NCD or its combination with Tadalafile showed significant superiority and more prolonged duration of action. In conclusion, NCD could enhance erectile function with more efficacy and more prolonged duration of action.


2. Efficacy of a novel water soluble curcumin derivative versus Tadalafile in mediating erectile function.
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