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Vol.6 No.2 – 1: Elevated serum NAGAL levels were associated with cardiovascular diseases in pediatric chronic kidney disease

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By: Mohamed A. Abdelrazek 1,2*, Hossam T. ElAgamy 3, Lamiaa A. Barakat 3, Gamal T. Soliman4, Mohamed A. Basuni 5

1 Biotechnology Research Center, New Damietta, Egypt

2 Sherbin Central Hospital, Ad Daqahliyah, Ministry of Health, Egypt

3 Faculty of Science, Port-Said University, Port-Said, Egypt

4 Faculty of Medicine, El-Minia University, El-Minia, Egypt

5 Mansoura Children hospital, Mansoura University, Mansoura, Egypt

Abstract

Background: Early cardiovascular disease (CVD) management and prediction have become mandatory in children with chronic kidney disease (CKD). Association between neutrophil gelatinase-associated lipocalin (NGAL) and CVD events in pediatric CKD patients remains unclear. Thus, we aimed to evaluate this relationship and to clarify the association between NGAL serum levels and some CVD related parameters. Subjects and methods: A total of 70 children patients with CKD (30 with and 40 without CVD). The patient’s data were retrospectively recorded from the medical files of each patient. NGAL serum levels were measured by ELISA commercial kits. Association between different parameters was assessed by the Pearson correlation coefficient. Results: NGAL serum levels were significantly (P<0.0001) higher in patients with CVD (2450 (1335-2880) pg/mL) than patients without CVD (371 (285-1363) pg/mL) and healthy controls (295 (166-357) pg/mL). At 1300 pg/mL, NGAL has a good CVD predictive function with a high area under curve (AUC=0.871). The sensitivity, specificity, positive predictive value, negative predictive value, and accuracy were 80, 80, 63.2, 90.3 and 80%, respectively. CVD risk increase with elevated NGAL serum levels (>1300 pg/mL) (OR=2.57, 95 CI (1.62-4.09), P<0.0001). Carotid intima thickness was associated with elevated NGAL levels. Both NGAL and carotid intima thickness were significantly correlated with dialysis duration, uric acid, and lipid profile. Conclusion: NGAL was associated with CVD events in children with CKD with good predictive value supporting NGAL putative role in CVD pathophysiology. But NGAL in CVD is still in the early stages and future studied needed to evaluate its association with CVD severity.

Elevated serum NAGAL levels were associated with cardiovascular diseases in pediatric chronic kidney disease-converted

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Vol.5 No.1 – 5 : Neutrophil gelatinase-associated lipocalin and oxidative stress markers based -scores to improve the diagnostic accuracy of chronic kidney diseases

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By : Toson el-SA1 , Waly S1, Omran MM2,*

1Chemistry Department, Faculty of Science, Damietta University, Damietta, Egypt
2Chemistry Department, Faculty of Science, Helwan University, Cairo, Egypt

Abstract

Background: Biomarkers can detect chronic kidney disease (CKD) in an early stage. Patients and methods: 75 individuals suffering from chronic kidney disorders were included in this study. Serum Neutrophil gelatinase-associated lipocalin (NGAL) was assayed using ELISA. Nitric oxide (NO) in addition to total antioxidant capacity (TAC) was colormetrically measured. Results: NGAL was the most efficient marker among others. Its area under receiver operating characteristic (ROC) were 0.91 and 0.80 for differentiating patients with CKD from control individuals and discriminating patients in late stages from those in early stages, respectively. The stepwise multivariate discriminant analysis (MDA) selected two novel indexes. The first is to differentiate patients in late stages from those in early stages of CKD; namely NGAL- Total antioxidant capacity (NT) score. The second is to differentiate all of these patients from those of the control subjects; namely NGAL- nitric oxide (NN) score. The AUCs of NT and NN score were 0. 84 and 0.93; respectively. Conclusion: NT and NN scores may add more in evaluation and prediction of the progression of CKD.

Neutrophil gelatinase-associated lipocalin and oxidative stress markers based -scores to improve the diagnostic accuracy of chronic kidney diseases-converted

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