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Vol.9 No.4-13: Treatment with Leiurus quinquestratus scorpion venom ameliorates the histopathological changes of type-2 diabetic rats’ splenic tissues.

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By: Wesam Mohamed Salama*, Sabry Ali El-Naggar, Ghada Abd-Elhamid Tabl, Lamiaa Mohamed Mahmoud El Shefiey, Nabila Ibrahim El-Desouki

Department of Zoology, Faculty of Science, Tanta University, Tanta 31527, Egypt

Abstract

Type 2 diabetes mellitus (T2-DM) is a chronic metabolic disorder characterized by tissue resistance to insulin action. Leiurus quinquestriatus venom (LQV) contains a variety of bioactive components that can be used for the treatment of various diseases. This study aimed to determine the ameliorative role of LQV on the histopathological changes in splenic tissues of T2-DM rats. Forty male Sprague Dawley rats were divided into 4 groups (n = 10) as follows; Gp1 (Control group) was fed on a normal balanced diet. Gp2 (Diabetic group) was fed on a high-fat diet (HFD) for 12 weeks and then injected with streptozotocin (STZ) (30 mg/kg) i.p. Diabetic Gp3 and Gp4 groups had been injected with metformin (Met) (150 mg/kg) or LQV (1/10 of LD50), respectively. After two months of treatment, the total body weight relative splenic weight changes, and blood glucose. Also, histopathological and immunohistochemical investigations (CD-3 immunoreactive antibody) in splenic tissues were examined. The results showed that induction of T2-DM in rats led to a significant decrease in the total body weight (-38.56%), relative spleen weight (0.23 ± 0.03), and increase in the level of blood glucose (382.56 ± 2.77 mg/dL). In addition, several histopathological and immunohistochemical changes were observed in splenic diabetic tissues. The treatment of T2-DM rats with LQV led to an improvement in the total body weight of rats (4.07%), relative spleen weight (0.40 ± 0.02), decrease in the blood glucose levels (115.47 ± 1.07 mg/dL), ameliorated the histopathological and immunohistochemical changes occurred in splenic tissues of T2-DM rats.

Treatment-with-Leiurus-quinquestratus-scorpion-venom-ameliorates-the-histopathological-changes-of-type-2-diabetic-rats-splenic-tissues

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Vol.7 No.4 – 3:Molecular and Biochemical Studies on Some Natural Compounds and Their Effect on the Streptozotocin-induced Diabetic Rats and Their Role in Treatment

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By: Adel Abaskharon Guirgis1, Dawoud Fakhry Habib2, Hanan Hanna3 and Ramy Abosaif Mahmoud1

1Molecular Biology Department, Genetic Engineering &Biotechnology Research Institute University of Sadat City, Egypt

2 Clinical Biochemistry Department, National Research Centre (NRC), Egypt

3 Chemical Pathology Department, Faculty of Physical Therapy, Pharos University in Alexandria, Egypt

Abstract

Background: The number of individuals with diabetes mellitus (DM) worldwide has more than doubled over the past three decades, and it has been predicted that the number of diabetic patients would increase to 439 million by 2030, so many efforts are being made to find a new and effective treatment for diabetes mellitus.

Objective:   This work aims to study the biochemical and molecular effect of the Moringa oleifera MO and Ficus sycomorus FLE in streptozotocin-induced diabetic rats and compare them with the effect of metformin, by estimation of the expression of β-actin and glucose transporter GLUT2, GLUT4, and Insulin Receptors genes in studied groups, Determination of fasting blood glucose and insulin levels before and after induction of (STZ), Quantitative estimation serum cholesterol, TG levels, HDL, LDL, Some antioxidant enzyme activity Glutathione peroxidase, catalase and lipid peroxidation in plasma. Results: MO and FLEshowed promising anti-diabetic potential in diabetic-bearing albino mice which can be attributed to its anti-inflammatory effect. This could serve as a stepping stone towards the discovery of newer safe and effective anti-diabetic treatment.

Molecular and Biochemical Studies on Some Natural Compounds and Their Effect on the Streptozotocin induced Diabetic Rats and Their Role in Treatment-converted

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Vol.2 No.1 -8 : Effect of metformin on histopathological and immunohistochemical changes induced by high fructose intake in liver and brain of rats.

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By : Eman Ahmed Youssef

Abstract

Fructose is widely used as a food ingredient and has the potential to increase oxidative stress and related complications. The present study was designed to evaluate the role of metformin on histopathological and immunohistochemical changes in liver and brain of rat induced by high fructose intake. Forty male albino rats were divided into 4 groups. Groups I and II served as controls. Group III received 10 % drinking fructose solution for eight weeks. Group IV was received 10 % drinking fructose solution for eight weeks and treated with metformin (320 mg/kg/day) during the last 4 weeks of the experimental period. Rats were sacrificed after 8 weeks; liver and brain were excised and fixed in 10% neutral buffered formalin. Histopathological results: Fructose drinking manifested changes in liver and brain cerebral cortex. Liver changes were manifested as inflammation, apoptosis, dilated sinusoids, fibrosis, macrosteatosis, ballooned hepatocytes and marked collagen deposition, while brain changes were degenerating neurons, steatosis, karyorrhexis, and pyknotic nuclei in nuropil and lightly stained Nissl substance with cresyl violet. Metformin treatment eliminated histopathological changes in addition to decreased collagen deposition in liver and improved Nissl substance staining in brain. Immunohistochemical results showed increased immunostaining positivity of caspase-3 and inducible nitric oxide synthase ( iNOS) in liver and brain in fructose group . Reduced immunoreactivity of caspase-3 and iNOS in fructose plus metformin group either in liver or brain sections. In conclusion, the results suggest that the hepatoprotective and neuroprotective role of metformin on histopathological and and immunohistochemical changes induced by fructose could be attributed to its ability to reduce oxidative stress.

8. Effect of metformin on histopathological and immunohistochemical changes induced by high fructose intake in liver and brain of rats.

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