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Vol.7 No.1 – 4: Lactate dehydrogenase level as a COVID-19 biomarker

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By: Ahmed Abdelhalim Yameny

Society of Pathological Biochemistry and Hematology, Egypt

Email: dr.ahmedyameny@yahoo.com

DOI: 10.21608/jbaar.2021.173662

Abstract:

Background: Lactate dehydrogenase (LDH) is an intracellular enzyme found in cells in almost all organ systems, which catalyzes the interconversion of pyruvate and lactate, LDH has been proved to be a prognostic factor with

high accuracy in diseases involving multiple organ injuries such as acute heart failure (AHF) and severe acute pancreatitis (AP), LDH is a potential prognostic biomarker in patients with COVID-19. Patients and methods: This study included 217 non hospitalized patients with confirmed COVID-19 infection in Alexandria, Egypt, these study subjects were randomly selected irrespective of the age group and both genders were included, Assay procedure as manufactory instructions and Reagents of Chema Diagnostics Italy, LDH FL of reagent based on  DGKC recommendations. Results: The percentage of COVID-19 non hospitalized Patients relation to the serum LDH level, The present study included patients aged from14 years to 75 years mean age was 44.5 ±30.5 who were confirmed to have Covid-19 based on real-time reverse transcription-polymerase chain reaction, female gender was more frequent (n=119, 54.8%) than Male gender (n=98, 45.2%). This study reveals high serum LDH levels in 147 patients (67.7%), which was a significant biomarker for COVID-19 diagnosis in non-hospitalized patients (Outpatients and patients under home observation), with a p-value is 0.024 which less than 0.05. Conclusion and Recommendations: Serum LDH level is a good biomarker of infection in COVID-19 Outpatients and patients under home observation increased by 67.7%, many other studies revealed LDH is a good marker for COVD-19 hospitalized patients and severe infection.

Lactate dehydrogenase level as a COVID-19 biomarker-converted (3

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Vol.1 No.6 -8 : Acrylamide induced histochemical and immunohistochemical alterations in rat kidney cortex

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By : Safia Mohamed Hassan1 and Eman Ahmed Youssef 2

Abstract

Acrylamide represents an industrial chemical and has become one of the main public health concerns since it was detected in extensively consumed food items. The present study was planned to investigate the effects of two doses of acrylamide on some enzyme activities and immunoreactivity of some immunohistochemical parameters in kidney cortex of male rats. Rats were randomly divided equally into three groups. Group (I) was control, group (II) was given acrylamide for 6 months orally in a dose of 0.05 mg/ /Kg dissolved in water for 3 doses per week and group (III) was administered acrylamide in a dose of 0.5 mg by the same way as in group II. At the end of the experiment all animals were sacrificed under anesthesia, kidney was immediately removed and processed for histochemical and immunohistochemical studies. The results revealed that the activities of lactic dehydrogenase (LDH) and xanthine oxidoreductase (XOR) were significantly increased, while succinic dehydrogenase (SDH) activity was decreased significantly compared to control group. On the other hand, immunohistochemical results showed that acrylamide significantly reduced the immunoreactions of endothelial nitric oxide synthase (e- NOS) in addition to significant increase in immunoreactivity of inducible nitric oxide synthase (i- NOS) and insignificant increase in alpha smooth muscle actin (α-SMA) with respect to control group. Over all, these results suggested that the deleterious effect in kidney tissue resulted from oral administration of acrylamide, most probably due to oxidative stress and lipid peroxidation.

8. Acrylamide induced histochemical and immunohistochemical alterations in rat kidney cortex
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