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Vol.2 No.5 -7 : Folic acid ameliorates L-thyroxin induced hepatotoxicity and oxidative stress in albino rats.

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By : Somya Y. Shalaby¹, Saber A. Sakr¹, Ehab Tousson², Mohamed Rabea¹

Abstract

Thyroid hormones have been known to regulate the energy metabolism of most tissues including liver. Alterations in their normal levels cause some biochemical and clinical abnormalities such as hypothyroidism and hyperthyroidism. The present study evaluated the effect of thyroid hormone, L-thyroxin on liver of albino rats. Additionally the ameliorating role of folic acid supplementation was investigated. Fifty male albino rats were randomly divided into five groups (group I, control; group II, folic acid; group III, L-thyroxin sodium administration (100 μg/kg / body weight); group IV, L-thyroxin and folic acid group and V, recovery group). The results showed that there were a significant increase in ALT, AST, MDA and nitric oxide in L-thyroxin treated rats as compared to control group. On the other hand, a significant decrease in glutathione (GSH) in L-thyroxin treated rats as compared to control group. Histological results showed that liver sections of L-thyroxin group showed histopathological lesions such as leucocytic infiltrations, congestion of central and portal veins and cytoplasmic vacuolation of hepatocytes with the presence of pyknotic nuclei, in addition to fatty infiltration. Immunohistochemical results revealed that strong positive expression of PCNA, P53, and Bcl-2 were detected in the liver section in L-thyroxin treated rats and recovered rats as compared to control and folic acid groups. However; mild to moderate positive expressions of PCNA, P53, and Bcl-2 were observed in rats treated with L-thyroxin and folic acid in liver section. This reflects oxidative stress associated with hyperthyroid state.

7. Folic acid ameliorates L-thyroxin induced hepatotoxicity and oxidative stress in albino rats.
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Vol.1 No.5 -5 : Apoptotic Marker Alternations in the Spleen of Experimentally Hyperthyroid and Hypothyroid Rat.

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By : Ezar Hafez; Ahmed Masoud; Magdy Barnous; Ehab Tousson

Abstract

Apoptosis plays a critical role in the development and homeostasis of multicellular organisms, especially those with high cell turnover such as the lymphoid system. The current study aimed to examined the effects of changes in thyroid hormones on apoptosis of spleen in male rats. 30 rats were equally divided into three groups (10 animals each). G1, control group in which animals did not received any treatment; G2, Hypothyroid group in which rats received 0.05% 6-n-propyl-2-thiouracil (PTU) in drinking water for 6 weeks; G3, Hyperthyroid group in which rats received 100 μg/Kg L-Thyroxin sodium administration in drinking water for 6 weeks. In the present study; serum T3 and T4 concentrations were depressed and serum TSH concentration was significantly elevated in rats receiving PTU-induced hypothyroidism. On the other hand; serum T3 and T4 concentrations were significantly elevated and serum TSH concentration was depressed in rats receiving L-Thyroxin sodium-induced hyperthyroidism. In the current study; spleen in both hypothyroid and hyperthyroid rats revealed many of abnormalities as marked disruption of spleen structure, loss in distinction between the white and red pulps, degeneration and vacuolation with an increased in the lymphocyte population. Also, a significant increase in p53 and Caspase3 apoptotic cells and a significant decrease in Bcl-2 antiapoptotic cells in the spleen tissues revealed the possibility of the apoptosis occurrence after PTU or Thyroxin administration in the case of hypothyroidism and hyperthyroidism.

5. Apoptotic Marker Alternations in the Spleen of Experimentally Hyperthyroid and Hypothyroid Rat.
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