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Vol.2 No.3 -5 : The protective effect of Coriandrum sativum L. oil against liver toxicity induced by Ibuprofen in rats.

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By : Hoda H. Baghdadi1,Fatma M.El-Demerdash2,Eman H. Radwan3, Sodfa Hussein4

Abstract

Ibuprofen (IBU) is a Non-steroidal anti-inflammatory drugused in the treatment of pain, fever and in inflammation.Coriandrum sativum is cultivated for its aromatic and medicinal uses. The present study aims to evaluatethe protective effect of Coriandrum sativum volatile oil on hepatotoxicityof IBU in rats. Five groups of albino rats were used.Group l(control),groupll (C.sativum oil,40 mg/kg B.W. for 14 day), group lll (IBU group,100mg/kg bodyweight B.W., for 14 day), group lV (IBU+ C.sativum oil) and groupV (recovery group).The activity of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured in the liver of different groups addition to the histological examination of the sections of liver. The results showed that IBU caused a significant decrease in the activity of ALT and AST in the liver. The histological examination of the liver showed many pathological changes. Administration of coriander volatile oilin the combination with IBU was able to significantly increase the activity of both AST and ALT, in the liver and caused a significant decrease in the deleterious effect induced by IBU. The present results confirm that the antioxidant activity of volatile oil of the Coriandrum sativum L., against hepatotoxicity of IBU.

5. The protective effect of Coriandrum sativum L. oil against liver toxicity induced by Ibuprofen in rats.
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Vol.2 No.1 -8 : Effect of metformin on histopathological and immunohistochemical changes induced by high fructose intake in liver and brain of rats.

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By : Eman Ahmed Youssef

Abstract

Fructose is widely used as a food ingredient and has the potential to increase oxidative stress and related complications. The present study was designed to evaluate the role of metformin on histopathological and immunohistochemical changes in liver and brain of rat induced by high fructose intake. Forty male albino rats were divided into 4 groups. Groups I and II served as controls. Group III received 10 % drinking fructose solution for eight weeks. Group IV was received 10 % drinking fructose solution for eight weeks and treated with metformin (320 mg/kg/day) during the last 4 weeks of the experimental period. Rats were sacrificed after 8 weeks; liver and brain were excised and fixed in 10% neutral buffered formalin. Histopathological results: Fructose drinking manifested changes in liver and brain cerebral cortex. Liver changes were manifested as inflammation, apoptosis, dilated sinusoids, fibrosis, macrosteatosis, ballooned hepatocytes and marked collagen deposition, while brain changes were degenerating neurons, steatosis, karyorrhexis, and pyknotic nuclei in nuropil and lightly stained Nissl substance with cresyl violet. Metformin treatment eliminated histopathological changes in addition to decreased collagen deposition in liver and improved Nissl substance staining in brain. Immunohistochemical results showed increased immunostaining positivity of caspase-3 and inducible nitric oxide synthase ( iNOS) in liver and brain in fructose group . Reduced immunoreactivity of caspase-3 and iNOS in fructose plus metformin group either in liver or brain sections. In conclusion, the results suggest that the hepatoprotective and neuroprotective role of metformin on histopathological and and immunohistochemical changes induced by fructose could be attributed to its ability to reduce oxidative stress.

8. Effect of metformin on histopathological and immunohistochemical changes induced by high fructose intake in liver and brain of rats.
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Vol.1 No.6 -11 : Histological and Biological Effects of Some Soft Drinks on Male Albino Rats

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By : Nehad R. EL-Tahan1 and Rania A. Ahmed2

Abstract

sodas that could have a caloric or noncaloric sweetener. Several studies showed that the excessive consumption of soft drinks leads to harmful health effects. This study aimed to determine the effects of some soft drinks on the public health by examining the histological structure of kidney, body weight, biochemical values, bone mineral density (BMD ) and bone Xray . Twenty male rats, (weighing 140 ±5 g) , were divided into 4 groups. ; Group 1 (the control group) fed only on basal diet. ; Group 2 received cola diet soft drink ; Group 3 received orange soft drink and Group 4 received lemon soda soft drink. For histopathological examination, The kidneys were removed. The bone mineral density of the rats was assessed using Dual-energy X-ray absorptiometry (DXA). The body weight change and blood values were also determined. The histological examination of the kidneys showed general glomerular congestion, vacuolation, intertubular bleeding, tubular necrosis and hypotrophy of glomeruli . The weight gain was higher in the groups consuming diet drinks than the control group and the other group rats. There was a significant decrease in the bone mineral density of test groups when compared to the control group .

11. Histological and Biological Effects of Some Soft Drinks on Male Albino Rats
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Vol.1 No.4 -1 : Histopathological effects of Diflubenzuron and Chromafenozide (IGRs) on egg development in the cotton leafworm Spodoptera littoralis (Boisd) (Lepidoptera, Noctuidae).

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By : Ahmed, K. S.; El-Bermawy, S. M.; Ismael, E. H.; Abdel-Gawwad R. M. and Saleh, T. A.*

Abstract

The present study was undertaken to investigate the possible pathogenic impacts of the LC50 of the two insect growth regulators: the chitin synthesis inhibitor, Dimilin® (Diflubenzuron), and the ecdysone agonist, Virtu® (Chromafenozide) on the ovarian tissue of adult female Spodoptera littoralis. Therefore, the LC50 (1.3 & 3 ppm of Dimilin® and 0.1 ppm of Virtu®) were applied to the 2nd and 4th larval instars, respectively. The meroistic polytrophic ovariole of S. littoralis is divided into a terminal filament, a germarium and a vitellarium; the germarium contains the oogonia and the follicular cells that surround the oocytes to form the ovarian follicles in the vitellarium. The ovarian follicles of treated F1 females with Dimilin® exhibited vacuolization, stuffing of follicular sheath, loss of vitellin and degeneration of many cell components of follicular epithelium and oocytes. These obvious signs of damage were increased when using 4th larval instars treatment than the 2nd.Also, damage was pronounced in the ovarioles of the Virtu® treated F1 females. Ovariole growth was stunted and vitellogenesis and chorion formation were inhibited. So, this work shows the high potency and efficacy of the two IGRs on developed ova of S. littoralis

1. Histopathological effects of Diflubenzuron and Chromafenozide (IGRs) on egg development in the cotton leafworm Spodoptera littoralis (Boisd) (Lepidoptera, Noctuidae).
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