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Vol.10 No.1 – 6: Histopathology and Histochemistry study of surgically removed appendix of some patients in Misan Governorate, Iraq

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Muhanad Mahdi Mohammed1, Hawrra Jabbar Mohammed 2, Eman Kamil Aati 3

1 College of Dentistry, University of Misan, Amarah, Maysan, Iraq.

2 Department of Biology, College of Sciences, University of Misan, Amarah, Maysan, Iraq.

3 College of Nursing, University of Misan, Amarah, Maysan, Iraq.

ABSTRACT

This study sheds light on the histopathology structure and histochemistry of the appendix. For a further understanding of the supplement’s function. Where (neutrophils) were observed in all the specimens as signs of inflammation. The inflammation extended from the epithelial layer to the muscularis layer.

 Furthermore, it showed cells (neutrophils) Ulcerated in the mucosa, Fat necrosis and Atrophy in payer patches in the submucosa layer, and Hypertrophy in smooth muscle with damage for the same layer, The study included 40 samples, they are suffering from appendicitis, whose weights ranged between 45-75 kg, respectively. The study period was from (1/4/2023 to 7/4/2023). Eosin and hematoxylin, a tissue examination approach, and periodic acid Schiff stains, a histochemistry method, are used in this work, as well as show that PAs staining gave a strong reaction in all layers of the appendix, evidence for the presence of neutral carbohydrates such as neutral mucin and glycogen.

Histopathology and Histochemistry study of surgically removed appendix of some patients in Misan Governorate

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Vol.2 No.11 -2 : Efficacy of saffron extract and fenugreek seeds supplementation on liver of streptozotocin induced diabetic rats

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By : Doaa A. Ali , Hanaa Serag , Ahmed Abdeen and Radwa Refaat

Abstract

The present study aimed to investigate the possible hypoglycemic and hepatoprotective effects of saffron extract (100mg/kg b.wt) and fenugreek supplementation (1.4 g/day/kg b.wt) in STZ- induced diabetic rats at biochemical, histological, histochemical and ultrastructural levels. The results indicated that a single dose of STZ (50 mg /kg b wt, i.p) caused a significant increase in serum and liver total lipids, total cholesterol and triglyceride as well as serum low density and a very low density lipoprotein, accompanied by an increase in serum glucose level and oxidative stress marker, malondialdehyde and hydrogen peroxide level as well as serum aspartate and alanine aminotransferase activities. The results also reported a significant decrease in the high density lipoprotein, serum and liver total protein, serum albumin and globulin contents and A/G ratio, as well as a significant decrease in serum insulin level and the liver enzyme activities and the hepatic glutathione content. Histologically, STZ treated group showed degenerative changes in the pancreas and alteration in the structural integrity of the hepatocytes and their intracellular organelles. Histochemically, liver sections of STZ treated animals displayed marked decrease in the glycogen and total protein contents. On the other hand, the intake of saffron or fenugreek minimize the disturbance observed in the most tested parameters resulted from STZ administration and improve the liver structure and functions. Finally, it can be concluded that the intake of natural product such as saffron or fenugreek may be effective in reducing the diabetic liver injury induced by streptozotocin and the fenugreek supplementation showed more amelioration than saffron extract.

2-Efficacy-of-saffron-extract-and-fenugreek-seeds-supplementation-on-liver-of-streptozotocin-induced-diabetic-rats
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