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Vol.8 No.4 – 2:Non-invasive follow-up of Egyptian patients infected with Helicobacter pylori by quantification of H. pylori circulating antigen in serum using ELISA

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By: Hager R. Fawzy1, Asmaa M. Abdelmageed2, Mahmoud A. Shoulkamy1, Mohamed Abdel Wahab2, Hisham Ismail3, *

1Microbiology Division, Botany & Microbiology Dept., Faculty of Science, Minia University, Minia 61519, Egypt

2Gastrointestinal Surgery Center, Faculty of Medicine, Mansoura University, Mansoura 35516, Egypt

3Biochemistry Division, Chemistry Dept., Faculty of Science, Minia University, Minia 61519, Egypt.

Abstract

Clinicians still wish to determine if H. pylori-infected patients have been cured after specific treatment. The present study aimed to evaluate the reliability of the H. pylori circulating antigen (HpCAg) test for noninvasive screening of H. pylori infection and assessment of cure after specific treatment. Sera of 134 symptomatic individuals (81 males & 53 females, aged 23-68 yr) were screened for HpCAg using ELISA. H. pylori infection was confirmed using a gold standard based on culture, rapid urease test, and histology testing. The detection rate of HpCAg was 69% among screened individuals. The gold standard confirmed H. pylori infection in 93% of individuals showing HpCAg in their sera. In addition, 31% of infected patients were excluded for their drug resistance. Eligible individuals received a standard triple therapy regimen including Lansoprazole, Clarithromycin, and Amoxicillin twice daily for 14 days. Six weeks later, the HpCAg testing was repeated to evaluate the treatment outcome. HpCAg was not detected in 78 % of treated individuals. Furthermore, the levels of HpCAg were significantly decreased (p < 0.001) in the sera of non-responders. In conclusion, the detection of HpCAg is a reliable non-invasive approach for screening and follow-up of H. pylori-infected individuals after treatment, especially in developing countries.

Non-invasive-follow-up-of-Egyptian-patients-infected-with-Helicobacter-pylori-by-quantification-of-H.-pylori-circulating-antigen-in-serum-using-ELISA

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Vol.5 No.4 – 11: Molecular screening of Helicobacter pylori among Egyptian patients with decompensated liver cirrhosis

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By: Mohamed Y. Nasr1, Ehsan Hassan2, Ghada Hassan1, Asmaa Ibrahim3, Salwa. E. Mohamed1, Amal Abdel-Aziz1

1. Genetic Engineering and Biotechnology Research Institute, University of Sadat City (GEBRI, USC), Egypt

2. National Hepatology and Tropical Medicine Research Institute, Cairo, Egypt

3.Diagnostic and Research Unit of Parasitic Diseases (DRUP), Department of Medical Parasitology, Kasr Al-Ainy Faculty of Medicine, Cairo University, Cairo, Egypt

Abstract

Background: Globally Helicobacter Pylori (H. Pylori) infection and liver diseases are renowned with their high incidence rate. Recent studies have observed the relation between H. Pylori infection and liver disease progression to cirrhosis. This study aimed to determine the prevalence of H. pylori in liver cirrhotic patients. Patient and Methods: This study was conducted on 100 liver cirrhotic patients to detect H. Pylori infection based on UreA gene detection in ascites using nested polymerase chain reaction (nPCR). Biochemical analysis was performed on ascitic fluid, serum, complete blood count (CBC), and polymorph nuclear leukocytes count (PMN). Result: H. Pylori infection was detected in 75% of patients with liver cirrhosis. In ascitic fluid, H. pylori infection showed a highly significant association with albumin and PMN (P=0.0001) and also with other biochemical analyses including serum ascites albumin gradient (SAAG), specific gravity, and glucose (P=0.005, 0.0031, and 0.02, respectively). In serum only, the infection showed significant association with alkaline phosphatase, albumin, and hemoglobin with P values of 0.008, 0.014, and 0.008, respectively. Conclusion: The current study results shed light on the significant role of H. Pylori infection in ascites of patients with decompensated end-stage liver cirrhosis suggesting a prospective relation between H. pylori infection and liver diseases progression due to hepatic infections especially HCV.

Molecular screening of Helicobacter pylori among Egyptian patients with decompensated Liver cirrhosis 1

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