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Vol.10 No.1 – 9: Assessing Gene Expression of factor XIII-A in Iraqi patients with FXIII deficiency

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Murtadha A. Al- Shami¹, Asmaa Mohammed Saud¹

1Biotechnology Department¹‚ College of Science‚ University of Baghdad ‚ Baghdad, Iraq

ABSTRACT

Coagulation factor XIII (FXIII) circulates in plasma as a pro-transglutaminase heterotetrameric complex (FXIIIA2B2), which upon activation by thrombin and calcium covalently crosslinks preformed fibrin polymers. The heterotetrameric complex is composed of a catalytic FXIIIA2 subunit and a protective/regulatory FXIII-B2 subunit coded by F13A1 and F13B genes, respectively. The study aimed to assess the level of expression of the FXIII-A gene in seven Iraqi families affected with FXIII deficiency. Samples of whole blood were collected from seven Iraqi families that were characterized with F13 deficiency register in the Hemophilia Ward, Children Welfare Teaching Hospital, Medical City, Baghdad. These samples were divided into three groups according to the genotype, which included affected homozygous recessive and heterozygous with control healthy group. RNA was isolated from whole blood samples and gene expression levels of the F13 A1 gene was determined using quantitative Real-Time polymerase chain reaction (RT-qPCR), the result revealed that the expression levels of the F13A gene in heterozygous parents were significantly increased compared with the homozygous affected and control group. The (ROC) curve was analyzed in this study to determine the optimal cut-off value for F13 expression and showed a very good predictor for FXIII deficiency diagnosis with “Area under the ROC Curve.” AUC equals 0.844 and p< 0.001.

Assessing Gene Expression of factor XIII-A in Iraqi patients with FXIII deficiency

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Vol.5 No.3 – 11 : Study the effect of pheromones on gene expression in the brain of the honeybee Apis mellifera

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By: Husham Naji Hameed

Department of Biology, College of Education, University of Samarra, Tikrit, Iraq

Abstract:

          This Research deals with the effect of pheromones  queen mandibular pheromone onegene expression of brain adult for workers honeybee Apis mellifera, these expressions can explain the response behavior induced by pheromones  QMP, the impact of gene expression by affecting the movement of workers A. mellifera in the colony of honeybees in the case of the presence of the Queen. Queen’s absence means the lack of workers as a result of the lack of pheromones  (QMP) or the presence of a few compared to the presence of the Queen.  The behavior of workers is linked to the existence of pheromones released by the Queen .

Study the effect of pheromones on gene expression in the brain of the honeybee Apis mellifera-converted

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Vol.5 No.2 – 5 : Molecular expression and single nucleotide polymorphisms of IL17A gene among etanercept-treated rheumatoid arthritis patients

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By: Aseel S. Mahmood1, Abdul-Kareem A. Al-kazaz1, Khadier Z. Mayouf2 and Ali H. Ad’hiah3

1Biotechnology Department, College of Science, University of Baghdad, Baghdad, Iraq.

2College of Medicine, University of Baghdad, Baghdad, Iraq.

3Tropical-Biological Research Unit, College of Science, University of Baghdad, Baghdad, Iraq.

Abstract

Molecular expression (reverse transcription-quantitative polymerase chain reaction; RT-qPCR) and DNA-sequencing-based single nucleotide polymorphisms (SNPs) of interleukin 17A (IL17A) gene were determined in 51 etanercept-treated Iraqi rheumatoid arthritis (RA) patients and 45 control. The results revealed that the relative expression (2-∆∆Ct) of IL17A gene was increased by 1.28 ± 0.29 fold in RA patients, and such profile was approximated in male (1.66 ± 0.58) and female (1.01 ± 0.28) patients.  With respect to PCR-amplified DNA sequences, out of the 10 encountered SNPs, two SNPs (rs8193038 and rs3819025) showed allele frequencies that exceeded 10%. The rs8193038 SNP allele and genotype frequencies showed no significant variations between RA patients and control. The second SNP (rs3819025) was observed to have three genotypes (AA, AG and GG). Among these genotypes, it was observed that the homozygous genotype of mutant allele (GG) was only recorded in patients with a frequency of 13.7%, while none of the control had this genotype. Such difference was significant even after the correction of probability (pc = 0.05), and the associated OR was 15.34 (95% C.I.: 1.39 – 169.24). It was also observed that G allele showed a significant increased frequency in patients (25.5 vs. 12.2%; OR = 2.46; 95% C.I.: 1.14 – 5.30; p = 0.015), while A allele frequency was significantly decreased (74.5 vs. 87.8%; OR = 0.41; 95% C.I.: 0.19 – 0.88; p = 0.015). However, the significance in both cases was lost when the probability was corrected.  It was also observed that there was no significant impact of the rs3819025 SNP genotypes on expression of IL17A gene. In conclusion, IL17A gene showed an increased expression in RA patients, and rs3024419 SNP is suggested to be associated with an increased risk to develop the disease in Iraqi population.

Molecular-expression-and-single-nucleotide-polymorphisms-of-IL17A-gene-among-etan

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