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Vol.10 No.1 – 3: Exploring the Phytochemical Composition of Methanolic Extract from Launaea nudicaulis: Investigating its Antioxidant, Anticancer, and Anti-Dengue Activities against Aedes aegypti

Abadi M. Mashlawi1, Areshi S.1, Naimah Asid H Alanazi2, Gamal Mohamed 3, Abdel Daim Z. J.1, Mohammed A. Alfattah1, Dalia A. Mohamed4, Eman Fayad5 and Salama A. Salama1 *

1. Biology Department, College of Science, Jazan University, Jazan City/ Postal code 82817/ Saudi Arabia

2. Department of Biology, College of Sciences, University of Ha’il, KSA.

3. Human Anatomy Department, Faculty of Medicine, Jazan University, KSA.

4- Biology Department, University College of Al Khurmah, Taif University P.O. Box 11099, Taif 21944, KSA

5- Biotechnology Department College of Sciences, Taif University P.O. Box 11099, Taif 21944 KSA

ABSTRACT

Asteraceae is considered to be one of the most medicinal significant families. One family species Launaea nudicaulis is globally used in traditional medicine to treat various ailments. The objective of the current investigation was to evaluate the chemical composition of the methanol extract of L. nudicaulis and its possible biological impacts. In the present study, the GC-MS analysis identified 10 components, with fatty acid derivatives, terpenes, and alkaloids as the main compound classes. The major components are (2E,6E,10E)-3,7,11,15-Tetramethyl-2,6,10,14-hexadecatetraenyl acetate (29.20%), 2-Ethylhexanoic acid (24.57%) and thunbergol (20.53%). The antioxidant assay shows the L. nudicaulis extract has good DPPH radical scavenging ability with an IC50 value for the shoot extract is 32.52 mg/L., which is linked to its phytochemical composition. The cytotoxicity results demonstrate selective anticancer effects of the extract against HepG- 2 and PC3 cells compared to normal cells. The MeOH extract demonstrated an IC50 value of 38.56 and 46.30 µg/ml of HePG-2 and PC3 cancer cells, respectively. Additionally, the extract’s excellent position as a perfect natural insecticide is highlighted in the current work. Using methanol as a solvent, the extract shows great larvicidal potential against Aedes aegypti L. Researchers are encouraged to employ natural insecticides when the insects are still young, not when they are older. The findings validate the traditional therapeutic applications of wild plants.Asteraceae is considered to be one of the most medicinal significant families. One family species Launaea nudicaulis is globally used in traditional medicine to treat various ailments. The objective of the current investigation was to evaluate the chemical composition of the methanol extract of L. nudicaulis and its possible biological impacts. In the present study, the GC-MS analysis identified 10 components, with fatty acid derivatives, terpenes, and alkaloids as the main compound classes. The major components are (2E,6E,10E)-3,7,11,15-Tetramethyl-2,6,10,14-hexadecatetraenyl acetate (29.20%), 2-Ethylhexanoic acid (24.57%) and thunbergol (20.53%). The antioxidant assay shows the L. nudicaulis extract has good DPPH radical scavenging ability with an IC50 value for the shoot extract is 32.52 mg/L., which is linked to its phytochemical composition. The cytotoxicity results demonstrate selective anticancer effects of the extract against HepG- 2 and PC3 cells compared to normal cells. The MeOH extract demonstrated an IC50 value of 38.56 and 46.30 µg/ml of HePG-2 and PC3 cancer cells, respectively. Additionally, the extract’s excellent position as a perfect natural insecticide is highlighted in the current work. Using methanol as a solvent, the extract shows great larvicidal potential against Aedes aegypti L. Researchers are encouraged to employ natural insecticides when the insects are still young, not when they are older. The findings validate the traditional therapeutic applications of wild plants.

Exploring the Phytochemical Composition of Methanolic Extract from Launaea nudicaulis Investigating its Antioxidant, Anticancer, and Anti-Dengue Activities against Aedes aegypti

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Vol.8 No.4 – 3:Antitumor efficacy of Urtica sp. leaves extract: in vitro and in vivo studies

By: Rania Shams El Deen Fakher El Deen1, Sabry Ali El-Naggar2, Eman El-Nahass2,Karim Samy El-Said3

1 Nutrition and Food Science, Faculty of Specific Education, Tanta University, Egypt

2 Zoology Department, Faculty of Science, Tanta University, Egypt

3 Biochemistry Division, Chemistry Department, Faculty of Science, Tanta University, Egypt

Abstract

Background: Natural products demonstrated potential for use in medicine and pharmaceuticals. Objective: This study evaluated the phytochemical composition of Urtica sp. leaves extract (USLE), and its in vitro and in vivo antitumor effects. Methods: HepG-2 and MCF-7 cell lines were used to evaluate the IC50, apoptosis, and cell cycle analysis of USLE. Forty female CD-1 mice were equally divided as follows; Gp1 was control; Gp2, Gp3, and Gp4 were inoculated with 1 × 106 of Ehrlich ascitic carcinoma (EAC)-cells/mouse. Then, Gp3 had injected with Cisplatin (2 mg/kg) intraperitoneally (i.p). Gp4 had injected with USLE (100 mg/kg) i.p. as in Gp3. Total tumor volume, total tumor cell count, and live and dead EAC-cells were determined, also biochemical and histopathological investigations were evaluated. Results: Data showed that the USLE had cytotoxic and antitumor effects against the HepG-2, MCF-7, and EAC-bearing mice. Biochemical and histopathological investigations showed an improvement in the liver and kidney tissues upon treatment of EAC-bearing mice with USLE. Conclusion: The results showed that the USLE can stop tumor growth and cause tumor cells to die.

Antitumor-efficacy-of-Urtica-sp.-leaves-extract-in-vitro-and-in-vivo-studies

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