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Vol.10 No.2 – 5: Modulating effect of milk thistle (Silybum marianum) oil on CD34 and vimentin expressions in fibrotic and cirrhotic liver tissues induced by CCl4 in mice

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Nabila I. El-Desouki1,*, Mohamed A. Basyouny1, Soha G. Okba1 , Rabab A. Hegazy2,*, Buthina S. Alshammari1

1Department of Zoology, Faculty of Science, Tanta University, Tanta 31527, Egypt

2 Department of Biology, University College in Darb, Jazan University, Al-Darb, Jazan 45142, Saudi Arabia

Abstract:

Aim: evaluate the impact of milk thistle (Silybum marianum) on CD34 and vimentin expression in hepatic fibrosis and cirrhosis induced with CCl4 in experimental mice. Methods: GI: normal group given no therapy; control group. GII: received a daily dose (1mL/kg/bw/d) of milk thistle oil M.T.O for 4 weeks; GIII&GIV: injected i.p. with (1:1 ratio) mixture of CCl4 and olive oil (1mL/kg/bw) twice weekly for 4 weeks to induce fibrosis, and for 6 weeks to induce cirrhosis. Gp5 and Gp6: fibrotic and cirrhotic groups administered M.T.O as in Gp2. The results showed that liver sections of Gp1 and Gp2 showed normal moderate to strong CD34 expression in the endothelial cells of the blood sinusoids and many hepatocytes. The liver tissues of Gp3 and Gp4 expressed decrement CD34 immunoreactivity in many hepatic lobules. The liver sections of Gp5 or Gp6 showed restoration of CD34 expression in most of the hepatic tissues. In Gp1 and Gp2, the vimentin was expressed as weak or moderate immunostaining in the endothelial cells and connective tissues (wall of the blood sinusoids, central portal veins, and portal tract stroma). The liver sections of Gp3 and Gp4 showed overexpression of vimentin immunoreactivity. The treatment with M.T.O in Gp5 and Gp6 showed improvement and recovery of vimentin expression in the hepatic lobules. Conclusion: M.T.O. treatment improved the hepatic injury induced in fibrotic or cirrhotic tissues by CCl4 injection and could be recommended for patients with fibrotic and cirrhotic liver diseases.

Modulating effect of milk thistle (Silybum marianum) oil on CD34 and vimentin expressions in fibrotic and cirrhotic liver tissues induced by CCl4 in mice

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Vol.2 No.5 -6 : Serum hyaluronic acid as non invasive biomarker to predict liver fibrosis in viral hepatitis patients.

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By : Ayman E. El Agroudy1, Mohamed S. Elghareb2, Emad H. Elshahat3, Ezar H. Hafez4, Tamer A. Addissouky5

Abstract

Fibrosis is a hallmark histologic event of viral hepatitis and is characterized by the excessive accumulation and reorganization of the extracellular matrix (ECM). The gold standard for assessment of fibrosis is liver biopsy. As this procedure has various limitations, including risk of patient injury and sampling error. Serum Hyaluronic acid as non invasive marker for liver fibrosis is desirable. The present study aims to determine the serum hyaluronic acid (HA) levels as biochemical marker of hepatic fibrosis and cirrhosis and correlate it with the degree of hepatic fibrosis. Serum HA level in chronic hepatitis patients (n=60) are divided into two groups, group1: included 30 patients positive for anti-HCV (antibodies), group 2 included 30 patients positive for HBsAg, and controls (n=10) were assessed by ELISA and liver histopathological parameters were evaluated by the modified Knodell score and microscopic examination of liver biopsies from patients. Individuals in healthy control group have normal levels of HA (mean 14.3, SD: 5.5) while the levels of HA were elevated in patients of HCV alone (mean 103.6±28.0) and in patient of HCV (mean 104.5± 37.5).Also levels of HA were poorly elevated in HBV alone (mean 62.2± 15.5) and in HBV (mean 45.8± 12.4). showed that serum HA levels are well correlated with HAI in patients of HBV & HCV groups where, there was significant increase in HA levels by increase of HAI by liver biopsy P < 0.001.HA levels and stages of fibrosis were well correlated in patients of HBV and HCV group. Where, this is a significant increase in HA levels when Considering F0 to F6 scores by liver biopsy (P < 0.001). Serum HA is a useful non-invasive marker of liver fibrosis. There is a strong positive correlation between serum HA levels and degree of liver fibrosis. The concentration of serum HA rises according to progression of liver fibrosis.

6. Serum hyaluronic acid as non invasive biomarker to predict liver fibrosis in viral hepatitis patients.

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