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Vol.9 No.3-5:Antifungal activity of essential oil of Syzygium aromaticum on Rhynchosporium secalis, the causal agent of barley Scald

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By: Naima Essouaadi1,2, Aicha El aissami3, , Abduladeem G.M.Al-Selwi4, Sanae Karim3, Mustapha Labhilili2, Houda Khalifi 1, Soukaina Hamoumi 1, Mohamed Benchacho1 andFatiha Bentata2*

1 Botanical laboratory, biotechnology and protection of plants, university of Science Kénitra Morocco

2Aromatic and Medicinal Plants Research Unit, Institute of Agricultural Research Rabat Morocco

3 Botanical laboratory, biotechnology and protection of plants, University of Science of Rabat –       Morocco

4Faculty of Medicine and Pharmacy, Rabat-Morocco

Abstract

Scald caused by Rhynchosporium secalis is one of the most devastating barley foliar diseases worldwide. Morocco has not been spared from this scourge. Our research focused on the antifungal effects of clove essential oil. Essential oils are extracted from the flower buds of aromatic plants using hydrodistillation. According to this study, the obtained extraction rate (9.07%) is quite satisfactory, making the plant a valuable natural resource. The aroma essential oil was identified by gas chromatography, and its main component was eugenol with a content of more than 52%, followed by eugenol acetate (25.94%), caryophyllene (7.845%) and caryophyllene oxide (1.74%). Two R. secalis isolates were tested for antibacterial efficacy. For the two isolates (Rs1 and Rs2) at a low concentration of 0.4 µl/ml, complete inhibition was observed under the action of S. aromaticum essential oil. According to our results, the essential oil has an antifungal effect on Scald.

Antifungal-activity-of-essential-oil-of-Syzygium-aromaticum-on-Rhynchosporium-secalis-the-causal-agent-of-barley-Scald

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Vol.5 No.2 – 8 : The curative effect of Cymbopogon citrates volatile oil against chlorambucil drug toxicity

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By: ZakariaTeleb1, KamiliaTaha2, Sobhy Hassab El-Nabi3, Islam El-Garawani3, Gouda T. Dawoud2, Samraa S. El-Shafey3 and Hanaa M. El-Esawy3

1 Department of Biochemistry, National Organization for Drug Control and Research, MHP, Egypt.

2 Department of Phytochemistry, National Organization for Drug Control and Research, MHP, Egypt

3 Department of Zoology, Faculty of Science, Menoufia University, Egypt.

Abstract

Chlorambucil (CLB) is a bifunctional alkylating drug widely used as an anticancer agent and immunosuppressant. CLB mutagenicity, teratogenicity and carcinogenicity are indicated based on their structure and clinical history. This study aims to evaluate the antigenotoxic effect of Cymbopogon citratus essential oil, CC, (75 mg/kg) against CLB (7.5 mg/kg) genotoxicity in rats. GC/MS for essential oil has identified 19 compounds representing approximately 99.7% Geranial was the most abundant (53.5%) followed by Neral (35%) and Myrcene (5.3%).  The lowest was α-Muurolene (0. 1%). The marked damage was observed in total genomic DNA and total protein profile of CLB-intoxicated rat’s spleen tissues. Lymphocytes single strand breaks of treated rats were examined by comet assay after CC had ameliorated these effects in a time dependent manner (5, 10 and 15 days) for spleen and after 48 hours for lymphocytes. In conclusion, this study suggests that Cymbopogon citrates oil possesses antigenotoxic potential in CLB-intoxicated rats. It can constitute natural, new and safe co-therapeutics.

The curative effect of Cymbopogon citrates volatile oil against chlorambucil drug toxicity-converted

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