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Vol.8 No.1 – 3: 8-hydroxy-2′-deoxyguanosine and TP53 in Egyptian Patients with Hepatitis C Viral Chronic Liver Diseases: Insight into the Pathogenesis and Predictive Force

By : Hoda M. El-Emshaty1,*, Somaia M. Osman 2, Fathy M. El-Taweel2, Mohamed M. El-Hemaly1, Hisham Ismail 3

1Gastrointestinal Surgery Center, Faculty of Medicine, Mansoura University, Mansoura, Egypt

2Chemistry Dept., Faculty of Science, Damietta University, New Damietta, Egypt.

3Biochemistry Division, Chemistry Dept., Faculty of Science, Minia University, Minia, Egypt.

Abstract

Reactive oxygen species (ROS) is excessively generated during tumor development yielding the oxidatively modified products of proteins and DNA. These DNA alterations could contribute to the development of cancer through the activation of oncogenes and inactivating tumor suppressor genes (TSGs). Therefore, 8-OHdG DNA oxidative damage and TP53 protein expression were evaluated amongst HCV-Chronic liver disease patients to explore their possible role in hepatocarcinogenesis and to predict HCC development at early stages. A total of 141 patients with HCV-related liver diseases; 69 with hepatocellular carcinoma and 72 with liver cirrhosis were enrolled in this study in addition to 56 healthy subjects.Serum 8-OHdG and TP53 expression by ELISA were markedly elevated in HCC patients compared to LC and healthy individuals (p<0.0001). A significant correlation was noted for 8-OHdG and TP53 with disease progression and tumor differentiation but not with tumor site. 8-OHdG and TP53 were highly (p<0.05) predicting for HCC at early stages and the diagnostic performance for discriminating HCC from LC by ROC curve showed the best AUC was recorded for 8-OHdG (0.745) followed by TP53 (0.667) with accuracy (87.2% and 82% respectively). Therefore, HCV-induced oxidative DNA damage could increase the carcinogenic potential of HCC development through the activation of TP53.

8-hydroxy-2-deoxyguanosine-and-TP53-in-Egyptian-Patients-with-Hepatitis-C-Viral-Chronic-Liver-Diseases-Insight-into-the-Pathogenesis-and-Predictive-Force-converted

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Vol3 No.1 -2 : Hepatitis Delta Virus among Hepatitis B Virus Infected Patients in Jordan: Where Do We Stand?

By : ’Samer F. Swedan, 2Ziad A. Elnasser and ’Majd N. Brik Hnidi

Abstract

Infection by Hepatitis Delta Virus (HDV) which can only replicate in the presence of Hepatitis B Virus (HBV) is a cause of significant morbidity and mortality worldwide. Prevalence of HDV in Jordan is lacking. Blood samples were randomly obtained from 100 patients seropositive for active HBV. Sera were analyzed using highly sensitive and specific ELISA kits for the presence of specific HBV and HDV antigens and antibodies. Overall, samples were collected from 70 males and 30 females with an average age of 37.9 years and an age range of 13-62 years. The 94% of samples were from individuals older than 18 years. All samples were confirmed for the presence of active HBV infection. The 1 and 3% of the HBV infected individuals demonstrated markers of active and recovered HDV infection, respectively. These findings suggest that HBV positive individuals have a small but significant risk of acquiring HDV. We recommend the implementation of periodical testing for HDV infection among all HBV infected individuals and the administration of the HBV vaccine to unvaccinated individuals.


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