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Vol.8 No.3 – 7:Detection of DNA damage by SCD and Rate of Apoptosis DNA by Gel Electrophoresis among infertile males

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By: Nehad Nabil Eskarous1, Sobhy El-Sayed Hassab El-Nabi1, Mohamed Ahmed Abd El Salam2, Khaled Geba1, and Sameh Fayek GamalEl Din2

1- Genetic Engineering and Molecular Biology Department of Zoology, Faculty of Science Menoufia University, Egypt

2- Department of Andrology, Sexology & STDs, Faculty of Medicine, Cairo University, Egypt

Abstract:

Background: DNA damage as Fragmentation has adverse effects on fertilization and embryo development, so it is one of the main causes of a male factor for infertility. Several techniques have been mentioned to elevation this damage. In our study, we determine DNA damage in human spermatozoa by sperm chromatin dispersion (SCD) method and Apoptosis of DNA in human spermatozoa by Optical density in gel electrophoresis in male infertility. Objects and Methods: Semen samples were collected from 100 men and were analyzed by standard light microscopic according to the World Organization (5th edition) for diagnostic fertility. Furthermore, Sperm DNA damage was determined by using Halosperm Kit, then assessment apoptosis by optical density in Gel Electrophoresis. Results: The mean value of DNA by SCD method in infertile males increased with a value of 47.95±10.96 %   when compared with the control value of 21.2 ±2.64 % with (p< 0.00001). On the other hand, the mean value of DNA by measurement of Optical density in Gel Electrophoresis in infertile males decreased with a value of 120.27±18.73 when compare with the control value of 144.4±45 with (p =0.833). Conclusion: The assessment of sperm DNA damage by SCD method and other methods for detection of DNA apoptosis by gel electrophoresis addition to routine semen analysis play important role in the diagnosis and management of male infertility. 

Detection-of-DNA-damage-by-SCD-and-Rate-of-Apoptosis-DNA-by-Gel-Electrophoresis-among-infertile-males

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Vol.8 No.1 – 4: Protective Effects of Ambrosia maritima and Allium sativum Plant Extracts on Different Tissues of Envenomed mice with Leiurus quinquestriatus Scorpion Venom

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By: Nahed M. M. Emam*andAljohara M. Alotaibi **

*Department of Zoology, Faculty of Science, Al Arish University, Egypt.

** Department of Biology, Faculty of Science, Princess Nora Bint Abdel Rahman University, Saudi Arabia

** Department of Biology, Faculty of Science, Princess Noura Bint Abdulrahman University, Riyadh 13225, Saudi Arabia

Abstract

Background: Scorpion envenomation is a common hazard in many parts of the world and in Sinai, hence great attention must be directed towards such animals to avoid or reduce their dangers. One of the most common antioxidant medicinal plants in Sinai is the Ambrosia maritima or Damsisa plant; it is playing an important rolein reducing or neutralizing the toxic effects of venoms. Also, the Allium sativum plant or (garlic) has been used for many years to treat a variety of medical problems. Aim of work: the present study was prepared to illustrate the protective effect of damsisa and garlic plant extracts in different tissues of mice envenomed with Leiurus quinquestriatus scorpion venom. Materials and methods: 48 albino mice were divided into 6 groups. Group I: the healthy control rats received tap water for 4 weeks, and then intramuscularly (i.m.) injected with saline solution and sacrificed after 48 hours from an injection. Group II: envenomed mice were intramuscularly injected with (0.15 µg/g b.wt.) dose of the scorpion venom and sacrificed after 48 hours from envenoming.  Group III: mice treated with a daily oral dose of Damsisa plant extract (100 mg/kg) for 4 weeks; Group IV was treated with a daily oral dose of garlic plant extract (220 mg/kg) for 4 weeks. Group V: the envenomed mice were injected with the same dose of the scorpion venom and pretreated with oral doses of Damsisa (100 mg/kg b. wt) for 4 weeks. Group VI: the envenomed mice were injected with the same dose of the scorpion venom and pretreated with an oral dose of garlic plant extract (220 mg/kg b. wt) for 4 weeks. All mice were sacrificed after 48 hours from envenoming. At the end of the experiment, some tissues samples from skeletal muscles, testis, and lung tissues were collected for histopathological, immunohistochemical, and DNA ladder assay. Results: envenomed mice had severe cellular degeneration, cytoplasmic vacuolization, cellular infiltrations, and marked dilatation of blood vessels in the skeletal muscles, testis, and lung tissues. This result is confirmed with extreme immunohistochemical changes in lung tissues and the results of the DNA ladder assay revealed increased DNA fragmentation in brain tissues. The envenomed and plant-treated mice revealed marked diminished effects in histopathological alternations in the studied tissues compared to envenomed mice and reduced DNA fragmentation in brain tissues. Conclusion: This study concluded that Ambrosia maritima (D) and Allium sativum (G) plants have a protective effect against scorpion envenomation and especially the garlic plant showed the best results. Our results suggested that the ameliorative effects of these plant extracts may be due to the antioxidant and anti-inflammatory properties of these plants in combating free radical-induced oxidative stress and tissue injury resulting from envenomation.

Protective-Effects-of-Ambrosia-maritima-and-Allium-sativum-Plant-Extracts-on-Different-Tissues-of-Envenomed-mice-with-Leiurus-quinquestriatus-Scorpion-Venom-6

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