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Vol.9 No.4-13: Treatment with Leiurus quinquestratus scorpion venom ameliorates the histopathological changes of type-2 diabetic rats’ splenic tissues.

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By: Wesam Mohamed Salama*, Sabry Ali El-Naggar, Ghada Abd-Elhamid Tabl, Lamiaa Mohamed Mahmoud El Shefiey, Nabila Ibrahim El-Desouki

Department of Zoology, Faculty of Science, Tanta University, Tanta 31527, Egypt

Abstract

Type 2 diabetes mellitus (T2-DM) is a chronic metabolic disorder characterized by tissue resistance to insulin action. Leiurus quinquestriatus venom (LQV) contains a variety of bioactive components that can be used for the treatment of various diseases. This study aimed to determine the ameliorative role of LQV on the histopathological changes in splenic tissues of T2-DM rats. Forty male Sprague Dawley rats were divided into 4 groups (n = 10) as follows; Gp1 (Control group) was fed on a normal balanced diet. Gp2 (Diabetic group) was fed on a high-fat diet (HFD) for 12 weeks and then injected with streptozotocin (STZ) (30 mg/kg) i.p. Diabetic Gp3 and Gp4 groups had been injected with metformin (Met) (150 mg/kg) or LQV (1/10 of LD50), respectively. After two months of treatment, the total body weight relative splenic weight changes, and blood glucose. Also, histopathological and immunohistochemical investigations (CD-3 immunoreactive antibody) in splenic tissues were examined. The results showed that induction of T2-DM in rats led to a significant decrease in the total body weight (-38.56%), relative spleen weight (0.23 ± 0.03), and increase in the level of blood glucose (382.56 ± 2.77 mg/dL). In addition, several histopathological and immunohistochemical changes were observed in splenic diabetic tissues. The treatment of T2-DM rats with LQV led to an improvement in the total body weight of rats (4.07%), relative spleen weight (0.40 ± 0.02), decrease in the blood glucose levels (115.47 ± 1.07 mg/dL), ameliorated the histopathological and immunohistochemical changes occurred in splenic tissues of T2-DM rats.

Treatment-with-Leiurus-quinquestratus-scorpion-venom-ameliorates-the-histopathological-changes-of-type-2-diabetic-rats-splenic-tissues

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Vol.4 No.2 – 6 : Measurement the concentration of calcium in the serum of diabetic patients in Missan province, Iraq

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By : Farah Mjbali jabber

       College of Pharmacy , University of Missan , Iraq

Abstract

This study was conducted to measure the concentration of calcium  in the serum of diabetic patients, calcium is one of trace elements, by studying 50 samples representing the group of patients divided into three groups by age groups, the first group of age (4-20) years, the second group of the age of ( 21-50) years, the third group age     (51-75) Year of both sexes compared to 30 samples taken from healthy individuals as a control group. The concentration of calcium was measured for patients and healthy people.

Aim :-  The aim of this study was to measure and compare the serum levels of calcium in normal individuals and in diabetic patients.

Measurement the concentration of calcium in the serum of diabetic patients in Missan province

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Vol.2 No.10 -1 : Effect of anti-sca-1+ on diabetic rat histological, immunohistochemical and biochemical evaluation.

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By : Bahgat El-Fiky , Saber A. Sakr , Sobhy Hassab El Nabi , Mona El Gawish , Asmaa Uonis and Ahmed EL Kattan.

Abstract

Diabetes mellitus is a disease of metabolic dysregulation most notable glucose metabolism accompanied by long term complications. The present study was carried out to investigate the potential effect of Anti-Sca-1+ stem cells on type 1 diabetes induced in albino rats. The study was carried on 7 male and 24 female Albino rats (150 ± 5g). The female albino rats were divided into three main groups: normal control, diabetic group and diabetic rats treated by Anti-Sca-1+. Induction of diabetic rats was carried out by single dose injection of fasting rats for 48 h with a diluted solution of 40 mg/kg of alloxan monohydrate (2%) in freshly prepared saline solution. Diabetic rats treated group was slaughtered into two phases; the first phase after 15 days of treatment, and the second phase after 30 days of treatment. Tissue samples were separated and kept in absolute 10% formalin solution for histological and immunohistochemical studies. SRY gene expression and biochemical study of glucose tolerance, c-peptide, insulin, glycated hemoglobin and glucose transporter 2 was evaluated. The results showed that, treatment of diabetic rats, with derivative bone marrow (Anti-Sca-1+) could greatly ameliorate glucose, insulin, c-peptide, HbA1c and glut2, Hafter 15 and 30 days of treatment, as compared to normal control group and these were confirmed by histological and immunological investigations, which indicate significant progress in the pancreas tissue, by marked significant increase of pancreatic islets size and improvement in the acinar cells after 30 days of treatment by Anti-Sca-1+.

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