Posts

Vol.5 No.2 – 8 : The curative effect of Cymbopogon citrates volatile oil against chlorambucil drug toxicity

/0 Comments/in , , /by

By: ZakariaTeleb1, KamiliaTaha2, Sobhy Hassab El-Nabi3, Islam El-Garawani3, Gouda T. Dawoud2, Samraa S. El-Shafey3 and Hanaa M. El-Esawy3

1 Department of Biochemistry, National Organization for Drug Control and Research, MHP, Egypt.

2 Department of Phytochemistry, National Organization for Drug Control and Research, MHP, Egypt

3 Department of Zoology, Faculty of Science, Menoufia University, Egypt.

Abstract

Chlorambucil (CLB) is a bifunctional alkylating drug widely used as an anticancer agent and immunosuppressant. CLB mutagenicity, teratogenicity and carcinogenicity are indicated based on their structure and clinical history. This study aims to evaluate the antigenotoxic effect of Cymbopogon citratus essential oil, CC, (75 mg/kg) against CLB (7.5 mg/kg) genotoxicity in rats. GC/MS for essential oil has identified 19 compounds representing approximately 99.7% Geranial was the most abundant (53.5%) followed by Neral (35%) and Myrcene (5.3%).  The lowest was α-Muurolene (0. 1%). The marked damage was observed in total genomic DNA and total protein profile of CLB-intoxicated rat’s spleen tissues. Lymphocytes single strand breaks of treated rats were examined by comet assay after CC had ameliorated these effects in a time dependent manner (5, 10 and 15 days) for spleen and after 48 hours for lymphocytes. In conclusion, this study suggests that Cymbopogon citrates oil possesses antigenotoxic potential in CLB-intoxicated rats. It can constitute natural, new and safe co-therapeutics.

The curative effect of Cymbopogon citrates volatile oil against chlorambucil drug toxicity-converted

Download PDF

Vol.1 No.6 -7 : Ameliorative effect of Cymbopogon citratus extract on cisplatin-induced genotoxicity in human leukocytes

/0 Comments/in , , /by

By : Islam M. El-Garawani

Abstract

Cisplatin (Cis) as anticancer drug has many side effects and non-selective targeting of cancerous cells. In this work, the antigenotoxic potential of lemon grass (Cymbopogon citratus) leaves aqueous extract (CC) against the cisplatininduced genotoxicity in human peripheral leukocytes was investigated in vitro. Firstly, the antioxidant activity of plant extract was investigated by DPPH method which proved a good antioxidant potential of CC extract. CC enhanced the proliferation and increased cell count when compared to the control and Cis-treated groups. Furthermore, the expression of nuclear protein Ki-67 as a marker of cell proliferation was tested immunocytochemically and the results supported the ameliorative effect of CC against the genotoxic effect of Cis. The nuclear dual acridine orange/ethidium bromide fluorescent staining of cells showed the distinct morphological features of apoptosis and cell death among Cis-treated groups. The reduction of total genomic DNA fragmentation on agarose gel was noted among CC-treated groups when compared to Cis-treated groups.

7. Ameliorative effect of Cymbopogon citratus extract on cisplatin-induced genotoxicity in human leukocytes
Download Issue