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Vol.5 No.3 – 3 :Curcumin suppresses cellular adhesion and migration of A549 lung cancer cells via a LIMK1/MLCK dependent mechanism

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By: Ahmed A. Soffar 1, Cecil A. Matta 2, Saleh O. Albatati 3

1. Division of Molecular Biology, Department of Zoology, Faculty of Science, Alexandria University, Alexandria, Egypt.

2. Department of Zoology, Faculty of Science, Alexandria University, Alexandria, Egypt.

3. Basic Medical Sciences Department, College of Medicine, Mukalla-B.O: (50512-50511), Hadhramaut, Yemen.

Abstract

Cancer cell migration is a major cause of mortality in lung cancer patients. Despite intensive research regarding its efficacy in cancer treatment, the anti-metastatic properties of curcumin have been poorly investigated. Therefore, this work aims to explore the potential anti-migratory and anti-adhesive properties of curcumin on lung cancer cells. We also investigated the underlying molecular mode of action of curcumin. We performed scratch and adhesion assays to investigate the migratory and adhesive potentials of A549 cells. The cellular topological differences upon curcumin treatment were investigated using Scanning Electron Microscope. We also investigated the molecular mechanism triggered by curcumin using quantitative real-time PCR. In addition, we performed MTT toxicity assay to explore the toxic potential of curcumin on cancer cells. Student’s t-test was applied for evaluating the data significance using Microsoft Excel 2016. Our results showed that curcumin attenuates migration and adhesion of A549 cancer cells at non-toxic concentrations. In coincidence, the scanning electron microscope study showed a decreased density of lamellipodia and filopodia upon curcumin treatment. Interestingly, we found that the expression levels of LIMK1 and MLCK genes were downregulated upon curcumin application. Taken together, curcumin inhibits the migration and adhesion abilities of lung cancer cells and could possibly be used as a therapeutic agent against cancer cell migration. The underlying mechanism involves modulation of the expression levels of critical molecular targets including LIMK1 and MLCK proteins.

Curcumin suppresses cellular adhesion and migration of A549 lung cancer cells via a LIMK1 MLCK dependent mechanism-converted

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Vol.2 No.07 -9 : Molecular and cytochemical comparative assessment between the two food additives,sunset yellow and curcumin-induce testicular toxicity in mice.

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By : Mohamed A. Ismail

Abstract

The present work was planned to study the effects of E110 (sunset yellow) as a common synthetic in Egypt and E110 (curcumin) as a natural food-drug colorants on the testis of the male mouse. The plan of work was designed to cover six parameters: histopathological, cytochemical (involving DNA and total proteins), testis weight, sperm parameters (i.e., sperm abnormalities and sperm motility), and measuring testosterone levels in blood sera. The mice were divided into three groups, ten per each. The first group remained as controls, whilst the second orally given sunset yellow-E110 (30 mg/kg b.wt/day) as SY-group and the third one E100 ‘CU-group’ also gavage 37 mg/kg b.wt., both fed on their acceptable daily intake (ADI) dosages for 60 days. The results detected that SY revealed distinct alterations in the desired parameters, particularly histological changes in structure of seminiferous tubules such as vacuolation, necrosis and multinucleate cells. Whilst, the cytochemical DNA and proteinic profiles of the SY-treatment mice exhibited severe damage in the DNA and total protein configurations. However, such deteriorations in the spermatogenic epithelia were also approved with changes in the other criteria after administration with E110. From such alterations, the E110 recorded a highly significant increase (P< 0.0001) in the abnormalities of sperm morphology and motility. Moreover, the testosterone levels in sera of male mice indicated the significant differences among groups. The molecular protocol manifested SY (E110) - induced DNA polymorphic changes in confrontation with control by primer OPC07, whilst CU (E100) kept on the control pattern. In conclusion, the present study explored the possibility of using the applied six parameters to assessment and differentiate between the two food flavours indicating that E100 (CU) is more biosafe than the synthetic additive E110 (SY).

9_Molecular_and_cytochemical_comparative_assessment_between_the_two_food_additives_sunset_yellow_and_curcumin-induce_testicular_toxicity_in_mice
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