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Vol.5 No.2 – 8 : The curative effect of Cymbopogon citrates volatile oil against chlorambucil drug toxicity

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By: ZakariaTeleb1, KamiliaTaha2, Sobhy Hassab El-Nabi3, Islam El-Garawani3, Gouda T. Dawoud2, Samraa S. El-Shafey3 and Hanaa M. El-Esawy3

1 Department of Biochemistry, National Organization for Drug Control and Research, MHP, Egypt.

2 Department of Phytochemistry, National Organization for Drug Control and Research, MHP, Egypt

3 Department of Zoology, Faculty of Science, Menoufia University, Egypt.

Abstract

Chlorambucil (CLB) is a bifunctional alkylating drug widely used as an anticancer agent and immunosuppressant. CLB mutagenicity, teratogenicity and carcinogenicity are indicated based on their structure and clinical history. This study aims to evaluate the antigenotoxic effect of Cymbopogon citratus essential oil, CC, (75 mg/kg) against CLB (7.5 mg/kg) genotoxicity in rats. GC/MS for essential oil has identified 19 compounds representing approximately 99.7% Geranial was the most abundant (53.5%) followed by Neral (35%) and Myrcene (5.3%).  The lowest was α-Muurolene (0. 1%). The marked damage was observed in total genomic DNA and total protein profile of CLB-intoxicated rat’s spleen tissues. Lymphocytes single strand breaks of treated rats were examined by comet assay after CC had ameliorated these effects in a time dependent manner (5, 10 and 15 days) for spleen and after 48 hours for lymphocytes. In conclusion, this study suggests that Cymbopogon citrates oil possesses antigenotoxic potential in CLB-intoxicated rats. It can constitute natural, new and safe co-therapeutics.

The curative effect of Cymbopogon citrates volatile oil against chlorambucil drug toxicity-converted

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Vol.2 No.07 -8 : Ameliorative potential of β-1,3-D-Glucan on acrylamide-induced cytogenetic alterations in mice.

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By : Mohamed A. Ismail

Abstract

Acrylamide (AA) is formed in food during heat preparation (frying and baking), causing DNA toxicity. So, the aim of this study is applied β-1,3-D-Glucan (BDG) as a natural polysaccharide ameliorative to reduce the DNA hepatotoxicity and genotoxicity of bone-marrow chromosomes in male mice by using three parameters: alkaline comet assay, cytochemical DNA and cytogenetical protocols. The AA-oral fed mice are classified into three groups, the first received low dose and the second intake the double fashion of AA in alone or concomitant with BDG for 30 days, besides the fourth group of controls. The study observed that AA induced both numerical and structural chromosomal aberrations in a significant increase (p<0.05 or p<0.0001) in a dose-dependent relationship. The cytochemical study on DNA exhibited that the AA-treated hepatocyte nuclei, showed strong stainability with condensed DNA inclusions and releasing outside their nuclear envelops. Under the comet assay conditions, AA-treated hepatocytes revealed a distinct comet tail electrophoretic migration of DNA fragments that resulted from AA-induced DNA strand breaks. The study also observed similarity configurations of AA-DNA fragmented damage between the findings of cytochemical DNA and comet assays in hepatocytes, and reinforced with the stretching and pulverized chromosome aberrations. But, after enhancing with BDG, the most implications of AA were inclined into mitigation as detected by microscopical and by 3D-comet image analysis, to indicate the potential alleviation role of BDG on AA-induced DNA alterations in hepatocytes and chromosomes of mice.

8_Ameliorative_potential_of_β-1_3-D-Glucan_on_acrylamide-induced_cytogenetic_alterations_in_mice
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Vol.1 No.4 -4 : Developmental defects of cerebral cortex in maternally diabetic and aluminum intoxicated rat pups and their amelioration by Morus alba.

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By : Gamal Badawy1, Hassan El-Sayyed2, Sobhy Hassab Elnabi1, Ibrahim El-Elaimy1,Eman Al Shehari

Abstract

The present study aimed to investigate the possible ameliorative effect of crude water extract of Morus alba leaves on developmental defects of cerebral cortex of rat pups maternally subjected to diabetes and/or aluminum (Al) intoxication. One hundred and forty fertile male and virgin female albino rats were used for the experimentation. The pregnant rats were arranged into seven groups. The study included five integrated approaches and all proved that treatment with Morus alba leaves extract led to a marked amelioration for the developmental defects caused by either diabetes or Al intoxication at all levels of the study. At the ultrastructural level, the cerebral cortex of the latter exhibited massive cytological alterations of Purkinje cells (PC) and Karyolysis of chromatin material. The cytoplasm appeared translucent and enclosed by either fragmented or vesiculated RER. Mitochondria appeared electron-dense with ill-differentiated cristae. Cytoplasmic vacuoles were detected. However, pups of mothers received Morus alba extract and subjected to Al intoxication and/or diabetes showed partial amelioration of cytological structure of PC. The nuclear envelope was regularly arranged and enclosed by normal euchromatin. The cytoplasmic organelles were rearranged and approximately restored their pattern. It can therefore be concluded that leaves extract of Morus alba has marked ameliorative effects against developmental defects of cerebral cortex in pups of diabetic and Al intoxicated pregnant albino rats.

4. Developmental defects of cerebral cortex in maternally diabetic and aluminum intoxicated rat pups and their amelioration by Morus alba.
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