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Vol.8 No.4 – 6:Potential Role of Circulating Dermokine and Bcl-2 Anti-apoptotic Protein in Colorectal Cancer Egyptian Patients: Correlative Analysis with the Clinicopathological Parameters

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By: Shaimaa M. Abdelsamea1, Hoda M. El-Emshaty2, *, Othman A. Othman1, Mohamed M. El-Hemaly2, Hisham Ismail1

1Biochemistry Division, Chemistry Dept., Faculty of Science, Minia University, Minia 61519, Egypt.

2Gastrointestinal Surgery Center, Faculty of Medicine, Mansoura University, Mansoura 35516, Egypt.

Running title: Circulating Dermokine and Bcl-2 in CRC Egyptian Patients

Abstract:

Colorectal cancer (CRC) is the seventh most common cancer in Egypt, and more than half of the patients are under the age of 50. Here, we aimed to assess the levels of circulating Dermokine (DMKN) and cytoplasmic anti-apoptotic protein, Bcl-2 for detecting CRC in the earlier stages possible.The levels of DMKN, Bcl-2, Carcinoembryonic antigen (CEA), and Carbohydrate antigen 19.9 were determined using ELISA in the sera of 53 CRC patients, 18 ulcerative colitis patients, and 24 healthy individuals. Statistical analyses were performed using the SPSS program. Serum levels of DMKN and Bcl-2 were significantly higher (p < 0.0001) in CRC patients than in non-cancer individuals. Highly significant correlations were recorded between levels of DMKN and Bcl-2 and the pathological TNM tumor characteristics. At the best cut-off level (68-pg/mL), the DMKN assay showed high degrees of sensitivity (87%), specificity (100%), and accuracy (91%) in comparison with investigated biomarkers. Furthermore, regression analysis revealed a DKB-Score based on DMKN and Bcl-2 with an AUROC of 0.991. The developed score showed a high degree of efficiency (97.4%) for discriminating CRC patients from controls. In conclusion, the assessment of serum DMKN either alone or simultaneously with Bcl-2 has a potential role in discriminating CRC from premalignant patients.

Potential-Role-of-Circulating-Dermokine-and-Bcl-2-Anti-apoptotic-Protein-in-Colorectal-Cancer-Egyptian-Patients-Correlative-Analysis-with-the-Clinicopathological-Parameters-2

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Vol.6 No.2 – 5: Role of Human Pituitary Tumor-Transforming Gene1 (HPTTG1) as a prognostic biomarker for metastasis in Egyptian patients breast, colon and Liver cancer

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By: Samir Ali Mohamed El-Masry1, Sherif Mohsen Elsherbini1,

Abdelmoneam Ahmed Abdelmoneam2, Ahmed Gouda Ibrahim Radwan1

1-Molecular Biology department, Genetic Engineering, and Biotechnology Research Institute, Sadat City University, Egypt, 32897

2- Haemato-Oncology department, Faculty of Medicine, Banha University, Egypt, 13511

Abstract

hPTTG1 is an oncogene that overexpressed in most human carcinomas. It was reported to be involved in cell cycle regulation and sister chromatid separation.  PTTG expression level has been associated with tumor progression, invasion, and metastasis. In this study, hPTTG1 expression was analyzed in three cancer patients’ groups including; breast cancer, colorectal cancer, and hepatocellular carcinoma patients using RT-PCR. Our data revealed that the expression of PTTG1 was high in three groups; however, the metastatic cases reported higher expression levels of PTTG1 compared to non-metastatic groups. Thus, PTTG1 could be a prognostic marker in different types of cancer patients, and targeting PTTG1 might be a good strategy against metastasis.

Role of Human Pituitary Tumor-Transforming Gene1 (hPTTG1) as a prognostic biomarker in Egyptian patients breast, colon and liver cancer.

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Vol.5 No.2 – 3 : Efficiency of collagen III, metalloproteinase 1, carcinoembryonic antigen and carbohydrate antigen 19.9 for colon cancer diagnosis

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By; Abdelfattah M. Attallah1, Mohamed A. El-Far2, Mohamed M. Omran3, Mohamed A. Abdelrazek 1, Kareem  A. Attallah1, Mohamed S. Elbendary1, Sara A. Soliman1, Rehab A. Atwa1

1Research & Development Department, Biotechnology Research Center, New Damietta City, Egypt

2Department of Chemistry, Faculty of Science, Mansoura University, Mansoura, Egypt

3Department of Chemistry, Faculty of Science, Helwan University, Cairo, Egypt

Abstract

Background: Currently, blood markers are noninvasive methods for diagnosis of patients with colorectal cancer (CRC). We evaluated four biomarkers (Carcinoembryonic antigen (CEA), carbohydrate antigen 19.9 (CA19.9), collagen III and metalloproteinase 1 (MMP-1)) and their combination as substitute method to enhance diagnosis of CRC. Subjects and methods: one hundred ninety five patients had undergone colonoscopy examination were inclusive in the study (135 CRC and 60 benign growths). In addition, 45 healthy individuals were included.  Multivariate discriminant analysis (MDA) and area under the receiver operating characteristic curve (AUC) were applied for evaluation the diagnostic power of single and their combination. Results:  levels of collagen III, CEA and CA 19.9 increased while MMP-1 decreased with  progression of CRC  (stages, positive lymph node invasion, distant organ metastasis and high grades)  with a significant difference (P <0.01- P < 0.0001). Combination of MMP-1, CEA, CA 19-9 and Collagen III yielded MC3 index had diagnostic power greater than each single marker could achieve alone. When differentiate colon cancer from benign growth, AUC of MC3 index was 0.91 yielded 85.2% sensitivity and 86.7% specificity. Moreover, MC3 index for patients with late stages, lymph node invasion, organ metastasis and high-grade had AUC = 0.81, 0.82, 0.80 and 0.80 higher than CEA and CA19.9.Conclusion:  MC3 index is a can be used as an effective index for early detection of CRC.

Efficiency of collagen III, metalloproteinase 1, carcinoembryonic antigen and carbohydrate antigen 19.9 for colon cancer diagnosis

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