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Vol.8 No.2 – 7:The Role of CD3 and CD8 in Preterm Preeclamptic women by using Immunohistochemical technique

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By: Alaa Saadi Abbood

Department of Biology, Al-Farabi University College (FUC), Al-Dora Square, Al-Masafee Street, Baghdad, Iraq

Abstract

Preterm birth (PTB) and PE are the two major causes of perinatal mortality, morbidity, and long-range neurological disability. PTB defined as delivery before 37 weeks gestation, has become an epidemic in developed countries, Indeed PTB and PE are leading causes of maternal and neonatal death worldwide. The immunohistochemical study show: – The staining intensity for PE immunohistochemical showed the greatest (+2) intensity was recorded at 52% for anti-CD3 lymphocyte biomarker. Also, followed by 28% of (+1) Intensity was comparable biomarkers. (12%) of +3 and 8% for 0 intensity when compared with Tonsil control positive and placental tissue control negative. On the other hand, the staining intensity for PE immunohistochemical shows the greatest frequency of (0) intensity was recorded for anti-CD8 cytotoxic T-cell biomarker with 52 % then +1 (28%) and 20% for +2 when compared with skin control positive and negative.

The Role of CD3 and CD8 in Preterm Preeclamptic women by using Immunohistochemical technique (1)

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Vol.7 No.3 – 3: T-lymphocyte subsets (CD3+, CD4+, and CD8+) in Systemic Lupus Erythematosus (SLE): Correlation with Clinical Manifestation

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By: Abeer M. El-Maghraby1, Yasser B.M. Ali2, Eman A. El-maadawy2, Mohamed F. Elshal2, Iman H Bassyouni3, Islam M El-Garawani1, Roba M. Talaat2

1Zoology Department, Faculty of Science, Menoufia University, Shebin El-Kom 32511, Menoufia, Egypt

2Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Egypt

3Rheumatology and Rehabilitation Department, Faculty of Medicine, Cairo University, Cairo, Egypt

Abstract

AIM: Systemic lupus erythematosus (SLE) is a systemic autoimmune disease that has a multifactorial etiology. T- Lymphocytes are essential in SLE pathogenesis. It plays a crucial role in autoantibody production and the subsequent immune complex formation, which may induce or directly damage multiple organs. This study was carried out aiming to quantify certain T lymphocyte subsets (CD3+, CD4+, and CD8+) in SLE patients and to elucidate if there is a possible influence of disease activity scores and clinical manifestations. Patients and Methods: This study included 100 SLE patients with various disease activity scores (SLEDAI) and 100 healthy age and sex-matched controls. The frequency of CD3+, CD4+, and CD8+ was assessed by flow cytometry. Results: A significant up-regulation in CD3+ (P<0.01), CD8+ (P<0.001) coincides with a significant downregulation in CD4+cells (P<0.001) were detected in SLE patients compared to controls. A significant up-regulation in CD4+ (P<0.05) was demonstrated in active SLE patients compared with the inactive form of the disease. On the other hand, no significant change was observed in the frequency of CD3+ and CD8+T cell subsets between active and inactive patients. Arthritic patients have a significant reduction in CD3+ and CD4+ T cells while those with Vasculities significantly reduce in CD4+, CD8+ compared with SLE patients without these manifestations.   Conclusion: The current study results stressed the importance of T cell subsets in SLE disease. They might participate in disease activity and in controlling several manifestations of lupus. Our findings will help design more studies on the role of T cell subsets in SLE pathogenesis which may provide new therapeutic targets for SLE.

T-lymphocyte-subsets-CD3-CD4-and-CD8-in-Systemic-Lupus-Erythematosus-SLE-Correlation-with-Clinical-Manifestation-converted

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