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Vol.9 No.4-13: Treatment with Leiurus quinquestratus scorpion venom ameliorates the histopathological changes of type-2 diabetic rats’ splenic tissues.

By: Wesam Mohamed Salama*, Sabry Ali El-Naggar, Ghada Abd-Elhamid Tabl, Lamiaa Mohamed Mahmoud El Shefiey, Nabila Ibrahim El-Desouki

Department of Zoology, Faculty of Science, Tanta University, Tanta 31527, Egypt

Abstract

Type 2 diabetes mellitus (T2-DM) is a chronic metabolic disorder characterized by tissue resistance to insulin action. Leiurus quinquestriatus venom (LQV) contains a variety of bioactive components that can be used for the treatment of various diseases. This study aimed to determine the ameliorative role of LQV on the histopathological changes in splenic tissues of T2-DM rats. Forty male Sprague Dawley rats were divided into 4 groups (n = 10) as follows; Gp1 (Control group) was fed on a normal balanced diet. Gp2 (Diabetic group) was fed on a high-fat diet (HFD) for 12 weeks and then injected with streptozotocin (STZ) (30 mg/kg) i.p. Diabetic Gp3 and Gp4 groups had been injected with metformin (Met) (150 mg/kg) or LQV (1/10 of LD50), respectively. After two months of treatment, the total body weight relative splenic weight changes, and blood glucose. Also, histopathological and immunohistochemical investigations (CD-3 immunoreactive antibody) in splenic tissues were examined. The results showed that induction of T2-DM in rats led to a significant decrease in the total body weight (-38.56%), relative spleen weight (0.23 ± 0.03), and increase in the level of blood glucose (382.56 ± 2.77 mg/dL). In addition, several histopathological and immunohistochemical changes were observed in splenic diabetic tissues. The treatment of T2-DM rats with LQV led to an improvement in the total body weight of rats (4.07%), relative spleen weight (0.40 ± 0.02), decrease in the blood glucose levels (115.47 ± 1.07 mg/dL), ameliorated the histopathological and immunohistochemical changes occurred in splenic tissues of T2-DM rats.

Treatment-with-Leiurus-quinquestratus-scorpion-venom-ameliorates-the-histopathological-changes-of-type-2-diabetic-rats-splenic-tissues

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Vol.8 No.2 – 7:The Role of CD3 and CD8 in Preterm Preeclamptic women by using Immunohistochemical technique

By: Alaa Saadi Abbood

Department of Biology, Al-Farabi University College (FUC), Al-Dora Square, Al-Masafee Street, Baghdad, Iraq

Abstract

Preterm birth (PTB) and PE are the two major causes of perinatal mortality, morbidity, and long-range neurological disability. PTB defined as delivery before 37 weeks gestation, has become an epidemic in developed countries, Indeed PTB and PE are leading causes of maternal and neonatal death worldwide. The immunohistochemical study show: – The staining intensity for PE immunohistochemical showed the greatest (+2) intensity was recorded at 52% for anti-CD3 lymphocyte biomarker. Also, followed by 28% of (+1) Intensity was comparable biomarkers. (12%) of +3 and 8% for 0 intensity when compared with Tonsil control positive and placental tissue control negative. On the other hand, the staining intensity for PE immunohistochemical shows the greatest frequency of (0) intensity was recorded for anti-CD8 cytotoxic T-cell biomarker with 52 % then +1 (28%) and 20% for +2 when compared with skin control positive and negative.

The Role of CD3 and CD8 in Preterm Preeclamptic women by using Immunohistochemical technique (1)

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