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Vol.7 No.4 – 2:Diagnostic values of a model based on B-type natriuretic peptide, C reactive protein, and neutrophil-lymphocyte ratio for diagnosis of diabetic heart diseases patients

By: Mohamed M. Omran1*, Yassmin Taha2, Mohamed Kadry3, Fathy M. Eltaweel2, Tarek M. Emran4

1 Chemistry Department, Faculty of Science, Helwan University, Cairo, Egypt.

2 Chemistry Department, Faculty of Science, Damietta University, Egypt

3Laboratory Department, Gamasa Central Hospital, Gamasa, Egypt

4Clinical Pathology Department, Faculty of Medicine, Al-Azhar University,

New Damietta; Egypt

Abstract

Background: People with type 2 diabetes means they are more likely to progress many complications such as hyperglycemia, low-grade inflammation, insulin resistance, and accelerated heart disease. Diabetic heart disease (DHD) can be diabetic cardiomyopathy, heart failure (HF), and coronary heart disease.

Methods: Blood samples from 100 patients with DHD, and 76 controls included   [diabetic patients without cardiac diseases 56, and 20 healthy individuals were collected. C reactive protein (CRP) and neutrophil-lymphocyte ratio (NLR), vascular cell adhesion molecule (VCAM), B-type natriuretic peptide (BNP), were estimated in all study individuals. The area under receiver-operating characteristic curve (AUC) was used to evaluate the diagnostic accuracy of the single and combined markers. Results: Levels of CRP, BNP, and NLR had significant differences but VCAM had no significant differences among DHD, controls, diabetic, and healthy individuals groups.  Then VCAM marker was excluded from further analysis. CRP was the most efficient biomarker among markers for discriminating DHD patients from healthy individuals, diabetic patients and, controls with AUCs were 0.99, 0.87, and 0.89; respectively. We developed a new model based on three blood markers (CRP, BNP, NLR) for differentiated DHD.  Linear significant correlations were observed between model levels and candidate markers that included: CRP (r=0. 78; p <0.0001), NLR (r = 0. 0.53; p <0.0001), BNP (r = – 0.55; p <0.0001). The AUC of the model was 1.0 with 100 % sensitivity and 100 % specificity for discriminated patients with DHD from healthy individuals. For discriminate patients with DHD from diabetic patients, AUC was 0.90 with 92 % sensitivity and 81% specificity. For discriminate patients with DHD from controls, AUC was 0.93 with 92 % sensitivity and 83 % specificity.  

Conclusion: The combination of three candidate biomarkers CRP, BNP, and NLR can be used to improve the diagnosis of DHD patients with high diagnostic performances.

Diagnostic-values-of-a-model-based-on-B-type-natriuretic-peptide-C-reactive-protein-and-neutrophil-lymphocyte-ratio-for-diagnosis-of-diabetic-heart-diseases-patients-converted

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Vol.5 No.1 – 2 : Role of Monocyte Chemoattractant Protein-1 for Diagnosing Acute Myocardial Infarction

By : Mohamed M. Omrana*, Faten M. Zahranb, Mohamed Kadryc,

Arafa A. M. Belal c, Reihan M.Sd

a Chemistry Department, Faculty of Science,  Helwan University, Cairo, Egypt;

b Chemistry Department, Faculty of Science, Zagazig University, Zagazig, Egypt;

c Chemistry Department, Faculty of Science, Port Said University, Port Said, Egypt;

 d Cardiology Department, Faculty of  Medicine, New Damietta, Al-Azhar University, Egypt;

*Corresponding author: Mohamed Mostafa Omran, PhD: E-mail: drmmomran@yahoo.com

Abstract

Background:Acute myocardial infarction (AMI) controlled and promoted by inflammation within coronary plaque. Monocyte chemoattractant protein-1 (MCP-1) is pro-inflammatory mediator, that’s playing a major role in plaque rupture. This study aimed to assess the diagnostic performance of MCP-1 for early diagnosis of AMI among chest pain (CP) patients. Methods: MCP-1 and cardiac Troponin I (cTnI) were performed for all studied patients. Receiver operating characteristic (ROC) curve was used to assess the diagnostic accuracy of biomarkers. Results: Baseline level of MCP-1 has good capacity for discriminating patients with AMI from non coronary chest pain (NCCP), stable angina (SA) and unstable angina (UA)  patients with efficiency 91%, 91% and 83%; Respectively. Area under the curves (AUCs) of MCP-1 for diagnosis AMI patients at 0-6 hours and > 6-12 hours after onset time of CP were 0.69 (P < 0.001); and 0.71(P < 0.0001); respectively, compared with cTnI were 0.58 (P < 0.001) and 0.67 (P < 0.001); respectively. However, at > 12-24 hours, cTnI has AUC 0.93 (P < 0.0001) compared with MCP-1 0.74 (P < 0.0001). In conclusion: Independent early baseline MCP-1 has given sufficient diagnostic information for patients with AMI.


Role of Monocyte Chemoattractant Protein-1 for Diagnosing

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Vol.1 No.6 -10 : Ultrastructure biomarker in Anisops sardeus (Heteroptera Notonectidae) for the assessment and monitoring of Water Quality of Al-Mahmoudia Canal,Western Part of Nile Delta, Egypt

By : Dalia A. Kheirallah

Abstract

Ultrastructure biomarker reflects the effects of pollutants. The present study amid to evaluate the reproductive changes of the aquatic hemipteran insect Anisops sardeus (as a bioindicator organism), inhabiting three sites in Al-Mahmoudia canal (Abou-Hommous, Zarcon town, Manshia) which varied in physical and chemical properties. Mahmoudia canal is considered the main water source for Alexandria, which receive water from Rosetta branch at Mahmoudia city. The canal receives domestic and agriculture wastes from Zarcon Drain and other non-point sources. The present work is concerned with monitoring bioaccumulation of metal in the testes of A. sardeus using SEM-X-ray microanalysis and illustrating spermatogenesis disruptions. Insects caught from polluted sites (Zarcon town, Manshia) showed higher proportion of heavy metals in particular Cu, Zn and Hg than in the less polluted site (Abou-Hommous). Many alterations of the general architecture of the testis were pronounced. Disruption and damage for the normal cellular organization were observed. In epithelial cells, aggregated clumps of heterochromatin, irregular nuclear envelope, cytoplasm with disorganized mitochondria and convolution of follicular wall were noticed. In spermatogonia the nucleus appeared with disintegrated nucleolus, vacuolated cytoplasm and degenerative changes in the mitochondria. According to the obtained results the water quality of Al-Mahmoudia canal was lower at the polluted sites and the watercourse from south to north direction has been increased in pollution sources. The results also showed that the intensity of the histopathological changes increased with increasing the intensity of heavy metals. As a biomarker of exposure to toxicants, histopathology represents a useful tool to assess the degree of pollution


10. Ultrastructure biomarker in Anisops sardeus (Heteroptera Notonectidae) for the assessment and monitoring of Water Quality of Al-Mahmoudia Canal,Western Part of Nile Delta, Egypt
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