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Vol.8 No.3 – 8:Hematological and biochemical changes associated with water-pipe (Shisha)smoking for some volunteers in Missan province

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By: Anas, S. Abuali

Biology Department / Basic Education College / Missan University, Iraq

Abstract

    Aim of the study: Clinical and experimental studies detected that waterpipe smoking is more harmful than a cigarette with can induce oxidative stress and inflammation. The current study was performed to investigate the effect of water pipe smoking on hematological parameters and evaluation the biochemical parameters including a lipid profile, live function enzymes, alkaline phosphatase, total protein, creatinine, blood urea nitrogen, and blood glucose.

Method: The study was performed on (150) volunteers who agreed to participate in this study divided into water pipe smokers and nonsmokers aged between (20-60) years. Five (5ml) of venous blood samples were collected, each blood sample was separated into two tubes, the first tube with EDTA for hematological assessment and the second was centrifugation and the serum was stored in a -20°C freezer till handled for biochemical analysis for determining lipid profile, liver function enzymes, alkaline phosphatase, creatinine, blood urea nitrogen and blood glucose. Complete blood picture for collecting blood samples was performed by automatic methods (System X kx-21n automated hematology analyzer; JAPAN CARE CO., LTD) including hemoglobin (Hb), white blood cells (WBCs), red blood cells (RBCs), Platelets and Haematocrit or packed cell volume (PCV). Biochemical tests and lipid profile analysis were performed in laboratories of Al-Sadder Teaching Hospital in Amarha City according to the standard methods described in the Analysis Kits used in this study were products of Spanish Company Spinreact.

The results: The results observed that the water pipe smokers were in ages between 31-40 years with a percentage of 43%, followed by the aged 20-30 years with a percentage of 25%. Hematological analysis for the blood samples collected from water pipe smokers and non-smoking (control) observed a significant increase in RBCs, WBCs, HCT, Hb, and Plt in water pipe smokers as compared with the non-smoker group in (p<0.05). Lipid profile values observed a significant increase in the total cholesterol levels, (LDL), vLDL) and Triglyceride with a significant decrease in HDL (P>0.05) in water pipe smokers as compared with the non-smoker group. Significant increase in the levels of AST, ALT, and alkaline phosphatase enzyme, also the creatinine, blood urea nitrogen, and blood glucose observed a significant increase in(P>0.05) as compared with non-smokers. On other hand, there was a significant decrease in total proteins in water pipe smokers.

Conclusions: Water pipe smoking caused abnormal changes in complete blood picture (CBC) and serum lipids such as the total cholesterol and Triglyceride levels. Also harmed the liver functions and kidney functions.

Hematological-and-biochemical-changes-associated-with-water-pipe-Shishasmoking-for-some-volunteers-in-Missan-province

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Vol.6 No.4 – 2: Protective effect of omega-3 on Doxorubicin-induced hepatotoxicity in male albino rats

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By: 1Farozia I. Moussa, 1Horeya S. Abd El-Gawad, 1Salwa S. Mahmoud, 2Faiza A. Mahboub, and 1Saliha G.Abdelseyd

1Department of Zoology, Faculty of Science, Alexandria University, Egypt

2Department of Biology, Faculty of Applied Sciences, Umm Al-Qura University, Saudi Arabia

Abstract

Doxorubicin (DOX) is an antineoplastic anthracycline used to treat various forms of cancer. Although DOX is an effective chemotherapeutic agent, it has been documented to cause oxidative damage in several body organs. The present study aimed to investigate the protective effects of omega-3 against doxorubicin-induced hepatic toxicity in adult male rats. Animals were divided into four groups. The first group was orally administered with 0.5ml corn oil and served as a control group. The second group was treated with omega-3 fatty acid (400mg/kg b.w) daily for 30 days. The third group was injected intraperitoneally with a single dose of DOX (30mg/kg b.w). Animals in the fourth group were treated with omega-3 at the same dose level as those of group 2 followed by intraperitoneal injection of a single dose of DOX as in the third group. Injecting animals with DOX induces various histological changes in the liver. These changes include congestion and dilatation of blood vessels, leucocytic infiltration, cytoplasmic vacuolization, degenerated hepatocytes, and pyknotic nuclei. Moreover, DOX caused a significant elevation in serum ALT, AST, LDH, lipid profile, total bilirubin, total protein, albumin, and globulin after 4 weeks of treatment. It also caused an increase in malondialdehyde (MDA) and depletion of the antioxidant enzymes, catalase (CAT), superoxide dismutase (SOD), and reduced glutathione reduced (GSH). Treating animals with omega 3 fatty acids in combination with DOX led to an improvement in the histological and biochemical changes induced by DOX together with a significant decrease in the level of MDA and an increase in the activity of antioxidant enzymes. The results of the present work indicated that omega-3 fatty acid had a protective effect against liver damage induced by Doxorubicin and this is due to its antioxidant activities.

Protective-effect-of-omega-3-on-Doxorubicin-induced-hepatotoxicity-converted

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Vol.6 No.2 – 2: Comparative effect of extracts of Blighia sapida (Sapindaceae) from three regions on the biochemical parameters of hypertensive rats

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Yao Honoré N’GUESSAN*1,2, Wawa Justine TIEKPA3, Mandé Ryta Adélaïde DOUE2, Cassime TIEMOKO2, Chérif Moustapha KARAMOKO2, Alain Dit Phillippe BIDIE1,2, Daouda KONE1.

1 African Center of Excellence on Climate Change, Biodiversity and Sustainable Agriculture, Félix Houphouet-Boigny University, Abidjan, Ivory Coast, 22 BP 582 Abidjan 22, Ivory Coast.

2 Pharmacodynamics-Biochemical Laboratory, UFR Biosciences, Félix Houphouet-Boigny University, Abidjan, Ivory Coast, 22 BP 582 Abidjan 22, Ivory Coast.

3 Péliforo Gon Coulibaly University, BP 1328 Korhogo, Ivory Coast

Abstract

Blighia sapida is a plant with many therapeutic virtues. In Ivory Coast, this plant is widespread in several regions with different climatic conditions. The aim of our study is to compare the effects of aqueous extracts of Blighia sapida harvested in three Ivorian regions on the biochemical parameters of rats made hypertensive by a fructose diet. Rats (20) were fed a high fructose diet (70% fructose, 10% fat, 20% protein) for 30 days. Then blood pressure was measured from an armband to the tail of the rats. Finally, biochemical and lipid parameters were measured before and after the fructose-enriched diet. All rats fed the high-fructose diet had similar rates of hypertension (SBP = 160.0 ± 0.07 mmHg; DBP = 141.5 ± 1.21 mmHg; HR = 325.7 ± 1.52 beats/min) and a change in dosing parameters. Aqueous extracts of Blighia sapida from Adzopé (EAA) and Korhogo (EAK) administered to hypertensive rats normalized the previously increased cardiovascular, biochemical and lipid parameters compared to control rats (healthy and untreated diseased rats). However, the aqueous extract of Blighia sapida of Adzopé (EAA) showed the best effects on hypertension compared to the aqueous extract of Blighia sapida of Korhogo (EAK). Indeed, the more humid the region and the climate, the more effective the extract is. The regulating effect of aqueous extracts of Blighia sapida on blood pressure, therefore, differs from one region to another with different climatic conditions. This would explain its use in traditional medicine in certain climatic zones compared to others where its therapeutic effects would be unknown because less. Adzopé would, therefore, be the best region where Blighia sapida should be harvested for the optimal treatment of high blood pressure.

Comparative effect of extracts of Blighia sapida (Sapindaceae) from three regions on the biochemical parameters of hypertensive rats-converted

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